Simultaneous detection of mixed colorants adulterated in black tea based on various morphological SERS sensors.

Colorant adulteration is a common problem in tea safety control; thus, a rapid identification method is required. In this study, we optimized the fabrication parameters of various sensors to enhance their performance. R6G was used as a probe molecule, demonstrating that the sensnor remained stable for 120 days.

E-nose and colorimetric sensor array combining homologous data fusion strategy discriminating the roasting degree of large-leaf yellow tea.

Different degrees of roasting result in differences in the quality and flavor of large-leaf yellow tea. The current sensory evaluation and chemical detection methods cannot meet the requirement of online differentiation of LYT roasting degree, so an accurate and comprehensive assessment method needs to be developed urgently. First, the two aroma sensing technologies were compared.

Rapid and comprehensive grade evaluation of Keemun black tea using efficient multidimensional data fusion.

To develop a comprehensive evaluation method for Keemun black tea, we used micro-near-infrared spectroscopy, computer vision, and colorimetric sensor array to collect data. We used support vector machine, least-squares support vector machine (LS-SVM), extreme learning machine, and partial least squares discriminant analysis algorithms to qualitatively discriminate between different grades of tea. Our results indicated that the LS-SVM model with mid-level data fusion attained an accuracy of 98.

Multi-Ce-Inserted Phospho(III)tungstate Containing Three Building Units with Prominent Peroxidase-Mimicking Activity for Detecting l-Cysteine.

An attractive isonicotinic acid-ornamented octa-Ce-inserted phospho(III)tungstate [HN(CH)]Na[Ce(HO)WNaO(INA)][HPWO][HPWO]·30HO () (HINA = isonicotinic acid) has been isolated through the deliberately designed one-step assembly strategy, in which the HPO heteroanion template was introduced into the Ce/WO system in the presence of HINA. The polyoxoanion of consists of two identical [Ce(HO)WNaO(INA)][HPWO][HPWO]} subunits linked by Ce-O-W bonds. The polyoxoanion exhibits three kinds of polyoxotungstate building blocks [WNaO(INA)], [HPWO], and [HPWO], in which [WNaO(INA)] and [HPWO] building units can be considered as seeds driven by the coordination of additional Ce ions to induce aggregation of [HPWO] fragments.

A rapid aroma quantification method: Colorimetric sensor-coupled multidimensional spectroscopy applied to black tea aroma.

Aroma affects the quality of black tea, and the rapid evaluation of aroma quality is the key to realize the intelligent processing of black tea. A simple colorimetric sensor array coupled with a hyperspectral system was proposed for the rapid quantitative detection of key volatile organic compounds (VOCs) in black tea. Feature variables were screened based on competitive adaptive reweighted sampling (CARS).

Evaluation of the effects of solar withering on nonvolatile compounds in white tea through metabolomics and transcriptomics.

The mechanism through which solar withering (SW) affects the quality of white tea is unclear. To address this gap in the literature, in this study, we used metabolomics and transcriptomics to investigate the effect of SW on the quality of WT. WT that underwent SW was slightly more bitter and astringent than WT that underwent natural withering (control group).

Rapid detection of multiple colorant adulteration in Keemun black tea based on hemp spherical AgNPs-SERS.

This study synthesized stable and sensitive hemp spherical AgNPs as the SERS substrate for the simultaneous and rapid detection of sunset yellow, lemon yellow, carmine and erythrosine adulteration in black tea. With R6G as the probe molecule, the AgNPs were determined to have satisfactory stability over 60 days with an enhancement factor of 10. The effects of three variable screening methods on model performance were compared.

Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes.

Many plants can successfully join root and shoot sections at cut surfaces when severed at the stem. Graft healing is complex and conserved in diverse taxonomic groups with different vascular structures. Herein, we compared transcriptome data from autografted and separated stem sections of and tomato () to explore changes related to graft healing.

Rapid monitoring of black tea fermentation quality based on a solution-phase sensor array combined with UV-visible spectroscopy.

Rapid monitoring of fermentation quality has been the key to realizing the intelligent processing of black tea. In our study, mixing ratios, sensing array components and reaction times were optimized before an optimal solution phase colorimetric sensor array was constructed. The characteristic spectral information of the array was obtained by UV-visible spectroscopy and subsequently combined with machine learning algorithms to construct a black tea fermentation quality evaluation model.

Rapid identification of the green tea geographical origin and processing month based on near-infrared hyperspectral imaging combined with chemometrics.

The geographical origin and processing month of green tea greatly affect its economic value and consumer acceptance. This study investigated the feasibility of combining near-infrared hyperspectral imaging (NIR-HSI) with chemometrics for the identification of green tea. Tea samples produced in three regions of Chongqing (southeastern Chongqing, northeastern Chongqing, and western Chongqing) for four months (from May to August 2020) were collected.

KAT1 triggers YTHDF2-mediated ITGB1 mRNA instability to alleviate the progression of diabetic retinopathy.

Diabetic retinopathy (DR) is a major complication of diabetes and a leading cause of blindness and visual impairment. This study focuses on the function of lysine acetyltransferase 1 (KAT1) in the progression of DR and the epigenetic mechanism. A mouse model with DR was induced by streptozotocin (STZ).

