Fast sequence alignment for centromere with RaMA.

The release of the first draft of the human pangenome has revolutionized genomic research by enabling access to complex regions like centromeres, composed of extra-long tandem repeats (ETRs). However, a significant gap remains as current methodologies are inadequate for producing sequence alignments that effectively capture genetic events within ETRs, highlighting a pressing need for improved alignment tools. Inspired by UniAligner, we develope Rare Match Aligner (RaMA), using rare matches as anchors and 2-piece affine gap cost to generate complete pairwise alignment that better capture genetic evolution.

FMAlign2: a novel fast multiple nucleotide sequence alignment method for ultralong datasets.

Motivation: In bioinformatics, multiple sequence alignment (MSA) is a crucial task. However, conventional methods often struggle with aligning ultralong sequences. To address this issue, researchers have designed MSA methods rooted in a vertical division strategy, which segments sequence data for parallel alignment.

Diagnosis of Small Bowel Obstruction using Targeted Enterography During Nasointestinal Decompression.

The aim of the study is to investigate the efficacy of using targeted enterography during intestinal decompression in the diagnosis of small bowel obstruction (SBO). Thirty-five patients with SBO and who had neither strangulation nor other contradictions received intestinal decompression, under the guidance of X-ray, using a 300-cm-long nasointestinal tube which reached the upper jejunum. Contrast radiography of intestines was performed when the tip of the decompression tube reached the obstruction by administering double-contrast medium, containing 20-100 ml 76 % gastrografin and 50-200 ml air, through the nasointestinal tube.