Comparative Study of the Structural Characteristics and Bioactivity of Polysaccharides Extracted from Hemsl. Using Different Solvents.

The polysaccharides extracted from are thought to have anti-urolithiasis activity in Drosophila kidney stones. This study aimed to assess the effects of different extraction solvents on the yield, chemical composition, and bioactivity of polysaccharides from . polysaccharides were extracted by using four solutions: hot water, HCl solution, NaOH solution, and 0.

One novel alkaloid from the stems of .

The 6-methoxy-cannabisin I (), a new alkaloid, together with five known compounds oleraisoindole A (), cannabisin F (), apigenin (), syringin () and ethyl-syringin () were isolated from stems. Their structures were identified by the analysis of spectroscopic data. Compound was isolated from for the first time.

Derivatives of benzamide of the extracts from the bark of Maqian ( var. ).

Two newly discovered natural derivatives of benzamide, -Ethyltembamide () and N-[(Z)-2-(4-Methoxyphenyl)vinyl]benzamide(), together with four known compounds, hortiamide (), N-[(E)-2-(4-Methoxyphenyl)vinyl] benzamide (alatamide) (), dihydroaltamide () and tembamid (), were isolated from the barks of of var. (Huang) Huang. Their structures were determined on the basis of spectral data.

Bioactivity-Guided High Performance Counter-Current Chromatography and Following Semi-Preparative Liquid Chromatography Method for Rapid Isolation of Anti-Inflammatory Lignins from Dai Medicinal Plant, var. .

The development of Dai medicine is relatively slow, and has great economic and medicinal value. It is still difficult to obtain medicinal components from the low-polarity parts of belonging to Dai medicine. In this study, we introduced one simple and quick strategy of separating target compounds from the barks of var.

Polyoxypregnane Glycosides from Root of and Inhibited Nitric Oxide Levels in LPS Stimulated RAW 264.7 Cells.

Six new polyoxypregnane glycosides, marstenacisside F1−F3 (1−3), G1−G2 (4−5) and H1 (6), as well as 3-O-β-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranosyl-11α,12β-di-O-benzoyl-tenacigenin B (7), were isolated from the roots of Marsdenia tenacissima. Their structures were established by an extensive interpretation of their 1D and 2D NMR and HRESIMS data. Compounds 1−7 were tenacigenin B derivatives with an oligosaccharide chain at C-3.

Rhizome Extract Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis: Active Ingredient Investigation and Evaluation.

is an important food spice and ethnic medicine in Southwest China. In this study, we found that the EtOAc-soluble fraction (AHE) of the rhizome ethanol extract could ameliorate dextran sulfate sodium-induced ulcerative colitis (UC). To explore active constituents, five pairs of previously unreported enantiomers (-), together with nine known ones (-), were obtained.

[Preparation of brazilein from by high performance countercurrent chromatography].

Brazilein is among the main chemical constituents of . It has diverse pharmacological activities. Modern pharmacological studies have shown that the compound has antitumor, anti-inflammatory, antibacterial, antioxidant, immunomodulatory, and other pharmacological activities.

Two new monoterpenoid indole alkaloids from stems.

Two previously unreported monoterpenoid indole alkaloids, 19(20)-5-carboxymethylvallesiachotamine () and 19(20)-5-carboxymethylvallesiachotamine (), along with five known indole alkaloids (-) were isolated from stems. Their structures were characterized by extensive spectroscopic data. Among these isolates, compounds and were the two new Δ geometric isomers.

Development of a strategy for a quick process for separation of volatile compounds in counter-current chromatography: purification of cinnamaldehyde from by high performance counter-current chromatography.

It is a challenge for many researchers to separate volatile compounds. In this study, we introduce a rapid and efficient method of separating target compound from the twigs of by high performance counter-current chromatography. Under the bioassay guidance, the total extract exhibited a potential activity against NO production in RAW 264.

Anti-hyperglycaemic effect of labdane diterpenes isolated from the rhizome of Roxb., an edible plant in Southwest China.

