A solitary osteolytic lesion with pathological fracture in the cervical spine - a case report.

Background: Langerhans cell histiocytosis (LCH) is a rare disorder. The treatment options vary depending on how many organs are involved and how extensive the disease is. In this report, a case of LCH with isolated 6th cervical vertebra (C6) collapse was presented.

Simultaneous determination of fat-soluble vitamins and carotenoids in human serum using a nanostructured ionic liquid based microextraction method.

The determination of fat-soluble vitamins and carotenoids in human serum provides reliable information for diagnosing malnutrition and for establishing appropriate intervention programs. Due to the complex composition of the biological samples, the efficient sample preparation is the key to the analysis. We report here a surface active ionic liquid (SAIL)-based dispersive liquid-liquid microextraction (DLLME) method coupled with a high performance liquid chromatography (HPLC) to determine four fat-soluble vitamins and six carotenoids in human serum simultaneously.

A paper-based ELISA for rapid sensitive determination of anaphylaxis-related MRGPRX2 in human peripheral blood.

Mas-related G-protein-coupled receptor X2 (MRGPRX2) has recently been reported to be associated with anaphylaxis. Detection of MRGPRX2 levels in human peripheral blood might serve as a powerful tool for predicting the predisposition of patients to anaphylactic reactions. For rapid measurement of MRGPRX2, we established a paper-based double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) using mouse monoclonal antibody and horseradish peroxidase (HRP)-labelled rabbit polyclonal antibody as capture antibody and detection antibody, respectively.

Prognostic factors and its predictive value in patients with metastatic spinal cancer.

Background: The spine is the most common location of metastatic diseases. Treating a metastatic spinal tumor depends on many factors, including patients' overall health and life expectancy. The present study was conducted to investigate prognostic factors and clinical outcomes in patients with vertebral metastases.