Humanized monoclonal antibody against the chemokine CXCL-8 (IL-8) effectively prevents acute lung injury.

As one of the most important endogenous chemotactic factors for neutrophils, the chemokine CXCL8 (IL-8) is involved in the pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), characterized by massive neutrophil infiltration in the lung. Since neutralization of CXCL8 with polyclonal antibody has been shown to reduce the severity of ALI/ARDS in animal models, we explored the potential of humanized anti-CXCL8 antibody as a preventive or therapeutic agent for ALI. We used a 'two-hit' protocol to induce ALI in rabbits that showed extensive edema in the alveolar lumina, marked infiltration of neutrophils in the lung tissue, fibrin deposition in alveolar space, and destruction of pulmonary architecture, culminating in severe hypoxemia.

[Detection of interleukin-8 on tissue array of non-small cell lung cancer and its clinicopathological significance].

Background: Interleukin-8 (IL-8) is a member of the CXC chemokine family, which involved in tumor growth, metastasis and angiogenesis. The aim of this study is to investigate the expression of IL-8 in non-small cell lung cancer (NSCLC) and its clinicopathologic significance.

Methods: The expression of IL-8 protein and the intratumoral microvessel density (MVD) were detected in 114 cases of NSCLC on tissue array by immunohistochemical method (EnVisionTM method).