Identification of multiple miRNA-encoded peptide reveals OsmiPEP162a putatively stabilizes OsMIR162 in rice.

MiPEPs regulate growth, development and stress response. Identification of rice miPEPs plays a crucial role in elucidation of molecular functions of rice miPEPs and rice genetic improvement. MicroRNAs (miRNAs) are derivatives of primary miRNAs (pri-miRNAs) and govern the expression of target genes.

The novel cathelicidin-DM antimicrobial peptide conjugated carbomer and thermosensitive chitosan hydrogel speeds up wound-healing in both non-infected and S. aureus-infected wounds.

The emergence of chronic wound infections and bacterial resistance presents substantial clinical challenges that impact millions worldwide. Antimicrobial peptides (AMPs), recognized for their potent antimicrobial properties, are considered promising alternatives to conventional antibiotics in light of escalating drug resistance. In previous research, we isolated an AMP named cathelicidin-DM from Duttaphrynus melanostictus, which exhibited broad-spectrum efficacy against multidrug-resistant bacteria and demonstrated wound-healing capabilities.

A chemiluminescent sensor based on CRISPR-HCR technology for the hypersensitive detection of .

Tuberculosis is a highly infectious bacterial disease caused by . The spread of this agent has caused serious health problems worldwide, and the rapid and accurate detection of is essential for controlling the spread of infection and for preventing the emergence of multidrug-resistant strains. In this study, the cleavage ability of CRISPR-Cas12a against single-stranded DNA was combined with hybridization chain reaction and chemiluminescent signal to establish an imaging sensor for the hypersensitive detection of DNA.

An efficient CRISPR-Cas12a-mediated MicroRNA knockout strategy in plants.

In recent years, the CRISPR-Cas9 nuclease has been used to knock out MicroRNA (miRNA) genes in plants, greatly promoting the study of miRNA function. However, due to its propensity for generating small insertions and deletions, Cas9 is not well-suited for achieving a complete knockout of miRNA genes. By contrast, CRISPR-Cas12a nuclease generates larger deletions, which could significantly disrupt the secondary structure of pre-miRNA and prevent the production of mature miRNAs.

Raman-enhanced sensor based on CRISPR-SERS technology for the rapid and hypersensitive detection of Mycobacterium tuberculosis.

Tuberculosis is a highly infectious disease caused by the bacterium Mycobacterium tuberculosis, and the spread of this agent has caused serious health problems worldwide. The rapid and accurate detection of M. tuberculosis is essential for controlling the spread of infection and for preventing the emergence of multidrug-resistant strains.

Evaluation of the activity of antimicrobial peptides against bacterial vaginosis.

New drugs for the treatment of bacterial vaginosis (BV) are yet to be developed due to concerns that they may contribute to the increase in antibiotic resistance in BV. Antimicrobial peptides (AMPs) are one of the most promising options for next-generation antibiotics. In this study, we investigated the bacteriostatic activity of the AMPs Pexiganan, plectasin, melittin, and cathelicidin-DM against Gram-negative and Gram-positive bacteria both and in a mouse model of BV infection.

Cu,Zn,I-Doped Carbon Dots with Boosted Triple Antioxidant Nanozyme Activity for Treatment of DSS-Induced Colitis.

Nanozyme-mediated antioxidative therapy is a promising star for treating a myriad of important diseases through eliminating excessive reactive oxygen species (ROS) such as O and HO, a critical mechanism for inflammatory bowel disease (IBD). This work provides a high biocompatibility iodine-copper-zinc covalent doped carbon dots (Cu,Zn,I-CDs) with the catalase (CAT)-, superoxide dismutase (SOD)- and glutathione peroxidase (GPx)-like catalytic activities for treating ulcerative colitis (UC) by scavenging overproduced ROS. We found that I dopant aids in counteracting the positive charge at Cu,Zn dopants brought on by low pH, enabling Cu,Zn,I-CDs to process strong triple antioxidant nanozyme activities rather than Cu,Zn-CDs.

Boosting genome editing in plants with single transcript unit surrogate reporter systems.

CRISPR-Cas-based genome editing holds immense promise for advancing plant genomics and crop enhancement. However, the challenge of low editing activity complicates the identification of editing events. In this study, we introduce multiple single transcript unit surrogate reporter (STU-SR) systems to enhance the selection of genome-edited plants.

Exploration of the Antibacterial and Anti-Inflammatory Activity of a Novel Antimicrobial Peptide Brevinin-1BW.

Antibiotic resistance has emerged as a grave threat to global public health, leading to an increasing number of treatment failures. Antimicrobial peptides (AMPs) are widely regarded as potential substitutes for traditional antibiotics since they are less likely to induce resistance when used. A novel AMP named Brevinin-1BW (FLPLLAGLAASFLPTIFCKISRKC) was obtained by the Research Center of Molecular Medicine of Yunnan Province from the skin of the .

A fusion protein of vimentin with Fc fragment inhibits Japanese encephalitis virus replication.

Japanese encephalitis virus (JEV), a member of the Flaviviridae family and a flavivirus, is known to induce acute encephalitis. Vimentin protein has been identified as a potential receptor for JEV, engaging in interactions with the viral membrane protein. The Fc fragment, an integral constituent of immunoglobulins, plays a crucial role in antigen recognition by dendritic cells (DCs) or phagocytes, leading to subsequent antigen presentation, cytotoxicity, or phagocytosis.

