Publications by authors named "Qingwen Zhi"
[Cloning, expression and evaluation of Saccharomyces cerevisiae ADH2].
Sheng Wu Gong Cheng Xue Bao
February 2010
Article Synopsis
- Researchers cloned and expressed the ADH2 gene from the yeast Saccharomyces cerevisiae in E. coli BL21 (DE3) using total RNA and RT-PCR to create a recombinant plasmid.
- The recombinant plasmid pTAT-ADH2 was transformed into E. coli and induced with IPTG to produce the ADH2 enzyme, which was then purified and tested for activity.
- The sequence analysis showed 90% homology with the GenBank report, and the expressed ADH2 demonstrated significant ethanol dehydrogenase activity in biochemical and mouse toxicological tests.
[PTD-hEGF fusion protein--gene expression and function analysis].
Sheng Wu Gong Cheng Xue Bao
July 2007
To produce membrane-permeable human epidermal growth factor (hEGF), a pPTD-hEGF prokaryocyte expression vector was constructed and transformed into E. coli BL 21 (DE3). The PTD-hEGF fusion protein was induced by 0.
View Article and Find Full Text PDFTransgenic mini-tomato and protection against alcohol-induced gastric injury.
J Genet Genomics
August 2007
The epidermal growth factor (EGF) has been shown to promote the proliferation of various types of cells, to maintain the physiological function of the mucosa of the digestive tract, and to promote the healing of the gastric and duodenal ulcers. It has been expressed in many types of bacteria and yeasts. In this article, a bio-reactor was constructed, namely, the human EGF (hEGF) transgenic mini-tomato.
View Article and Find Full Text PDF[Generation and identification of rice T-DNA insertional mutant lines].
Yi Chuan Xue Bao
December 2004
By using the matured embryos of Japonica rice variety Zhonghua No. 11 as explants, rice transformation was performed by Agrobacterium-mediated co-cultivation method, resulting in 1489 independent transgenic rice plants that carry a T-DNA insertion. Genomic DNA gel-blot and PCR analyses showed that 69.
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