Endoscopic type 1 tympanoplasty: comparison of the effects of three different thicknesses grafts.

Background: The effects of graft thickness on tympanoplasty is uncertain.

Objective: To compare the results of endoscopic tympanoplasty using different thicknesses of autologous tissues.

Methods: This retrospective analysis included 186 patients who received type I tympanoplasty, divided into three main groups based of grafting material: perichondrium (A), cartilage-perichondrium (B), or cartilage-perichondrium plus additional perichondrium (C).

Establishment of sperm associated antigen 6 gene knockout mouse model and its mechanism of deafness.

To investigate the effects of knocking out the Sperm associated antigen6 (Spag6) gene on the auditory system of mice, the heterozygous type Spag6 knockout mouse model built in the previous period was used for mating and breeding, and homozygous type Spag6 gene knockout mouse (Spag-/-), heterozygous type Spag6 gene knockout mouse (Spag+/-) and wild type mouse (Spag+/+) were obtained. PCR technology was used to verify mouse models with different genotypes. After verification, the hearing threshold responses of Spag+/+ and Spag-/- genotype mice were detected.