Colorimetric assay for α-amanitin based on inhibition of carbon dots/AuNPs nanoenzyme activity.

Accidental ingestion of poisonous mushrooms leading to poisoning is a global issue. The most important and lethal toxin causing mushroom poisoning is α-amanitin, with a lethal dose of about 0.1 mg kg.

HBV Vaccines: Advances and Development.

Hepatitis B virus (HBV) infection is a global public health problem that is closely related to liver cirrhosis and hepatocellular carcinoma (HCC). The prevalence of acute and chronic HBV infection, liver cirrhosis, and HCC has significantly decreased as a result of the introduction of universal HBV vaccination programs. The first hepatitis B vaccine approved was developed by purifying the hepatitis B surface antigen (HBsAg) from the plasma of asymptomatic HBsAg carriers.

Enhancing diabetic wound healing with a pH-responsive nanozyme hydrogel featuring multi-enzyme-like activities and oxygen self-supply.

Diabetic wound treating remains a challenging due to bacterial infections, oxidative stress, tissue hypoxia, and high glucose levels. Herein, a multi-enzyme-like activities nanocomposite (Mo,Fe/Cu,I-Ag@GOx) was designed and anchored to a multifunctional fluorescence hydrogel. The nanozyme gel, loaded with glucose-oxidase (GOx), exhibits intrinsic GOx, peroxidase (POD)-, oxidase (OXD)-, catalase (CAT)- and superoxide dismutase (SOD)-like activities with pH-switchable glucose-initiated cascade reaction for diabetic wound healing.

A SYBR Green I-based aptasensor for the label-free, fluorometric, and anti-interference detection of MeHg.

Methylmercury (MeHg) is a common form of organic mercury that is substantially more toxic than inorganic mercury and is more likely to accumulate in organisms through biological enrichment. Therefore, developing a method to enable the specific and rapid detection of MeHg in seafood is important and remains challenging to accomplish. Herein, a rapid, label-free fluorescence detection method for MeHg determination was developed based on SYBR Green I.

Methylmercury-sensitized "turn on" SERS-active peroxidase-like activity of carbon dots/Au NPs nanozyme for selective detection of ochratoxin A in coffee.

The improvement and regulation of catalytic performance of nanozyme have long been pursued with sustained efforts. Herein, gold nanoparticles (S-CDs/AuNPs) with weak peroxidase-like (POD) activity were synthesized by Au-S bond using a sulfur doped carbon dots (S-CDs) as reducing agent and stabilizer. However, methylmercury (MeHg) could selectively and sensitively regulate the POD-like activity of S-CDs/AuNPs.

Transcriptomics based identification of S100A3 as the key anti-hepatitis B virus factor of 16F16.

More than 250 million people worldwide have chronic hepatitis B virus (HBV) infections, resulting in over 1 million annual fatalities because HBV cannot be adequately treated with current antivirals. Hepatocellular carcinoma (HCC) risk is elevated in the presence of the HBV. Novel and powerful medications that specifically target the persistent viral components are needed to remove infection.

An efficient CRISPR-Cas12a promoter editing system for crop improvement.

Promoter editing represents an innovative approach to introduce quantitative trait variation (QTV) in crops. However, an efficient promoter editing system for QTV needs to be established. Here we develop a CRISPR-Cas12a promoter editing (CAPE) system that combines a promoter key-region estimating model and an efficient CRISPR-Cas12a-based multiplexed or singular editing system.

A multi-enzyme-like activity exhibiting mussel-inspired nanozyme hydrogel for bacteria-infected wound healing.

Bacterial infection, tissue hypoxia, and inflammatory and oxidative stress are several key problems in wound healing of chronic infections. Herein, a multi-enzyme-like activity exhibiting multifunctional hydrogel made up of mussel-inspired carbon dot reduced-Ag (CDs/AgNPs) and Cu/Fe-nitrogen-doped carbon (Cu,Fe-NC) was designed. Due to the loss of glutathione (GSH) and oxidase (OXD)-like activity of the nanozyme (decomposes O to generate a superoxide anion radical (O˙) and hydroxyl radical production (˙OH)), the multifunctional hydrogel exhibited excellent antibacterial performance.

Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection.

A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins. Purification methods for recombinant proteins containing His-tags are relatively well-established, thus His-tags are widely used in protein recombination technology. We established a one-step enzyme-linked immunosorbent assay (ELISA) for His-tagged recombinant proteins.

Determination of Fumonisin B by Aptamer-Based Fluorescence Resonance Energy Transfer.

Fumonisin FB is produced by Sheld, of which FB is the most common and the most toxic. The establishment of a rapid detection method is an important means to prevent and control FB pollution. A highly sensitive fluorescent sensor based on an aptamer for the rapid detection of fumonisin B (FB) in corn was established.

Wound healing mechanism of antimicrobial peptide cathelicidin-DM.

Chronic wound infections and the development of antibiotic resistance are serious clinical problems that affect millions of people worldwide. Cathelicidin-DM, an antimicrobial peptide from , has powerful antimicrobial activity and wound healing efficacy. So, it could be a potential candidate to address this problem.

Establishment of fumonisin B detection method for catalytic fluorescence detection of aptamer-regulated carbon dots.

Mycotoxin, common in agricultural products, is a small secondary metabolite with strong toxicity. Fumonisin B (FB) is the most common and the most toxic. Establishing a rapid detection method is important for preventing and controlling FB pollution.