[Electroacupuncture promotes angiogenesis in MIRI rats by modulating the AMPK/KLF2 signaling pathway].

Objectives: To observe the effect of electroacupuncture(EA) on adenosine 5'-monophosphate-activated protein kinase (AMPK)/Kruppel-like factor 2 (KLF2) signaling pathway in ischemic myocardial tissues of rats, so as to explore the underlying mechanism of EA in attenuating myocardial ischemia-reperfusion injury (MIRI) through mediating angiogenesis.

Methods: Male SD rats were randomly divided into sham operation group, model group and EA group, with 10 rats in each group. The MIRI model was established by ligation of the anterior descending branch of the left coronary artery.

Risk assessment of honeybee larvae exposure to pyrethroid insecticides in beebread and honey.

Honeybee is an essential pollinator to crops, evaluation to the risk assessment of honeybee larvae exposure to pesticides residue in the bee bread and honey is an important strategy to protect the bee colony due to the mixture of these two matrices is main food for 3-day-old honeybee larvae. In this study, a continuous survey to the residue of five pyrethroid insecticides in bee bread and honey between 2018 and 2020 from 17 major cultivation provinces which can be determined as Northeast, Northwest, Eastern, Central, Southwest, and Southern of China, there was at least one type II pyrethroid insecticide was detected in 54.7 % of the bee bread samples and 43.

Electroacupuncture preconditioning protects against myocardial ischemia-reperfusion injury by modulating dynamic inflammatory response.

Background: The protective effects of electroacupuncture (EA) preconditioning against myocardial ischemia-reperfusion injury (MIRI) have been reported. However, the underlying mechanism remains unclear. Recent research has indicated that the dynamic inflammatory response following MIRI plays an essential role in the progression of myocardial injury.

Toxic effects of detected pyrethroid pesticides on honeybee (Apis mellifera ligustica Spin and Apis cerana cerana Fabricius).

To obtain the presence of environmental contaminants in honeybee and compare the toxicity of the detected pesticides to Apis mellifera ligustica Spin and Apis cerana cerana Fabricius. In this work, 214 honeybee samples were collected to simultaneous monitoring 66 pesticides between 2016 and 2017 in China. A modified QuEChERS extraction method coupled with multi-residue analytical methods by Ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and Gas chromatography-mass spectrum (GC-MS).

The effects of beta-cypermethrin, chlorbenzuron, chlorothalonil, and pendimethalin on Apis mellifera ligustica and Apis cerana cerana larvae reared in vitro.

Background: Declines in bee populations and diversity have drawn international attention. The long-term use of chemical pesticides has affected bee behavior and physiology. This study aimed to investigate the effects of chronic exposure to four commonly used chemical pesticides (beta-cypermethrin, chlorbenzuron, chlorothalonil and pendimethalin) on the growth of Apis mellifera ligustica and Apis cerana cerana larvae reared in vitro.

Analysis of honey bee exposure to multiple pesticide residues in the hive environment.

Since the loss of honeybees in hives could have a greater impact on colony health than those of their foraging bees, it is imperative to know beehives' pesticide exposure via oral ingestion of contaminated in-hive matrices. Here, a 4-year monitoring survey of 64 pesticide residues in pollen, nectar and related beehive matrices (beebread and honey) from China's main honey producing areas was carried out using a modified version of the QuEChERS multi-residue method. The results showed that 93.

MiR-9 functions as a tumor suppressor in acute myeloid leukemia by targeting CX chemokine receptor 4.

MicroRNAs (miRNAs) play key roles in the pathogenesis of many cancers, including acute myeloid leukemia (AML). Although miRNA-9 (miR-9) is involved in the leukemogenesis of AML, the underlying mechanisms remain to be elucidated. In this study, we found that miR-9 and C-X-C chemokine receptor 4 (CXCR4) were differentially expressed in myeloid leukemia, particularly in AML.

Ethyl pyruvate suppresses the growth, invasion and migration and induces the apoptosis of non‑small cell lung cancer cells via the HMGB1/RAGE axis and the NF‑κB/STAT3 pathway.

As an inhibitor of high mobility group protein B1 (HMGB1), ethyl pyruvate (EP) has been associated with various inflammatory diseases. Recent studies have investigated the relationship between EP and cancer. The present study aimed to determine the antitumor efficacy of EP in non‑small cell lung cancer (NSCLC) cells and elucidate the underlying mechanism.

