The Discovery of Oxathiapiprolin as a Potential Agent for the Control of Litchi Downy Blight Caused by and the Study of Its Mechanism of Action.

Oxathiapiprolin, a novel fungicide targeting oxysterol-binding proteins (OSBP), has proven to be effective against various oomycete diseases, but its effects on pathogens remain underexplored. In this study, we demonstrated that oxathiapiprolin inhibits mycelium growth, sporangium production, zoospore release, and oospore production in , with 50% effective concentrations ranging from 0.000153 to 0.

PDGFR-α Mediated the Neuroinflammation and Autophagy via the JAK2/STAT3 Signaling Pathway Contributing to Depression-Like Behaviors in Myofascial Pain Syndrome Rats.

Depression often occurs in patients with additional co-morbidities, particularly in cases of chronic pain. Currently, there is a lack of research on the molecular mechanisms of depression under chronic pain conditions and suitable animal models. Due to the contradiction exhibited by platelet-derived growth factor receptor (PDGF/PDGFR) in neuroprotection, further investigation is required.

Platelet-derived Growth Factor Receptor-α Induces Contraction Knots and Inflammatory Pain-like Behavior in a Rat Model of Myofascial Trigger Points.

Background: Myofascial trigger points (MTrPs) are the primary etiological characteristics of chronic myofascial pain syndrome. Receptor tyrosine kinases (RTKs) are associated with signal transduction in the central mechanisms of chronic pain, but the role of RTKs in the peripheral mechanisms of MTrPs remains unclear. The current study aimed to identify RTKs expression in MTrPs and elucidate the molecular mechanisms through which platelet-derived growth factor receptor-α (PDGFR-α) induces contraction knots and inflammatory pain-like behavior in a rat model of myofascial trigger points.

Cas-OPRAD: a one-pot RPA/PCR CRISPR/Cas12 assay for on-site root rot detection.

is a devastating plant pathogen that causes soybean root rot worldwide. Early on-site and accurate detection of the causal pathogen is critical for successful management. In this study, we have developed a novel and specific one-pot RPA/PCR-CRISPR/Cas12 assay for on-site detection (Cas-OPRAD) of root rot ().

Comparative Evaluation of PCR-Based, LAMP and RPA-CRISPR/Cas12a Assays for the Rapid Detection of .

Southern stem canker (SSC) of soybean, attributable to the fungal pathogen , results in considerable losses of soybean in the field and has damaged production in several of the main soybean-producing countries worldwide. Early and precise identification of the causal pathogen is imperative for effective disease management. In this study, we performed an RPA-CRISPR/Cas12a, as well as LAMP, PCR and real-time PCR assays to verify and compare their sensitivity, specificity and simplicity and the practicality of the reactions.

The Emerging Role of as Candidate Targets for Engineering Crops with Broad-Spectrum Disease Resistance.

Plant diseases caused by pathogens result in a marked decrease in crop yield and quality annually, greatly threatening food production and security worldwide. The creation and cultivation of disease-resistant cultivars is one of the most effective strategies to control plant diseases. Broad-spectrum resistance (BSR) is highly preferred by breeders because it confers plant resistance to diverse pathogen species or to multiple races or strains of one species.

Rapid Visual Detection of on Lychees Using Recombinase Polymerase Amplification Combined with Lateral Flow Assay Based on the Unique Target Gene .

Downy blight, caused by , is a destructive disease that impacts lychee fruit throughout the pre-harvest, post-harvest, and transportation phases. Therefore, the prompt and precise identification of is crucial for the effective management of the disease. A novel gene encoding a Rh-type ammonium transporter, , was identified in through bioinformatic analysis in this study.

Autophagy-related protein PlATG2 regulates the vegetative growth, sporangial cleavage, autophagosome formation, and pathogenicity of .

Autophagy is an intracellular degradation process that is important for the development and pathogenicity of phytopathogenic fungi and for the defence response of plants. However, the molecular mechanisms underlying autophagy in the pathogenicity of the plant pathogenic oomycete , the causal agent of litchi downy blight, have not been well characterized. In this study, the autophagy-related protein ATG2 homolog, PlATG2, was identified and characterized using a CRISPR/Cas9-mediated gene replacement strategy in .

PlAtg8-mediated autophagy regulates vegetative growth, sporangial cleavage, and pathogenesis in .

is the pathogen of litchi downy blight, which is the most serious disease in litchi. Autophagy is an evolutionarily conserved catabolic process in eukaryotes. Atg8 is a core protein of the autophagic pathway, which modulates growth and pathogenicity in the oomycete .

The complete mitochondrial genome of (Gray, 1846) (Lepidoptera: Nymphalidae) and phylogenetic analysis.

In this study, the complete mitochondrial genome (mitogenome) of (Gray, 1846) (Lepidoptera: Nymphalidae) was first reported with 15,298 bp in size, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes ( and ), and one control region. The nucleotide composition of the entire mitogenome is highly A + T biased (81.5%).

S100A1 expression is increased in spinal cord injury and promotes inflammation, oxidative stress and apoptosis of PC12 cells induced by LPS via ERK signaling.

Spinal cord injury (SCI) is a severe neurological disorder and the molecular mechanisms leading to its poor prognosis remain to be elucidated. S100A1, a mediator of Ca2+ handling of sarcoplasmic reticulum and mitochondrial function, operates as an endogenous danger signal (alarmin) associated with inflammatory response and tissue injury. The aim of the present study was to investigate the expression and biological effects of S100A1 in SCI.

