Development and evaluation of puerarin-loaded controlled release nanostructured lipid carries by central composite design.

The present work was aimed at developing optimized puerarin-loaded nanostructured lipid carrier (PA-NLC) on base of phospholipid complex. The puerarin phospholipid complex (PA-PC) was prepared by a solvent evaporation method and the formulation was confirmed according to the encapsulation efficiency (EE%). The hepatoprotective effect of PA-NLC on BRL 3A cell stimulated by ethanol was carried out using MTT assay, and cell imaging was done using an inverted phase contrast tissue culture microscope.

Development and evaluation of lutein-loaded alginate microspheres with improved stability and antioxidant.

Background: Lutein has been of great interest to the food processing and pharmaconutrient industries owing to its beneficial effects on human health. However, lutein is very sensitive to heat, light, pH and oxidative conditions, which limits its application in food systems. The present study aimed to prepare lutein-alginate microspheres by a calcium chloride gelation method with the purpose of improving the stability and antioxidant abilities of lutein.