A GMP-compliant manufacturing method for Wharton's jelly-derived mesenchymal stromal cells.

Background: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) hold great therapeutic potential in regenerative medicine. Therefore, it is crucial to establish a Good Manufacturing Practice (GMP)-compliant methodology for the isolation and culture of WJ-MSCs. Through comprehensive research, encompassing laboratory-scale experiments to pilot-scale studies, we aimed to develop standardized protocols ensuring the high yield and quality of WJ-MSCs manufacturing.

Antipathogenic Activities of Volatile Organic Compounds Produced by LT1 against LC1, the Pathogen of Southern Blight in .

This study explores the antipathogenic properties of volatile organic compounds (VOCs) produced by LT1, isolated from the rhizosphere soil of . The impact of these VOCs on the mycelial growth of LC1, the causative agent of southern blight in , was evaluated using a double Petri-dish assay. The biocontrol efficacy of these VOCs was further assessed through leaf inoculation and pot experiments.

The Impact of COVID-19 on Small Businesses in the US: A Longitudinal Study from a Regional Perspective.

Small businesses have suffered disproportionately from the COVID-19 pandemic. We use near-real-time weekly data from the Small Business Pulse Survey (April 26, 2020 - June 17, 2021) to examine the constantly changing impact of COVID-19 on small businesses across the United States. A set of multilevel models for change are adopted to model the trajectories of the various kinds of impact as perceived by business owners (subjective) and those recorded for business operations (objective), providing insights into regional resilience from a small business perspective.

LT1: a potential biocontrol agent for southern blight on .

Introduction: Southern blight, caused by , poses a serious threat to the cultivation of , a plant with significant medicinal value. The overreliance on fungicides for controlling this pathogen has led to environmental concerns and resistance issues. There is an urgent need for alternative, sustainable disease management strategies.

Characteristics of ZjCIPKs and ZjbHLH74-ZjCIPK5 regulated cold tolerance in jujube.

CIPKs are kind of serine/threonine (Ser/Thr) protein kinases which play important roles in response to biotic and abiotic stresses, and in plant growth and development. However, CIPKs in jujube (Ziziphus jujuba Mill.) had limited information, especially regarding their response to cold stress.

De Novo RNA Sequencing and Transcriptome Analysis of Gene Expression during Sclerotium Development.

is a destructive soil-borne fungal pathogen that causes stem rot in cultivated plants. However, little is known about the genetic basis of sclerotium development. In this study, we conducted de novo sequencing of genes from three different stages of (mycelia, early sclerotium formation, and late sclerotium formation) using Illumina HiSeq 4000.

Asymmetric α-Allylation of Glycinate with Switched Chemoselectivity Enabled by Customized Bifunctional Pyridoxal Catalysts.

Owing to the strong nucleophilicity of the NH group, free-NH glycinates react with MBH acetates to usually deliver N-allylated products even in the absence of catalysts. Without protection of the NH group, chiral pyridoxal catalysts bearing an amide side chain at the C3 position of the naphthyl ring switched the chemoselectivity of the glycinates from intrinsic N-allylation to α-C allylation. The reaction formed chiral multisubstituted glutamic acid esters as S 2'-S 2' products in good yields with excellent stereoselectivity (up to 86 % yield, >20 : 1 dr, 97 % ee).

LncRNA PITPNA-AS1 as a Potential Diagnostic Marker and Therapeutic Target Promotes Hepatocellular Carcinoma Progression via Modulating miR-448/ROCK1 Axis.

Long non-coding RNAs are critical to hepatocellular carcinoma (HCC) developments. LncRNA PITPNA antisense RNA 1 (PITPNA-AS1) is a new regulator in several tumors. However, the mechanism by which PITPNA-AS1 mediates the tumorigenesis of HCC remains unclear.

Emodin Enhances the Chemosensitivity of Endometrial Cancer by Inhibiting ROS-Mediated Cisplatin-resistance.

Background: Endometrial cancer is a common cause of death in gynecological malignancies. Cisplatin is a clinically chemotherapeutic agent. However, drug-resistance is the primary cause of treatment failure.

Degradation of lignin in birch sawdust treated by a novel Myrothecium verrucaria coupled with ultrasound assistance.

