Genetically modified indigenous Pseudomonas aeruginosa drove bacterial community to change positively toward microbial enhanced oil recovery applications.

Aims: Microbial enhanced oil recovery (MEOR) is cost-effective and eco-friendly for oil exploitation. Genetically modified biosurfactants-producing high-yield strains are promising for ex-situ MEOR. However, can they survive and produce biosurfactants in petroleum reservoirs for in-situ MEOR? What is their effect on the native bacterial community?

Methods And Results: A genetically modified indigenous biosurfactants-producing strain Pseudomonas aeruginosa PrhlAB was bioaugmented in simulated reservoir environments.

High mono-rhamnolipids production by a novel isolate Pseudomonas aeruginosa LP20 from oily sludge: characterization, optimization, and potential application.

This study aims to isolate microbial strains for producing mono-rhamnolipids with high proportion. Oily sludge is rich in petroleum and contains diverse biosurfactant-producing strains. A biosurfactant-producing strain LP20 was isolated from oily sludge, identified as Pseudomonas aeruginosa based on phylogenetic analysis of 16S rRNA.

Improving Rhamnolipids Biosynthesis in sp. L01 through Atmospheric and Room-Temperature Plasma (ARTP) Mutagenesis.

Biosurfactants have significant applications in various industries, including microbial-enhanced oil recovery (MEOR). While the state-of-the-art genetic approaches can generate high-yield strains for biosurfactant production in fermenters, there remains a critical challenge in enhancing biosurfactant-producing strains for use in natural environments with minimal ecological risks. The objectives of this work are enhancing the strain's capacity for rhamnolipids production and exploring the genetic mechanisms for its improvement.

Intradural Extramedullary Nerve Sheath Myxoma of the Cervical Spine: A Case Report and Review of Literature.

Nerve sheath myxoma is a rare benign soft tissue tumor. Intraspinal nerve sheath myxomas are rare. Only 8 cases of intraspinal nerve sheath myxoma have been reported to date, and no case of nerve sheath myxoma has been reported in the cervical spinal canal.

Glycerol or crude glycerol as substrates make Pseudomonas aeruginosa achieve anaerobic production of rhamnolipids.

Background: The anaerobic production of rhamnolipids is significant in research and application, such as foamless fermentation and in situ production of rhamnolipids in the anoxic environments. Although a few studies reported that some rare Pseudomonas aeruginosa strains can produce rhamnolipids anaerobically, the decisive factors for anaerobic production of rhamnolipids were unknown.

Results: Two possible hypotheses on the decisive factors for anaerobic production of rhamnolipids by P.

Athermalization of dual-waveband infrared systems containing diffractive optical elements via optical-digital joint design.

Because of material limitations, achieving an athermal design for dual-waveband infrared systems is difficult. This study integrates single-layer diffractive elements to reduce the volume and weight of such a design and introduces optical-digital joint methods to eliminate the impact of low diffraction efficiency. To achieve athermalization, temperature polychromatic integral diffraction efficiency and temperature integral wavelength weight are incorporated in the point spread function (PSF) model.

Human adipose-derived mesenchymal stem cells inhibit proliferation and induce apoptosis of human gastric cancer HGC-27 cells.

The aim of this study was to explore the effects of human adipose-derived mesenchymal stem cells (ASCs) on the growth of gastric cancer cells in vivo and vitro and its mechanism. ASCs were isolated from abandoned adipose tissues, and the surface markers were identified by flow cytometry. In vitro experiments, HGC-27 cells cultured in ASCs-conditioned medium (CM) were assigned as the experimental group, while HGC-27 cells cultured in normal medium were as the control group.

[Effect of NO produced by indigenous denitrifiers in oil reservoir on the physical properties of crude oil].

The success of microbial enhanced oil recovery (MEOR) relies on complex microbial processes. Nevertheless, the contribution and mechanism of in-situ denitrification to microbial oil recovery remain unclear. In this study, eight denitrifying bacterial strains, designated T1, D1, D44, D46, D15, S1, S2 and S6, were isolated from the produced water of Xinjiang Oilfield, China, by a double layered plate method.