Stage-specific events in tomato graft formation and the regulatory effects of auxin and cytokinin.

Grafting is widely used worldwide because of its obvious advantages, especially in solanaceous vegetable crops. However, the molecular mechanisms underlying graft formation are unknown. In this study, internode tissues from above and below the graft junction were harvested, and we performed weighted gene co-expression network analysis (WGCNA) to describe the temporal and spatial transcriptional dynamics that occur during graft formation in tomato.

Knockdown of Long Non-Coding RNA LOC100132707 Inhibits the Migration of Uveal Melanoma Cells via Silencing JAK2.

Background/objective: Although lots of long non-coding RNAs (lncRNAs) have been demonstrated to be involved in carcinogenesis, the functions of numerous of lncRNAs remain unknown. Bioinformatics online database showed that lncRNA LOC100132707 was highly expressed in metastatic melanoma tissues, and its expression predicted a lower overall survival rate in melanoma patients. However, LOC100132707 function in uveal melanoma (UM) progression still remains unclear.

Long Non-Coding RNA GAS5 Targeting microRNA-21 to Suppress the Invasion and Epithelial-Mesenchymal Transition of Uveal Melanoma.

Objective: Human uveal melanoma (UM) is a common ocular malignant tumor with a high risk of metastasis. Emerging evidence indicates that long non-coding RNAs (lncRNAs) are correlated with the development of UM. Here, we aimed to determine the biological significance of lncRNA growth arrest-specific transcript 5 (GAS5) in UM.

Carriage of Clostridioides difficile in healthy infants in the community of Handan, China: A 1-year follow-up study.

Objective: Clostridioides difficile may colonize healthy infants and young children asymptomatically and for the long-term. C. difficile genotypes and the rate and determinants of colonization differ substantially and vary among countries and regions.

Physiological and iTRAQ based proteomics analyses reveal the mechanism of elevated CO concentration alleviating drought stress in cucumber (Cucumis sativus L.) seedlings.

Carbon dioxide is one of the most important anthropogenic greenhouse gases. We previously confirmed that elevated [CO] alleviated the negative consequences of drought stress to cucumber seedlings, but the physiological mechanism remains unknown. We investigated the morphological and physiological characteristics as well as iTRAQ-based proteomics analyses in this study under different combinations [CO] (400 and (800 ± 20) μmol·mol) and water conditions (no, moderate and severe drought stress simulated by polyethylene glycol 6000).

Comparison of a newly developed binary typing with ribotyping and multilocus sequence typing methods for Clostridium difficile.

Clostridium difficile is the causative pathogen for antibiotic-related nosocomial diarrhea. For epidemiological study and identification of virulent clones, a new binary typing method was developed for C. difficile in this study.

[Expression, purification and characterization of recombinant onconase expressed in Pichia pastoris].

Ranpirnase (onconase, ONC) is a new drug, with weak RNase activity and strong cytotoxicity to various tumor cells in vitro and in vivo. This study is to obtain recombination onconase (rONC) with high bioactivity. Based on the codon preference of Pichia pastoris, we designed and synthesized the gene according to cDNA sequences of ONC and the α mating factor's prepeptide.

[Influence of anti-ICOS antibody on quantity and function of CD4(+);CD25(+);Foxp3(+);Treg from lymph and blood of rats with bronchial asthma].

Aim: To investigate the influences of anti-ICOS antibody (anti-ICOSAb) on quantity and function of CD4(+);CD25(+);Foxp3(+);Treg cells from lymph and peripheral blood of rats with bronchial asthma.

Methods: The mononuclear cells (MNC) from lymph and blood were co-cultured with anti-ICOSAb, and then the percentage of CD4(+);CD25(+);Foxp3(+);Treg cells were analyzed by flow cytometer (FCM) and the levels of IL-10 and TGF-β1 in supernatants were determined by ELISA.

Results: The MNC were collected from lymph and blood at 0, 24 and 48 h after the last challenge, respectively, and the cells were cultured for 96 h in vitro.

[Changes and the clinical significance of CD4⁺ CD25⁺ regulatory T cells and Th17 cells in peripheral blood of infants with respiratory syncytial virus bronchiolitis].

Aim: To observe the percentages of CD4(+);CD25(+); regulatory T cells (Tregs) and Th17 cells and the levels of IL-10, TGF-β and IL-17 in peripheral blood of infants with respiratory syncytial virus (RSV) bronchiolitis. The relationship between above cells, cytokines and RSV bronchiolitis was determined.

Methods: Thirty-three infants with RSV bronchiolitis, twenty-eight infants with non-RSV pneumonia and twenty-six healthy infants were enrolled.

[Expression of RhoA in the lung tissue of acute lung injury rats and the influence of RhoA on the expression of IL-8 and IL-10].

Aim: To investigate the expression of RhoA in the lung tissue of acute lung injury (ALI) rats induced by oleic acid (OA) and explore the influence of RhoA on the level of IL-8 and IL-10 in serum. Discussion RhoA and acute lung injury relationship.

Methods: Establish rat oleic acid acute lung injury model, Wet/Dry weights (W/D) were detected.