Bronchiolitis obliterans complicating a pneumothorax after Stevens-Johnson syndrome induced by lamotrigine.

Bronchiolitis obliterans (BO) was defined as a nonreversible obstructive lung disease in which the bronchioles are always compressed and narrowed by fibrosis or inflammation. In the severe event of lung collapse after BO, surgical intervention is often recommended, and conservative therapy is thought to be ineffective. Here, we report the case of a 9-year old girl clinically diagnosed as having bronchiolitis obliterans with abrupt occlusion of the right B4b bronchus.

Effects of lipid emulsions in parenteral nutrition of esophageal cancer surgical patients receiving enteral nutrition: a comparative analysis.

Background: Olive oil-based lipid emulsion (LE) and medium chain triglyceride/long chain triglyceride (MCT/LCT) emulsion are both LEs with low ω-6 polyunsaturated fat acids (PUFAs) content. However, which one of these LEs is associated with a lower infection risk in patients receiving parenteral nutrition (PN) remains unclear. The aim of the study was to compare the effects of the two LEs in PN in esophageal cancer patients undergoing surgery.

Isolated Crohn's disease of the esophagus with esophago-mediastinal fistula formation.

Isolated Crohn's disease of the esophagus is rare, and accurate diagnosis and treatment in its early course are difficult. Most cases are often found very late, when severe strictures or other complications have occurred. We report the case of a male 60-year-old patient with complaints of progressive dysphagia for more than two months and the sudden appearance of heartburn for seven consecutive days.

A case report of para-esophageal bronchogenic cyst with esophageal communication.

Paraesophageal bronchogenic cyst was one of common mediastinal congenital cystic lesions of foregut origin. Because of an intimate embryologic relationship with the esophagus, they were usually found intramural (intramural esophageal bronchogenic cysts) with the local esophageal mucosa being intact and the paraesophageal bronchogenic cysts were rarely communicated with esophageal lumen. We report a case of para-esophageal bronchogenic cyst communicating to the esophageal lumen thorough a pedicle of canal, which looked liked a diverticulum on X-ray.

CT-guided percutaneous microwave ablation of pulmonary malignancies: Results in 69 cases.

Background: Microwave ablation (MWA) has attracted a worldwide attention gradually in treating inoperable pulmonary malignancies. However, in the lung tissues treated with MWA recurrence of tumor may still occur and few data in large patient groups till now were reported about the safety or effectiveness of microwave ablation in treating primary lung cancer and metastatic pulmonary malignancies. The purpose of this study is to evaluate the clinical curative effect (local control, survival data) MWA and its safety as well.

[Expression and its clinical significance of SLC22A18 in non-small cell lung cancer].

Background And Objective: It has been proven that multidrug resistance (MDR) is the main cause of chemotherapy failure in lung cancer. Research on emergence mechanisms of MDR has great clinical significance in improving the curative efficiency of lung cancer chemotherapy. Proteins encoded by the SLC22A18 gene, which is similar to the transmembrane transporter, may influence the sensitivity of chemotherapeutics as well as the metabolism and growth of cells.

[Experimental study on acute respiratory distress syndrome and analysis of relevant factors in rabbits subjected to thoracic blast trauma].

Objective: To reproduce acute respiratory distress syndrome (ARDS) model in rabbit induced by chest blast injury and to analyze the pathogenesis and causes of early death in order to provide the basis for the early diagnosis of lung blast injury and its early warning system to facilitate an early treatment.

Methods: Sixty healthy New Zealand white rabbits were divided into six groups according to the different explosion distance with the random number table method. The survival rate and its resulting pathological changes were observed and patho physiological indexes and lung fluid content were determined at sequential time points post explosion.

Is radiofrequency thermal ablation a safe and effective procedure in the treatment of pulmonary malignancies?

Objective: Radiofrequency ablation (RFA) has been recently applied as an alternative option of pulmonary surgery in the treatment of pulmonary malignancies. In this study, we assessed the risk associated with percutaneous RFA, and discussed its safety and efficacy.

Methods: The clinical data of 329 consecutive patients with primary (n = 237) and metastatic (n = 92) lung tumor treated with RFA from 1999 to 2006 in this hospital were considered for this study, and the character and clinical data of these patients were analyzed.