Activity and Point Mutation G699V in PcoORP1 Confer Resistance to Oxathiapiprolin in Field Isolates.

The oxysterol-binding protein inhibitor oxathiapiprolin is a new fungicide for controlling oomycetes diseases. Besides, laboratory mutagenesis oxathiapiprolin-resistance among phytopathogenic oomycetes in the field remains unknown. Here, the sensitivity of 97 isolates to oxathiapiprolin was examined that were collected between 2011 and 2016.

Use of oxathiapiprolin for controlling soybean root rot caused by Phytophthora sojae: efficacy and mechanism of action.

Background: Oxathiapiprolin is a new isoxazoline fungicide developed by DuPont to control oomycete diseases. Although oxathiapiprolin has shown strong inhibitory activity against oomycete pathogens, little is known about its ability to control Phytophthora sojae.

Results: Oxathiapiprolin showed high inhibitory activity against Phytophthora sojae, with 50% effective concentration (EC ) values ranging from 1.

Exploring the trend in religious diversity: Based on the geographical perspective.

The formation and development of religious diversity is a manifestation of the free expression of human thought, belief, and practice, as well as a historical premise and ideological condition for the gradual recognition and integration of modern religions into modern political values. This study examines the spatial characteristics of the development of the global religious diversity index (RDI) and the evolution trend through a geographical perspective by the LISA space-time transition and convergence test. The results show that: (1) At the temporal level, RDI showed a fast and then slow increase after WWII, with an increase of 61.

Ibuprofen on Proliferation and Apoptosis of Sarcoma Cells via PI3K/Akt/mTOR Signaling Pathway.

Nowadays, there is a serious lack of information about the value-added apoptosis of sarcoma cells in China. Especially in clinical medicine, exploring the effect of ibuprofen on the growth and apoptosis of fibrosarcoma cells under the PI3K/Akt/mTOR signaling pathway can not only effectively prevent us in advance, but also be a great way to break through this field. The main purpose of this study was to investigate the effects of ibuprofen on the proliferation, cell cycle and apoptosis of fibrosarcoma cells through the PI3K/Akt/mTOR signaling pathway.

Identification and First Report of Leaf Blight Caused by Phytophthora palmivora on Chinese Water Bamboo in China.

Chinese water bamboo (Dracaena sanderiana) is a popular houseplant due to its ability to survive in various indoor conditions. In October 2020, pronounced leaf blight symptoms with approximately 50% disease incidence were observed on water bamboo in the 35-ha field of Wenchang county (19°50'45'' N, 110°21'38'' E), Hainan, China. The diseased leaves showed pale green with yellowish lesions, dried and shrivelled tips.

A Putative P-Type ATPase Regulates the Secretion of Hydrolytic Enzymes, Phospholipid Transport, Morphogenesis, and Pathogenesis in .

is an important plant pathogenic oomycete with multiple hosts. The P4-ATPases, aminophospholipid translocases (APTs), play essential roles in the growth and pathogenesis of fungal pathogens. However, the function of P4-ATPase in remains unclear.

Translation Initiation Factor eIF4E Positively Modulates Conidiogenesis, Appressorium Formation, Host Invasion and Stress Homeostasis in the Filamentous Fungi .

Translation initiation factor eIF4E generally mediates the recognition of the 5'cap structure of mRNA during the recruitment of the ribosomes to capped mRNA. Although the eIF4E has been shown to regulate stress response in positively, there is no direct experimental evidence for the contributions of eIF4E to both physiological and pathogenic development of filamentous fungi. We generated () gene deletion strains using homologous recombination strategies.

Genome Sequence Resource of from China Using Nanopore Sequencing Technology.

is a destructive oomycete pathogen of taro (), which causes taro leaf blight. To date, only one highly fragmented Illumina short-read-based genome assembly is available for this species. To address this problem, we sequenced strain Lyd2019 from China using Oxford Nanopore Technologies long-read sequencing and Illumina short-read sequencing.

Genome Sequence Resource of the Causal Agent of Stem and Root Rot of Cowpea.

The causal agent of stem and root rot of cowpea, , is a widely distributed species of the genus. Here, we generate a high-quality complete genome assembly of PSY2020 (89.39 Mb, N 2.

Phosphite translocation in soybean and mechanisms of Phytophthora sojae inhibition.

Although phosphite (Phi)-based fertilizers are used in large quantities in agriculture, the use of Phi-based fungicides against soybean root rot caused by Phytophthora sojae are limited. While, their low toxicity are of high ecological and economic focus. Limited attention has been paid to Phi translocation efficiency in soybeans and the efficacy of Phi as a fungicide against P.

Genome Sequence Data of , an Oomycete Pathogen Causing Litchi Downy Blight.

is an oomycete pathogen that exclusively infects litchi, with infection stages affecting a broad range of tissues. In this study, we obtained a near chromosome-level genome assembly of ZL2018 from China using Oxford Nanopore Technologies long-read sequencing and Illumina short-read sequencing. The genome assembly was 64.

Antifungal activity of liquiritin in Phytophthora capsici comprises not only membrane-damage-mediated autophagy, apoptosis, and Ca reduction but also an induced defense responses in pepper.

Phytophthora capsici causes a severe soil-borne disease in a wide variety of vegetables; to date, no effective strategies to control P. capsici have been developed. Liquiritin (LQ) is a natural flavonoid found in licorice (Glycyrrhiza spp.