Improving Flow Bead Assay: Combination of Near-Infrared Optical Tweezers Stabilizing and Upconversion Luminescence Encoding.

To enhance signal acquisition stability and diminish background interference for conventional flow bead-based fluorescence detection methods, we demonstrate here an exceptional microfluidic chip assisted platform by integrating near-infrared optical tweezers with upconversion luminescence encoding. For the former, a single 980 nm laser is employed to perform optical trapping and concurrently excite upconversion luminescence, avoiding the fluctuation of the signals and the complexity of the apparatus. By virtue of the favorable optical properties of upconversion nanoparticles (UCNPs), the latter is carried out by employing two-color UCNPs (Er-UCNPs and Tm-UCNPs) with negligible spectral overlaps.

Real-Time Monitoring of Temperature Variations around a Gold Nanobipyramid Targeted Cancer Cell under Photothermal Heating by Actively Manipulating an Optically Trapped Luminescent Upconversion Microparticle.

We demonstrate an effective approach to realize active and real-time temperature monitoring around the gold nanobipyramids (AuNBPs)-labeled cancer cell under 808 nm laser irradiation by combining optical tweezers and temperature-sensitive upconversion microparticles (UCMPs). On the one hand, the aptamer-modified AuNBPs that absorb laser at 808 nm not only act as an excellent photothermal reagent but also accurately and specifically bind the target cancer cells. On the other hand, the single optically trapped NaYF:Yb, Er UCMPs with a 980 nm laser exhibit temperature-dependent luminescence properties, where the intensity ratio of emission 525 and 547 nm varies with the ambient temperature.

[The Study on Combined Screening Method for TBS and Cervical Cancer Cell’s DNA Quantitative Analysis Based on Multi-Spectral Imaging Technology].

The traditional screening method of cervical cancer mainly involves TBS classification method and quantitative analysis method based on DNA, TBS screening method which has high diagnostic rate, but it needs experienced doctors to participate in the process with low sensitivity. Therefore, it is difficult to achieve early cervical cancer screening; cell’s DNA quantitative analysis, only stained the nucleus, achieving a quantitative and automated analysis. Even with high sensitivity, the specificity is poor.

[Study of Cervical Exfoliated Cell's DNA Quantitative Analysis Based on Multi-Spectral Imaging Technology].

The conventional cervical cancer screening methods mainly include TBS (the bethesda system) classification method and cellular DNA quantitative analysis, however, by using multiple staining method in one cell slide, which is staining the cytoplasm with Papanicolaou reagent and the nucleus with Feulgen reagent, the study of achieving both two methods in the cervical cancer screening at the same time is still blank. Because the difficulty of this multiple staining method is that the absorbance of the non-DNA material may interfere with the absorbance of DNA, so that this paper has set up a multi-spectral imaging system, and established an absorbance unmixing model by using multiple linear regression method based on absorbance's linear superposition character, and successfully stripped out the absorbance of DNA to run the DNA quantitative analysis, and achieved the perfect combination of those two kinds of conventional screening method. Through a series of experiment we have proved that between the absorbance of DNA which is calculated by the absorbance unmixxing model and the absorbance of DNA which is measured there is no significant difference in statistics when the test level is 1%, also the result of actual application has shown that there is no intersection between the confidence interval of the DNA index of the tetraploid cells which are screened by using this paper's analysis method when the confidence level is 99% and the DNA index's judging interval of cancer cells, so that the accuracy and feasibility of the quantitative DNA analysis with multiple staining method expounded by this paper have been verified, therefore this analytical method has a broad application prospect and considerable market potential in early diagnosis of cervical cancer and other cancers.

[Biological Process Oriented Online Fourier Transform Infrared Spectrometer].

An online Fourier Transform Infrared Spectrometer and an ATR (Attenuated Total Reflection) probe, specifically at the application of real time measurement of the reaction substrate concentration in biological processes, were designed. (1) The spectrometer combined the theories of double cube-corner reflectors and flat mirror, which created a kind of high performance interferometer system. The light path folding way was utilized to makes the interferometer compact structure.

[Moving Mirror Scanning System Based on the Flexible Hinge Support].

In order to improve moving mirror drive of Fourier transform infrared spectrometer, we design a dynamic scanning system based on flexible hinge support. Using the flexible hinge support way and the voice coil motor drive mode. Specifically, Using right Angle with high accuracy high stability type flexible hinge support mechanism support moving mirror, dynamic mirror can be moved forward and backward driven by voice coil motor reciprocating motion, DSP control system to control the moving mirror at a constant speed.

[Hadamard Transform NIR Spectrometer for the Biological Processes].

Monitoring the consumption of nutrients of biological process helps control the growth environment of the microbes. It ensures the microbes are always in the best growing conditions, so as to maximize the yield of the target product. To monitor the content of glycerol, methanol and glucose in yeast culture medium, a new NIR spectrometer was developed which is based on Hadamard Transform (HT)technique.

The calibration of cellphone camera-based colorimetric sensor array and its application in the determination of glucose in urine.