Repair of massive stent-induced tracheoesophageal fistula.

Objective: The purpose of this report was to discuss a new surgical procedure in treating esophageal stent related large tracheoesophageal fistula without tracheal resection.

Methods: Clinical records of 5 patients with esophageal stent-related large tracheoesophageal fistulas treated in this hospital between 1997 and 2006 were reviewed.

Result: All patients had insertion of a covered self-expanding esophageal stent, 1 for benign esophageal stricture and 4 for esophageal perforation resulting from various causes.

Bone morphogenetic protein-2 stimulation of cartilage regeneration in canine tracheal graft.

Background: Graft stenosis is among the most serious post-surgical complications that can occur after tracheal transplantation. Typically, stenosis is caused by resorption of tracheal cartilage. Bone morphogenetic protein-2 (BMP-2) is efficient at stimulating bone or cartilage regeneration.

[The expression of ABCG4, V-ATPase and clinic significance of their correlation with NSCLC.].

Background: It has been proven that the multiple drug resistance is main reason for failure of chemotherapy in lung cancers and ABC transporter play a main role for chemoresistance in mediating drug efflux. So searching for new drug resistant protein of the ABC family and elucidating its resistant mechanism is very important. ABCG4 is one of ABC family and is expected to be candidate drug resistant protein; and the drug resistance probably correlated with pH value around cancer cell, while, V-ATPase play key role in modulating the pH.

Enhancement of radiosensitivity by roscovitine pretreatment in human non-small cell lung cancer A549 cells.

Roscovitine has been reported to have anti-proliferative properties and is in process of undergoing clinical trials. In addition to its intrinsic anticancer properties, it has recently been suggested that roscovitine may also enhance the activity of traditional chemo- and radio- therapies in certain cancer cell lines. The purpose of this study was to define the activity of roscovitine in increasing radiosensitivity of human non-small cell lung cancer (NSCLC) cell line A549 cells in vitro.

Lung cancer A549 cells migrate directionally in DC electric fields with polarized and activated EGFRs.

Endogenous direct-current electric fields (dcEFs) occur in vivo in the form of epithelial transcellular potentials or neuronal field potentials. A variety of cells respond to dcEFs by migrating directionally, and this is termed galvanotaxis. The mechanism by which dcEFs direct cell movement, however, is not yet understood, and the effects on lung cancer cells are entirely unknown.

[c-fos antisense oligodeoxynucleotide reduces VIP-induced upregulation of VEGF expression in small cell lung cancer cells].

Background: It has been known that vasoactive intestinal peptide (VIP) has the effect of promoting the growth of some malignant tumors, but its mechanism is not clear. The aim of this study is to use c-fos antisense oligodeoxynucleotide (ASO) to block c-fos expression and to explore whether c-fos can directly regulate VIP-induced VEGF expression in small cell lung cancer (SCLC) cells.

Methods: Expression levels of c-fos and VEGF genes were detected in SCLC cell line H446 treated with VIP by RT-PCR.

[The initial value evaluation of ¹⁸F-fluorodeoxyglucose positron emission tomography in the diagnosis of chest malignant tumors].

Background: ¹⁸F-fluorodeoxyglucose positron emission tomography (¹⁸F-FDG PET) is considered as one of the most valuable diagnostic methods of chest malignant tumors, except for pathological diagnosis. But in clinic, the cases whose diagnosis of ¹⁸F-FDG PET is different from pathological diagnosis are not rare. This study is to evaluate the initial value of ¹⁸F-FDG PET in the diagnosis and staging of the chest malignant tumors.

A rat surgical model of esophageal metaplasia and adenocarcinoma-induced by mixed reflux of gastric acid and duodenal contents.

Recent clinical data have revealed that mixed reflux (MR) of gastric acid and duodenal contents frequently occurs in patients with gastroesophageal reflux disease and a progressive increase of MR occurs with increasing severity across gastroesophageal reflux disease. Herein we report a novel rat surgical model in which esophageal metaplasia and adenocarcinoma develop as complications of MR. The model was created by performing an esophagojejunostomy and a gastrojejunostomy 5 mm proximal to the esophagojejunal anastomosis in 40 rats.