Roxb. rhizome is a fork medicine mainly used in South and Southeast Asia. In present study, the hypoglycaemic effects of the ethanolic extract of .

A novel pentacyclic triterpene from the canes of (Rubiaceae).

One novel pentacyclic triterpene, 24-dimethoxymethyl-3,6,19- trihydroxy -12-en-28-oic acid (), along with six known compounds -, were isolated from the canes of Roxb. Their structures were determined according to spectroscopic and spectrometric analysis. The anti-inflammatory activities of the isolated compounds (-) were scanned against NO production in LPS-activated RAW 264.

Theoretical and experimental studies on hydrogen migration in dissociative ionization of the methanol monocation to molecular ions H and HO.

The dissociative ionization processes of the methanol monocation CHOH to H + CHO and HO + CH are studied by method, and hydrogen migration processes are confirmed in these two dissociation processes. Due to the positive charge assignment in dissociation processes, the fragmentation pathways of CHOH to H + CHO and CHOH to HO + CH are also calculated. The calculation results show that a neutral H moiety in the methanol monocation CHOH is the origin of the formation of H , and the ejection of fragment ions H and HO is more difficult than CHO and CH respectively.

Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo.

Countercurrent separation (CCS) has been widely used for the separation of high abundance compounds. However, the identification of low abundance compounds, such as mammalian steroid hormones, from natural sources is still a challenging task. A mixture of 14 human steroid hormone reference compounds was prepared for the development of a CCS enrichment strategy.

Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy.

TLC-based strategies were proposed in 1979 (Hostettmann et al.) and 2005 (Friesen & Pauli; GUESS method) to minimize the number of partitioning experiments required for countercurrent separation (CCS) solvent system selection. As semi-empirical approaches, both proposed that the K values defining the sweet spot of optimal CCS corresponded to a matching Rf value range from the silica gel TLC plate developed in the organic phase of a biphasic or a corresponding monophasic solvent system.

Chemical constituents and anti-inflammatory activities of Maqian (Zanthoxylum myriacanthum var. pubescens) bark extracts.

In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene.

Two new lignans from Saururus chinensis.

Two new lignans, (Z)-14-bis(3',4'-dimethoxyphenyl)-2,3-dimethylbut-2-ene-1,4-dione (1), threo-2-methyl-3-oxo-1-(3',4',5'-trimethoxyphenyl)butyl-3″,4″-dimethoxybenzoate (2), together with 15 known derivatives (3-17) were isolated from Saururus chinensis. Their structures were determined on the basis of spectral data, including 1D and 2D NMR experiments and HREIMS spectra. The antitumour activity was screened by MTT assay, compounds 1, 2, 3, 5, 9-11 and 13-15 showed no cytotoxic activity against HL-60, SMMC-7721, A549, MCF-7 and SW480 cell lines.

Quick method for separating target compounds from the bark of Maqian (Zanthoxylum myriacanthum var. pubescens) by high-performance countercurrent chromatography.

Choosing a suitable solvent system for a countercurrent chromatography separation presents a challenge for many researchers. In this study, we introduce a quick method of separating a target compound from the bark of Zanthoxylum myriacanthum var. pubescens by countercurrent chromatography.

A new isoflavone glycoside from Pueraria alopecuroides.

A new isoflavone glycoside, (-)-tuberosin-3-O-β-D-glucopyranoside (1), along with 10 known compounds 1a-10, was isolated from Pueraria alopecuroides. Their structures were determined on the basis of spectral data including 1D and 2D NMR and HREIMS. These compounds were isolated from this plant for the first time.

One new flavonoid from Oroxylum indicum.

One new flavonoid, 5,6,7-trimethoxyflavone-8-O-β-D-glucopyranoside (1), along with six known compounds 2-7, was isolated from Oroxylum indicum. Their structures were determined on the basis of spectral data. The antibacterial activities of compounds 1-4 were studied.