Identification of five types of forensic body fluids based on stepwise discriminant analysis.

Peripheral blood, menstrual blood, semen, saliva and vaginal secretions are the five most common body fluids found at crime scenes, and the identification of these five body fluids is of great significance to the reconstruction of a crime scene and resolution of the case. However, accurate identification of these five body fluids is still a challenge. To address this problem, a mathematical model for differentiating five types of forensic body fluids based on the differential expression characteristics of multiple miRNAs in five body fluids (peripheral blood, menstrual blood, semen, saliva and vaginal secretions) was developed.

A stepwise strategy to distinguish menstrual blood from peripheral blood by Fisher's discriminant function.

Blood samples are the most common and important biological samples found at crime scenes, and distinguishing peripheral blood and menstrual blood samples is crucial for solving criminal cases. MicroRNAs (miRNAs) are important molecules with strong tissue specificity that can be used in forensic fields to identify the tissue properties of body fluid samples. In this study, the relative expression levels of four different miRNAs (miR-451, miR-205, miR-214 and miR-203) were analysed by real-time PCR, with 200 samples from 5 different body fluids, including two kinds of blood samples (peripheral blood and menstrual blood) and three kinds of non-blood samples (saliva, semen and vaginal secretion).

Long-term postoperative outcomes of bilateral lateral rectus recession vs unilateral recession-resection for intermittent exotropia.

Aim: To discuss the long-term postoperative results of bilateral lateral rectus recession (BLR) and unilateral lateral rectus recession-medial rectus resection (RR) in therapy of intermittent exotropia.

Methods: We retrospectively analyzed 213 cases of intermittent exotropia who underwent surgery between 2008 and 2010. The patients were grouped into BLR group and RR group.

KIF21A mutations in two Chinese families with congenital fibrosis of the extraocular muscles (CFEOM).

Purpose: Two Chinese families (XT and YT) with congenital fibrosis of the extraocular muscles (CFEOM) were identified. The purpose of this study was to determine if previously described Homo sapiens kinesin family member 21A (KIF21A) mutations were responsible for CFEOM in these two Chinese pedigrees.

Methods: Clinical characterization and genetic studies were performed.

Therapeutic effects of two anastomoses of lacrimal passage on canalicular laceration.

Objective: To evaluate the therapeutic effects of two anastomoses (canaliculus-to-lacrimal sac anastomosis and end-to-end anastomosis) on nasolacrimal laceration for over 7 mm from the broken end to the dacryon.

Methods: A total of 71 patients (44 males and 27 females, aged 16-55 years, mean equal to 34.32 years) with fresh canalicular laceration were treated in our hospital from March 2003 to April 2008.

[The changes of extracellular matrix molecules in the resected medial rectus muscle of patients with concomitant exotropia].

Objective: In order to have a better understanding about the role of the extracellular matrix molecules in the intermittent and constant exotropia, we measured the amounts of fibronectin and proteoglycan in the resected medial rectus muscles of patients with concomitant exotropia.

Methods: Thirty-one exotropic patients (including intermittent exotropia 17 cases and constant exotropia 14 cases) and 21 normal were chosen. In exotropic group, there were 7 cases with positive family history.

[Clinical investigation of the syndrome of congenital fibrosis of extraocular muscles].

Objective: To analyze the clinical manifestations of congenital fibrosis of the extraocular muscles (CFEOM) both in their pedigrees and sporadic cases.

Methods: Six families and seven sporadic cases of CFEOM were retrospectively analyzed in the study. The clinical features including genetic characteristics, sex, age of first visit, major complains, subclassifications, features of ptosis and aberrant innervation were evaluated.