Isolation, characterization, and genomic analysis of a lytic bacteriophage, PQ43W, with the potential of controlling bacterial wilt.

Bacterial wilt (BW) is a devastating plant disease caused by the soil-borne bacterium species complex (Rssc). Numerous efforts have been exerted to control BW, but effective, economical, and environmentally friendly approaches are still not available. Bacteriophages are a promising resource for the control of bacterial diseases, including BW.

sp. nov., an oligotrophic, slightly acidophilic mesophile isolated from sandy soil.

A bacterial strain, designated S6, was isolated from the sandy soil on a rocky mountain in South China. Cells of S6 were Gram-stain-negative, aerobic, non-spore-forming, non-motile and non-prosthecae-producing. 16S rRNA gene sequence analysis revealed the highest similarities to 12 uncultured bacteria, followed by sp.

First report of causing southern blight on cowpea in China.

Cowpea ( L.) is a legume consumed as a high-quality plant protein source in many parts of the world. In August 2023, it was observed that a plant disease affected cowpea growth in Yiyang (28.

Polyamine-containing natural products: structure, bioactivity, and biosynthesis.

Covering: 2005 to August, 2023Polyamine-containing natural products (NPs) have been isolated from a wide range of terrestrial and marine organisms and most of them exhibit remarkable and diverse activities, including antimicrobial, antiprotozoal, antiangiogenic, antitumor, antiviral, iron-chelating, anti-depressive, anti-inflammatory, insecticidal, antiobesity, and antioxidant properties. Their extraordinary activities and potential applications in human health and agriculture attract increasing numbers of studies on polyamine-containing NPs. In this review, we summarized the source, structure, classification, bioactivities and biosynthesis of polyamine-containing NPs, focusing on the biosynthetic mechanism of polyamine itself and representative polyamine alkaloids, polyamine-containing siderophores with catechol/hydroxamate/hydroxycarboxylate groups, nonribosomal peptide-(polyketide)-polyamine (NRP-(PK)-PA), and NRP-PK-long chain poly-fatty amine (lcPFAN) hybrid molecules.

First Report of Southern Blight Caused by on Pepper in Yiyang, China.

Pepper( L.) is the vegetable with the largest production area China (Zou and Zou 2021). In the summer of 2020 and 2021, disease symptoms were observed in L.

A recombineering system for based on the native phage recombinase pair YqaJ/YqaK.

plays an important role in fundamental and applied research, and it has been widely used as a cell factory for the production of enzymes, antimicrobial materials, and chemicals for agriculture, medicine, and industry. However, genetic manipulation tools for have low efficiency. In this work, our goal was to develop a simple recombineering system for .

Characterization of a pathway-specific activator of edeine biosynthesis and improved edeine production by its overexpression in .

Edeines are a group of non-ribosomal antibacterial peptides produced by . Due to the significant antibacterial properties of edeines, increasing edeine yield is of great interest in biomedical research. Herein, we identified that EdeB, a member of the ParB protein family, significantly improved edeine production in .

Optimization, Characteristics, and Functions of Alkaline Phosphatase From .

Weaning of piglets could increase the risk of infecting with Gram-negative pathogens, which can further bring about a wide array of virulence factors including the endotoxin lipopolysaccharide (LPS). It is in common practice that the use of antibiotics has been restricted in animal husbandry. Alkaline phosphatase (AKP) plays an important role in the detoxification and anti-inflammatory effects of LPS.

The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts.

Phytopathogenic fungi infect crops, presenting a worldwide threat to agriculture. Polyene macrolides are one of the most effective antifungal agents applied in human therapy and crop protection. In this study, we found a cryptic polyene biosynthetic gene cluster in by genome mining.

Complete Genome Sequence of sp. Strain S6, Obtained Using Oxford Nanopore and Illumina Sequencing.

We report the complete genome sequence of sp. strain S6, generated from Oxford Nanopore and Illumina sequencing. The assembled genome comprises a chromosome with a length of 5.

Enhancement of edeine production in Brevibacillus brevis X23 via in situ promoter engineering.

Edeines, a group of cationic antimicrobial peptides produced by the soil bacterium Brevibacillus, have broad biological effects, such as antimicrobial, anticancer and immunosuppressive activities. However, the yield of edeines in wild-type (WT) Brevibacillus is extremely low, and chemical synthesis of edeines is a time-consuming process. Genetic engineering has proven to be an effective approach to produce antibiotics with high yield.

Complete Genome Sequence of sp. Strain M6, Isolated from a Rocky Mountain in China.

We report the complete genome sequence of sp. strain M6 obtained by Oxford Nanopore and Illumina sequencing. The approximately 3.

An automatic segmentation method for heart sounds.

Background: There are two major challenges in automated heart sound analysis: segmentation and classification. An efficient segmentation is capable of providing valuable diagnostic information of patients. In addition, it is crucial for some feature-extraction based classification methods.

Environmental factors shaping the diversity of bacterial communities that promote rice production.

Background: Exploiting soil microorganisms in the rhizosphere of plants can significantly improve agricultural productivity; however, the mechanism by which microorganisms specifically affect agricultural productivity is poorly understood. To clarify this uncertainly, the rhizospheric microbial communities of super rice plants at various growth stages were analysed using 16S rRNA high-throughput gene sequencing; microbial communities were then related to soil properties and rice productivity.

Results: The rhizospheric bacterial communities were characterized by the phyla Proteobacteria, Acidobacteria, Chloroflexi, and Verrucomicrobia during all stages of rice growth.

Yield improvement of epothilones in Burkholderia strain DSM7029 via transporter engineering.

Transporter engineering has been shown to be a positive approach for enhancing natural product titers in microbial cell factories by expelling target compounds out of feasible hosts. In this work, two multidrug efflux pumps, Orf14 and Orf3, were modulated in the epothilone production strain Burkholderia DSM7029::Tn5-km-epo (named G32) via Red/ET engineering to increase heterologous polyketide epothilone yields. Compared with the prior G32 strain, the total production of several epothilones in the G32::orf14-orf3 mutant was meaningfully doubled according to high-performance liquid chromatography-mass spectrometer analysis.

Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster in Bacillus subtilis via Red/ET recombineering.

Heterologous expression of biosynthetic pathways is an important way to research and discover microbial natural products. Bacillus subtilis is a suitable host for the heterologous production of natural products from bacilli and related Firmicutes. Existing technologies for heterologous expression of large biosynthetic gene clusters in B.

Decolorization and degradation of reactive dye during the dyed cotton fabric rinsing process.

Dyeing process of textile consumes large quantities of water, which results in huge amounts of colored wastewater. Most of the dye wastewater treating methods focused on the treatment of wastewater after the rinsing process of dyed textile. In this paper, tetraacetylethylenediamine/hydrogen peroxide (TAED/H₂O₂) active oxidation (AO) system was developed to rinse dyed textile and decolorize the rinsing wastewater simultaneously.