[Association of MMP1 -1607(1G>2G)single nucleotide polymorphism with susceptibility to lung cancer in Northwestern Chinese population of Han nationality].

Objective: Matrix metalloproteinase 1 (MMP1) plays an important role in the development of lung cancer. This study was to investigate the relation to associate the single nucleotide polymorphism(SNP)in MMP1 gene with the susceptibility to lung cancer in Northwestern Chinese population of Han nationality.

Methods: By using the methods of polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP), MMP1 -1607(1G>2G) polymorphisms in 150 patients with lung cancer, and 200 healthy controls were detected to evaluate the relationship between different genotypes and susceptibility of lung cancer.

[Vasoactive intestinal peptide enhances the expression of vascular endothelial growth factor mRNA in non-small cell lung cancer cells].

Background: Some researches have found that the development of tumor could be encouraged by vascular endothelial growth factor (VEGF) and vasoactive intestinal peptide (VIP), but how about the mode of VIP? The aim of this study is to examine the effects of VIP on expression of VEGF mRNA in non-small cell lung cancer (NSCLC) cells.

Methods: Expression of VEGF mRNA was detected in NSCLC and small cell lung cancer (SCLC) cell lines by reverse transcriptase-polymerase chain reaction (RT-PCR) technique.

Results: VEGF mRNA was detected in NSCLC cell lines A549, GLC-82, H157, H460 and SCLC cell line H446.

Surgical management of esophageal strictures after caustic burns: a 30 years of experience.

Aim: To analyze a 30-year historical series of patients treated in our hospital, who ingested corrosive substances, and to assess the effectiveness of surgical therapy administered in patients with strictures after caustic injury in esophagus during this period.

Methods: A total of 79 cases of caustic burns in esophagus were treated in Tangdu Hospital from 1971 to 2001. Their clinical and pathological data were reviewed, and collected from the medical records of patients and interviews with them.

[Experimental study on the effect of VEGF165 antisense RNA on human squamous cell carcinoma of esophagus].

Aim: To investigate the effect of antisense RNA against vascular endothelial growth factor 165 (VEGF165) on human esophagus squamous cell carcinoma cell line EC109.

Methods: Eukaryotic expression vector for VEGF165 antisense RNA was constructed and identified. Recombinant plasmid was transfected into EC109 cells and the transfected EC109 cells were inoculated subcutaneously to nude mice.

[Effect of anti-VEGF₁₆₅ ribozyme on biological behaviors of human lung adenocarcinoma cell].

Background: To investigate the biological effects of anti VEGF₁₆₅ ribozyme on human lung adenocarcinoma cell.

Methods: Hammerhead ribozyme (VRz) against VEGF₁₆₅ gene transcripts (site 212) and its paired mutant ribozyme (mVRz) were designed and synthesized, and the cleavage activity of the ribozymes on target RNA in a cell-free system was observed. The replication-incompetent adenovirus-mediated eukaryotic expression vectors (rpAdVRz) containing VRz and mVRz gene were constructed and identified.

[Experimental study of canine tracheal allotransplantation].

Objective: To detect the factors relevant to stenosis of tracheal graft and to find feasible methods to solve this problem.

Methods: Sixteen mongrel dogs were divided into groups A and B randomly and equally. Five-ring-length tracheal segments were allotransplanted.

[The biological effects of multi-electrode radiofrequency ablation on normal rabbit lungs].

Background: To study the biological effects of multi-electrode radiofrequency ablation on pulmonary tissues of rabbits.

Methods: Under the guidance of computer tomograph, electrodes were inserted into right lungs of New Zealand white rabbits and radiofrequency was performed. The biological effects were observed through CT image and microscopy.

[Effect of transfection of p27(kipl) on lung cancer cells line A549].

Background: To observe the suppressive effects of exogenous p27 gene on human lung cancer cell line A549.

Methods: An adenovirus expression vector (pAd CMV-p27) containing 570 bp human full-length p27 cDNA was transfected into human lung cancer cell line A549. Expression of exogenous p27 gene was detected by dot-blot hybridization and laser co-focal system.