Preparation and Properties of 3D Printed Alginate⁻Chitosan Polyion Complex Hydrogels for Tissue Engineering.

Three-dimensional (3D) printing holds great potential for preparing sophisticated scaffolds for tissue engineering. As a result of the shear thinning properties of an alginate solution, it is often used as 3D printing ink. However, it is difficult to prepare scaffolds with complexity structure and high fidelity, because the alginate solution has a low viscosity and alginate hydrogels prepared with Ca crosslinking are mechanically weak.

The Effect of Neonicotinoid Insecticide and Fungicide on Sugar Responsiveness and Orientation Behavior of Honey Bee () in Semi-Field Conditions.

Neonicotinoid insecticides are in widespread use around the world, cause pollinator decline. We used semi-field conditions to determine the effect of sublethal insecticide, thiamethoxam, exposure on orientation behavior and sugar responsiveness. Bees could not reject the non-treated flower or the insecticide or insecticide/fungicide treated flower.

Evaluation of Highly Detectable Pesticides Sprayed in L.: Degradation Behavior and Risk Assessment for Honeybees.

Honeybees are major pollinators of agricultural crops and many other plants in natural ecosystems alike. In recent years, managed honeybee colonies have decreased rapidly. The application of pesticides is hypothesized to be an important route leading to colony loss.

Characterization of PGRP-S1 from the oriental armyworm, Mythimna separata.

Peptidoglycan is the key component forming the backbone of bacterial cell wall. It can be recognized by a group of pattern recognition receptors, known as peptidoglycan recognition proteins (PGRPs) in insects and higher animals. PGRPs may serve as immune receptors or N-acetylmuramoyl-L-alanine amidases (EC 3.

A survey of multiple pesticide residues in pollen and beebread collected in China.

Honeybees, as major pollinators, make vital contributions to humans and ecosystems. Worryingly, a phenomenon known as honeybee colony losses has been reported in recent years. One of the factors underlying the occurrence of honeybee colony losses is exposure of honey bees to pesticide residues in their food, which cause detrimental sublethal effects and may lead to the collapse of their colonies.

Tannic acid-derived metal-phenolic networks facilitate PCL nanofiber mesh vascularization by promoting the adhesion and spreading of endothelial cells.

Synthetic polymer nanofiber meshes produced by electrospinning with a high surface area and suitable porosity mimic the basic structure of the extracellular matrix (ECM) for endothelial cell culture. However, some polymers lacking a cell adhesion surface cause defective vessel formation by cells. Herein, we evaluated metal-phenolic networks (MPNs) formed by tannic acid (TA) and metal ions (Fe ions) as nanoscale coating systems on polycaprolactone (PCL) nanofiber surfaces for the modulation of human umbilical vein endothelial cell (HUVEC) morphology and function.

Multi-Residue Analysis of Pesticide Residues in Crude Pollens by UPLC-MS/MS.

A multi-residue method for the determination of 54 pesticide residues in pollens has been developed and validated. The proposed method was applied to the analysis of 48 crude pollen samples collected from eight provinces of China. The recovery of analytes ranged from 60% to 136% with relative standard deviations (RSDs) below 30%.

Influence of early diagenesis on the vertical distribution of metal forms in sediments of Bohai Bay, China.

The influence of early diagenesis on the vertical distribution of metal forms in the sediments of Bohai Bay was discussed in this paper. The results showed that the concentrations were: Al > Fe ≈ Ca > Mn > Cr > Zn > Cu > Pb > Cd. In vertical distribution, the forms of Cr and Pb were stable from the top to the bottom.

Rabbit anti-rabies immunoglobulins production and evaluation.

Due to the disadvantages of human and equine rabies immunoglobulin, it is necessary to develop a substitute for HRIG and ERIG, especially for those people living in the developing countries. Because of higher affinity and lower immunogenicity of rabbit's immunoglobulins, anti-rabies immunoglobulins specific to rabies virus were produced in rabbits as a bioreactor, and had been characterized by ELISA, affinity assay, immunofluorescence assay (IFA), immunocytochemistry, rapid fluorescent focus inhibition test (RFFIT). ELISA, affinity assay and IFA showed that rabbit RIG (RRIG) bound specifically to rabies virions.