Creation of a Plant Metabolite Spectral Library for Untargeted and Targeted Metabolomics.

Large-scale high throughput metabolomic technologies are indispensable components of systems biology in terms of discovering and defining the metabolite parts of the system. However, the lack of a plant metabolite spectral library limits the metabolite identification of plant metabolomic studies. Here, we have created a plant metabolite spectral library using 544 authentic standards, which increased the efficiency of identification for untargeted metabolomic studies.

Differential tolerance to heat stress of young leaves compared to mature leaves of whole plants relate to differential transcriptomes involved in metabolic adaptations to stress.

Plants respond to heat shock by regulating gene expression. While transcriptomic changes in response to heat stress are well studied, it is not known whether young and old leaves reprogram transcription differently upon stress. When whole plants of were subjected to heat shock, young leaves were affected significantly less than older leaves based on measurements of tissue damage.

Advances and perspectives in the metabolomics of stomatal movement and the disease triangle.

Crops are continuously exposed to microbial pathogens that cause tremendous yield losses worldwide. Stomatal pores formed by pairs of specialized guard cells in the leaf epidermis represent a major route of pathogen entry. Guard cells have an essential role as a first line of defense against pathogens.

New aspects of iron-copper crosstalk uncovered by transcriptomic characterization of Col-0 and the copper uptake mutant spl7 in Arabidopsis thaliana.

Iron (Fe) and copper (Cu) are essential micronutrients for energy metabolism and reactive oxygen species (ROS) scavenging. Some Cu-containing proteins can be substituted with Fe-containing proteins, and vice versa, while several Arabidopsis genes are regulated by both metals. Few details of how plants coordinate Fe-Cu crosstalk are known.