Achromatic annular folded lens with reflective-diffractive optics.

This paper proposes an achromatic annular folded lens (AFL) with a reflective-diffractive optical element (RDOE). We derive novel mathematical models of the diffraction efficiency and polychromatic integral diffraction efficiency (PIDE) of the RDOE and an expression for its microstructure height. An AFL with an RDOE made of an optical plastic substrate material is designed in the visible waveband.

Design of dual-band infrared zoom lens with multilayer diffractive optical elements.

A mid-wave infrared (MWIR)/long-wave infrared (LWIR) dual-band zoom lens design with multilayer diffractive optical elements (MLDOEs) is presented. The mathematical relationship between the substrate material selection for dual-band MLDOE and polychromatic integral diffraction efficiency (PIDE) is deduced in the oblique incident situation, and further, a method for optimal selection of substrate material is proposed to obtain the high PIDE in an incident angle range. In the optimization process, the optimal substrate material combination is selected based on the proposed method, and the principle of lens material replacement is discussed.

Optical athermalization in the visible waveband using the 1+∑ method.

In this paper, the 1+∑ method, which is based on the double-lens equivalent model, is proposed to achieve optical athermalization in the visible waveband. The glass in the system was replaced by calculating and analyzing the synthetic weights of the thermal power and chromatic power. A complex aerial camera with long focal length was designed over a temperature difference of 100°C.

Effect of the surface roughness on the efficiency of diffractive optical elements.

The proposed study aims to investigate the effect of surface roughness on the efficiency of diffractive optical elements (DOEs) used in imaging optical systems. A mathematical model describing the influence of surface roughness on diffraction efficiency and polychromatic integral diffraction efficiency (PIDE) is presented, and its effects are analyzed and simulated. Examples of single-layer DOEs containing PMMA substrate and multi-layer DOEs composed of a combination of PMMA and polycarbonate substrates for each layer used in the visible region are analyzed.

Design of a built-in baffle for a Ritchey-Chretien optical system.

A baffle effectively prevents direct rays of stray light from entering an optical system. Existing design methods can result in very long outer baffle lengths, increasing the weight and volume of the optical system, or large secondary mirror baffle sizes, increasing the central obscuration and resulting in the reduction of the illumination beam on the image. To overcome these challenges, a design method for a built-in baffle is presented.

PSF model for diffractive optical elements with improved imaging performance in dual-waveband infrared systems.

Single-layer diffractive optical elements (SLDOEs) have advantages in terms of configuration, fabrication, range of angles and cost; however, the diffraction efficiency decreases sharply with wavelength deviating from the design wavelength, especially for dual-waveband imaging, causing apparent image blur. We propose a point spread function (PSF) model affected by the diffraction efficiency, which is called PSF, and a method of restoring the blurred image to improve imaging performance in dual-waveband infrared systems with an SLDOE. Then, a design example of cooled MWIR and LWIR is presented.

Optimization method of multilayer diffractive optical elements with consideration of ambient temperature.

A method for the optimal design of multilayer diffractive optical elements (MLDOEs) with consideration of ambient temperature is presented to improve the image quality over the entire temperature range. The relationship between diffraction efficiency and temperature is analyzed, and an optimization process of surface relief height for the MLDOEs is given. A practical 3-5 μm athermal hybrid optical system with a double-layer diffractive optical element is designed in the temperature range from -20°C to 60°C, and the image quality of two hybrid optical systems with optimized MLDOE and original MLDOE is compared.

Exopolysaccharide production by an indigenous isolate Pseudomonas stutzeri XP1 and its application potential in enhanced oil recovery.

In this study, a rapid and efficient method for screening biopolymer producers was established using 96-well plates. An indigenous biopolymer producer Pseudomonas stutzeri XP1 was isolated from Xinjiang oil reservoirs, China. Strain XP1 can grow and produce 16 g/l biopolymer using corn starch and nitrate.

Optimal design method on diffractive optical elements with antireflection coatings.