In this work, a novel approach that can calibrate the colors obtained with a cellphone camera was proposed for the colorimetric sensor array. The variations of ambient light conditions, imaging positions and even cellphone brands could all be compensated via taking the black and white backgrounds of the sensor array as references, thereby yielding accurate measurements. The proposed calibration approach was successfully applied to the detection of glucose in urine by a colorimetric sensor array.

[AOTF-Based Imaging Spectrometer and Its Applications in the Identification of Metameric Targets].

For obtaining spectrum information and space distribution information of metameric targets at the same time, an AOTF-based imaging spectrometer was developed, which consists of a front imaging lens assembly, an AOTF filter imaging module, an AOTF driver, a plane array CCD, an image acquisition card and a PC. Under the control of the PC, a spectral image cube can be obtained by a fast wavelength scanning process, thereby the spectrum information of any point on the image is available. The developed imaging spectrometer, with a spectral range from 550-1,000 nm and a spectral resolution of 2.

[Development of a portable mid-infrared rapid analyzer for oil concentration in water based on MEMS linear sensor array].

Aiming at the existing problems such as weak environmental adaptability, low analytic efficiency and poor measuring repeatability in the traditional spectral oil analyzers, the present paper designed a portable mid-infrared rapid analyzer for oil concentration in water. To reduce the volume of the instrument, the non-symmetrical folding M-type Czerny-Turner optical structure was adopted in the core optical path. With a periodically rotating chopper, controlled by digital PID algorithm, applied for infrared light modulation, the modulating accuracy reached ±0.

[Design of a compact structure interferometer].

A novel interferometer system based on the combinations of cube-corner reflectors and fixed plane mirrors was designed, the moving mirror drive system was designed and analysed, and its governor PID algorithm was used to ensure that the movement of the moving mirror is collimated, uniform and smooth. The parameters of the optical system of the interferometer and the optical devices were described. Finally, after validation of the experiment, it was indicated that the wave number accuracy, resolution, signal to noise ratio and other key indicators can meet the needs of practical application.

[Design of a high-throughput and wide-bandwidth near-infrared acousto-optic tunable filter].

The acousto-optic tunable filter (AOTF) is the most outstanding progress of near-infrared spectrometer since the 1990s. Restriction in working range and performance of the device exists due to the traditional single-transducer structure. The design of a near-infrared acousto-optic tunable filter with high-throughput and wide-bandwidth adopting a dual-transducer structure is reported in the present paper.

[Testing method research for key performance indicator of imaging acousto-optic tunable filter (AOTF)].

Imaging AOTF is an important optical filter component for new spectral imaging instruments developed in recent years. The principle of imaging AOTF component was demonstrated, and a set of testing methods for some key performances were studied, such as diffraction efficiency, wavelength shift with temperature, homogeneity in space for diffraction efficiency, imaging shift, etc.

[A two-dimensional double dispersed hadamard transform spectrometer].

A kind of two-dimensional hadamard transform spectrometer was developed. A grating was used for chromatic dispersion of orders and a prism was used for spectral dispersion. Quite different from traditional CCD detection method, a digital micromirror device (DMD) was applied for optical modulation, and a simple point detector was used as the sensor.

[An interferometer design for Fourier transform infrared spectrometer].

A novel interferometer system based on the combinations of cube-corner reflectors and fixed plane mirrors was designed to improve the quality of spectra. It consists of a pair of cube corner reflectors, two fixed perpendicular plane mirrors and one beam splitter. By analyzing the modulation depth and phase error, the tilt and shearing of the cube corner reflectors, the deviation angle of cube corner reflector, the tilt of fixed plane mirrors and the deviation angle of two fixed plane mirrors were analyzed.

[A digital micromirror device-based Hadamard transform near infrared spectrometer].

A Hadamard transform near infrared spectrometer based on a digital micromirror device was constructed. The optical signal was collected by optical fiber, a grating was used for light diffraction, a digital micromirror device (DMD) was applied instead of traditional mechanical Hadamard masks for optical modulation, and an InGaAs near infrared detector was used as the optic sensor. The original spectrum was recovered by fast Hadamard transform algrithms.

Quantum dots-based immunofluorescence technology for the quantitative determination of HER2 expression in breast cancer.

HER2 detection is important for breast cancer (BC) treatment and prognosis, but the detection methods currently used have some disadvantages. Quantum dots (QDs)-based probes provide a potentially important new method for HER2 detection in clinical practice. This potential is examined in this paper.

Hadamard transform spectral microscopy for single cell imaging using organic and quantum dot fluorescent probes.

We report the development of Hadamard transform fluorescence spectral imaging microscopy by using a movable one-dimensional Hadamard mask to encode the image signals and a linear charge-coupled device (CCD) to detect the encoded spectral image signal. The technique has the ability of multi-spectral imaging and images with four dimensions: location coordinate (X and Y), fluorescence intensity (Z) and wavelength (lambda) (or time (t)) can be obtained. The results show that the system has high spectral resolving power (0.