Simultaneous determination of gallic acid, methyl gallate, and 1,3,6-tri-O-galloyl-β-d-glucose from Turkish galls in rat plasma using liquid chromatography-tandem mass spectrometry and its application to pharmacokinetics study.

Turkish galls (TG) is a traditional Uygur medicine typically used in clinics for dental disease and chronic ulcerative colitis. In this study, a novel liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification of gallic acid, methyl gallate, and 1,3,6-tri-O-galloyl-β-d-glucose in rat plasma, which are the major bioactive compounds of TG. After a feasible protein precipitation using acetonitrile for sample preparation, chromatographic separation was performed with a BDS Hypersil C18 column (2.

Evodiamine has therapeutic efficacy in ulcerative colitis by increasing Lactobacillus acidophilus levels and acetate production.

Emerging evidence implicates gut microbiota have an important role in ulcerative colitis (UC). Previous study indicated that Evodiamine (EVO) can alleviate colitis through downregulating inflammatory pathways. However, specific relationship between EVO-treated colitis relief and regulation of gut microbiota is still unclear.

[HPLC-UV fingerprints and chemical pattern recognition of Ilicis Pubescentis Radix].

The present study is to establish the fingerprints for the quality evaluation of Ilicis Pubescentis Radix by HPLC-UV. The chromatographic conditions were defined as Phenomenex Luna C₁₈(4.6 mm × 250 mm, 5 μm).

Simultaneous determination of borneol and its metabolite in rat plasma by GC-MS and its application to pharmacokinetic study.

A gas chromatography mass spectrometry (GC-MS) method has been developed and fully validated for the simultaneous determination of natural borneol (NB) and its metabolite, camphor, in rat plasma. Following a single liquid-liquid extraction, the analytes were separated using an HP-5MS capillary column (0.25 mm×30 m×0.

Simultaneous Quantification of Limonin, Two Indolequinazoline Alkaloids, and Four Quinolone Alkaloids in Evodia rutaecarpa (Juss.) Benth by HPLC-DAD Method.

A simple and efficient HPLC-DAD (225 nm) method was developed and validated for the simultaneous determination of limonin and six key alkaloids (evodiamine, rutaecarpine, 1-methyl-2-undecyl-4(1H)-quinolone, evocarpine, 1-methy-2-[(6Z,9Z)]-6,9-pentadecadienyl-4-(1H)-quinolone, and dihydroevocarpine) in Evodia rutaecarpa (Juss.) Benth, which has been widely used as one of the Traditional Chinese Medicines. The chromatographic separation was carried out on a Hypersil BDS C18 column, and gradient elution was employed with a mobile phase containing acetonitrile and water.

[Determination of apparent oil-water partition coefficient of andrographolide and dehydroandrographolide and effect of pH on them].

Objective: To determine the apparent n-octanol-water/buffer partition coefficient of andrographolide and dehydroandrographolide.

Methods: The apparent n-octanol-water/buffer partition coefficients of andrographolide and dehydroandrographolide were measured by shaking flask method. The concentrations of andrographolide and dehydroandrographolide were analyzed by HPLC method.

[Research on active part of Sambucus chinensis against hepatitis mice induced by CCl4].

Objective: To investigate the best extraction process of Sambucus chinensis against hepatitis and research on its active part.

Methods: We studied the protective effects of different extracts of Sambucus chinensis distilled by different extraction process on mice acute hepatic injury induced by CCl4, and searched for the active part of Sambucus chinensis against hepatitis from the best extract by extraction with different solvent and purification with macroporous adsorption resin, then studied their protective effects on mice acute hepatic injury induced by CCl4.

Results: The extraction of Sambucus chinensis by 75% alcohol showed very significantly protective effects on mice acute hepatic injury induced by CCl4 and the effects were better than that of other extraction process.

Gerardianin A, a new abietane diterpenoid from Isodon lophanthoides var. gerardianus.

A new abietane diterpenoid, gerardianin A (1), along with a known compound 6,7-dehydroroyleanone (2), has been isolated from the aerial parts of Isodon lophanthoides var. gerardianus [Bentham] H. Hara.

[Mass spectrometric analysis of quinazoline alkaloid and limonin from Fructus Evodiae].

Objective: To study Mass spectrometry of quinazoline alkaloid and limonin from Fructus Evodiae by an electrospray ionization (ESI) tandem mass spectrometry.

Methods: The ESI source was set at the positive ionization mode, and the MS1 and MS2 spectra of quinazoline alkaloid and limonin were acquired.

Results: The main fragments of quinazoline alkaloid and limonin from Fructus Evodiae were analyzed.

[Analysis of the main components of coptis-evodia herb couple by HPLC-DAD-MS].

A comprehensive HPLC-DAD-MS method was developed to study the chemical components of semi-bionic extract of Coptis-Evodia herb couple. The extract was isolated on a Hypersil BDS C18 column (4.6 mm x 200 mm, 5 microm) using acetonitrile-ammonium formic buffer as mobile phase by gradient elution.

Characterization of flavonoids in the extract of Sophora flavescens Ait. by high-performance liquid chromatography coupled with diode-array detector and electrospray ionization mass spectrometry.

A method coupling high-performance liquid chromatography (HPLC) with diode-array detector (DAD) and electrospray ionization mass spectrometry (ESI) was established for the separation and characterization of flavonoids in Sophora flavescens Ait. Based on the chromatographic separation of most flavonoids present in S. flavescens Ait.

[Study on the chemical constituents of Sambucus chinensis Lindl].

Objective: To study the chemical constituents of Sambucus chinensis Lindl.

Methods: The constituents were isolated and purified by various chromatographic methods and structurally identified by spectral analysis.

Results: 5 compounds were obtained as beta-sitosterol (I), oleanolic acid (II), ursolic acid (III), kaempferol-3-O-beta-D-(6-O-acetylglucopyranosid)-7-O-beta-D-glucopyranoside (IV), and kaempferol-3-O-beta-D-glucopyranosid-7-O-beta-D-glucopyranoside (V).

[Advances in research on chemistry and pharmacology of Sambucus chinensis].

The progress in research on the active ingredients of Sambucus Chinensis and pharmacological activities was reviewed. It is important to study the chemical constituents and phamacological activities of Sambucus chinensis.