The effect of antireflection coatings on diffraction efficiency of diffractive optical elements (DOEs) was studied and the mathematical model of diffraction efficiency affected by antireflection coatings for DOEs is presented. We found antireflection coatings can cause a significant reduction on diffraction efficiency at the designed, or the central wavelength. In order to solve this problem, we proposed a method to keep 100% diffraction efficiency at the designed wavelength by ensuring the 2π phase induced by DOEs and the antireflection coatings.

Substrate material selection method for multilayer diffractive optics in a wide environmental temperature range.

We present a substrate material selection method for multilayer diffractive optical elements (MLDOEs) to obtain high polychromatic integral diffraction efficiency (PIDE) in a wide environmental temperature range. The extended expressions of the surface relief heights for the MLDOEs are deduced with consideration of the influence of the environmental temperature. The PIDE difference Δη¯(λ) and PIDE change factor F are introduced to select a reasonable substrate material combination.

High diffraction efficiency of three-layer diffractive optics designed for wide temperature range and large incident angle.

A mathematical model of diffraction efficiency and polychromatic integral diffraction efficiency affected by environment temperature change and incident angle for three-layer diffractive optics with different dispersion materials is put forward, and its effects are analyzed. Taking optical materials N-FK5 and N-SF1 as the substrates of multilayer diffractive optics, the effect on diffraction efficiency and polychromatic integral diffraction efficiency with intermediate materials POLYCARB is analyzed with environment temperature change as well as incident angle. Therefore, three-layer diffractive optics can be applied in more wide environmental temperature ranges and larger incident angles for refractive-diffractive hybrid optical systems, which can obtain better image quality.

Aberration and boresight error correction for conformal aircraft windows using the inner window surface and tilted fixed correctors.

A static solution to aberrations and boresight error for tilted conformal aircraft windows at different look angles is reported. The solution uses the inner window surface to correct the window aberrations at a 0° look angle and uses fixed correctors behind the window to correct the residual window aberrations at other look angles. Then, the boresight error for the window at different look angles is corrected by tilting the fixed correctors.

Draft genome sequence of Paenibacillus sp. strain A2.

Paenibacillus sp. strain A2 is a Gram-negative rod-shaped bacterium isolated from a mixture of formation water and petroleum in Daqing oilfield, China. This facultative aerobic bacterium was found to have a broad capacity for metabolizing hydrocarbon and organosulfur compounds, which are the main reasons for the interest in sequencing its genome.

Tolerance analysis of multilayer diffractive optics based on polychromatic integral diffraction efficiency.

Multilayer diffractive optical elements (MLDOEs) can achieve high diffraction efficiency for broadband wavelength. Polychromatic integral diffraction efficiency (PIDE) is the key concern for evaluating diffraction efficiency over the waveband. The modulation transfer function of a hybrid refractive-diffractive optical system is directly affected by the PIDE.

Isolation and characterization of a crude oil degrading bacteria from formation water: comparative genomic analysis of environmental Ochrobactrum intermedium isolate versus clinical strains.

In this study, we isolated an environmental clone of Ochrobactrum intermedium, strain 2745-2, from the formation water of Changqing oilfield in Shanxi, China, which can degrade crude oil. Strain 2745-2 is aerobic and rod-shaped with optimum growth at 42 °C and pH 5.5.

Biosurfactant produced by novel Pseudomonas sp. WJ6 with biodegradation of n-alkanes and polycyclic aromatic hydrocarbons.

Alkanes and polycyclic aromatic hydrocarbons (PAHs) have threatened the environment due to toxicity and poor bioavailability. Interest in degradation of these hazardous materials by biosurfactant-producing bacteria has been steadily increasing in recent years. In this work, a novel biosurfactant-producing Pseudomonas sp.

Microbial abundance and community composition influence production performance in a low-temperature petroleum reservoir.

Enhanced oil recovery using indigenous microorganisms has been successfully applied in the petroleum industry, but the role of microorganisms remains poorly understood. Here, we investigated the relationship between microbial population dynamics and oil production performance during a water flooding process coupled with nutrient injection in a low-temperature petroleum reservoir. Samples were collected monthly over a two-year period.