Toxicological evaluation of Chlamydomonas reinhardtii enriched with protoporphyrin IX.

The Chlamydomonas reinhardtii (red) is a strain rich in protoporphyrin IX, which could be used as algal-derived novel sustainable nutritional sources. However, the safety research on C. reinhardtii (red) remains insufficient and further investigation is needed.

Pyrroloquinoline quinone ameliorates renal fibrosis in diabetic nephropathy by inhibiting the pyroptosis pathway in C57BL/6 mice and human kidney 2 cells.

Diabetic nephropathy (DN), which is characterized by renal fibrosis, is a major complication of diabetes, a disease that afflicted more than 460 million people worldwide in 2019. Pyroptosis is an essential signaling pathway in DN-related injuries, such as renal fibrosis. Pyrroloquinoline quinone (PQQ) is a naturally occurring bioactive compound that protects human kidney 2 (HK-2) cells from oxidative stress-induced damage caused by high glucose concentrations.

Use of Luminex xMAP bead-based suspension array for detecting microRNA in NSCLC tissues and its clinical application.

Background: We measured the expression of microRNA (miRNA) in non-small cell lung cancer (NSCLC) tissues using the Luminex xMAP bead-based suspension array. We discuss the feasibility of employing this method to detect miRNA in NSCLC and explore its value as a high-throughput miRNA array.

Methods: We performed the methodological analysis of xMAP with oligoribonucleic acid references.

Application of a novel method PCR-ligase detection reaction for tracking predator-prey trophic links in insect-resistant GM rice ecosystem.

Insect-resistant genetically modified (IRGM) rice is on the verge of commercial release in China, however, its potential non-target effect on non-target insect natural enemies remains controversial. Tracking trophic interactions between predators and preys in IRGM rice ecosystem can provide new insights into better understanding of the ecological risks of IRGM rice. In the present study, a novel method based on ligase detection reaction (LDR), PCR-LDR was introduced to track 15 prey species in the gut of a predaceous spider Pirata subpiraticus, a dominant natural enemy in rice field.

[Development, optimization and application of the expression analysis platform based on multiplex quantitative RT-PCR using fluorescent universal primers].

A multiplex quantitative RT-PCR technology with a universal fluorescent primer was established. This technology employs a chimeric-primer-induced-universal-primer amplification method that ensures target genes amplified in a constant ratio. This technique was cost-effective, moderate-throughput, and reliable in quantification of gene expression.

Multiplex quantification of 16S rDNA of predominant bacteria group within human fecal samples by polymerase chain reaction--ligase detection reaction (PCR-LDR).

A new method, based on ligase detection reaction (LDR), was developed for quantitative detection of multiplex PCR amplicons of 16S rRNA genes present in complex mixtures (specifically feces). LDR has been widely used in single nucleotide polymorphism (SNP) assay but never applied for quantification of multiplex PCR products. This method employs one pair of DNA probes, one of which is labeled with fluorescence for signal capture, complementary to the target sequence.