Combined treatment of a novel fungal endophyte Myrothecium verrucaria coupled with ultrasound assistance was conducted to enhance lignin degradation in birch sawdust. The optimum treatment conditions were confirmed as the materials to liquid ratio 1:20, temperature 30°C, time 4days and pH 7, respectively. The results showed that the combined treatment led to the lignin degradation reaching 67.

Production of Laccase by a New Myrothecium verrucaria MD-R-16 Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.] and its Application on Dye Decolorization.

The present study was conducted to screen a laccase-producing fungal endophyte, optimize fermentation conditions, and evaluate the decolorization ability of the laccase. A new fungal endophyte capable of laccase-producing was firstly isolated from pigeon pea and identified as based on a ITS-rRNA sequences analysis. Meanwhile, various fermentation parameters on the laccase production were optimized via response surface methodology (RSM).

The level of socioeconomic development of EU countries and the state of ISO 14001 certification.

This study examines the relationship between the level of socioeconomic development of the EU 28 countries and the adoption of International Organization for Standardization (ISO) 14001 environmental management system. First, through a multivariate comparative analysis of the secondary data obtained from the public statistics, a Hellwig's synthetic indicator is created to rank the level of socioeconomic development of the EU 28 countries. Then, using the total number of certificates issued in 2012 and the increase from 2011, this study has found a correlation between the level of national socioeconomic development and the adoption of ISO 14001 system in their businesses.

Association of coronary artery calcium with bone mineral density in postmenopausal women.

Objectives: Atherosclerosis and osteoporosis (OP) are common diseases in elderly individuals and may share common pathogenetic mechanisms. The aim of this study was to investigate the association between bone mineral density (BMD) and coronary artery calcium (CAC) in postmenopausal women.

Methods: In this cross-sectional study, 186 postmenopausal women 50-80 years of age were included.

Segregated and synchronized vector solutions to linearly coupled systems of Schrödinger equations.

In this paper, we study the following linearly coupled system [Formula: see text], where ε > 0 is a small parameter, () are positive potentials, and = > 0 ( ≠ ) are coupling constants for , = 1, …, . We investigate the effect of potentials to the structure of the solutions. More precisely, we construct multi-spikes solutions concentrating near the local maximum point [Formula: see text] of ().

Performance Evaluation of the Three-Layer ONIOM Method: Case Study for a Zwitterionic Peptide.

The performance of the three-layer ONIOM method was systematically investigated by comparing the optimized geometries and calculated deprotonation energy of a zwitterionic peptide molecule, NH3(+)-CH(n)Bu-CO-NH-CH2-CO-NH-CH(n)Bu-COO(-), using all possible combinations of B3LYP/6-31G* as the high-level quantum (HQ), AM1 as the low-level quantum (LQ), and Amber as the MM method. Results show that the three-layer ONIOM(HQ:LQ:MM) method, which includes a medium-level quantum method in the middle system to take into account the electronic effects of the middle layer and to keep the problematic QM-MM boundary away from the action region, is more reliable and more stable than the QM:MM:MM or generic QM/MM method and is the best compromise between accuracy and computational cost.

Does dinitrogen hydrogenation follow different mechanisms for [(eta5-C5Me4H)2Zr]2(mu2,eta2,eta2-N2) and {[PhP(CH2SiMe2NSiMe2CH2)PPh]Zr}2(mu2,eta2,eta2-N2) complexes? A computational study.

The mechanisms of dinitrogen hydrogenation by two different complexes--[(eta(5)-C(5)Me(4)H)(2)Zr](2)(mu(2),eta(2),eta(2)-N(2)), synthesized by Chirik and co-workers [Nature 2004, 427, 527], and {[P(2)N(2)]Zr}(2)(mu(2),eta(2),eta(2)-N(2)), where P(2)N(2) = PhP(CH(2)SiMe(2)NSiMe(2)CH(2))(2)PPh, synthesized by Fryzuk and co-workers [Science 1997, 275, 1445]--are compared with density functional theory calculations. The former complex is experimentally known to be capable of adding more than one H(2) molecule to the side-on coordinated N(2) molecule, while the latter does not add more than one H(2). We have shown that the observed difference in the reactivity of these dizirconium complexes is caused by the fact that the former ligand environment is more rigid than the latter.