[Strategies to choose scaffold materials for tissue engineering].

Current therapies of organ failure or a wide range of tissue defect are often not ideal. Transplantation is the only effective way for long time survival. But it is hard to meet huge patients demands because of donor shortage, immune rejection and other problems.

[Optimization of the method for isolating and culturing rat mesenchymal stem cells].

Objective: To optimize the protocols for isolation and culture of mesenchymal stem cells from rat bone marrow (BMSCs).

Methods: BMSCs were isolated by adherence to plastic with frequent medium change and reduced trypsinization time. The cell growth curves were drawn and the surface markers of BMSCs were detected by flow cytometry.

An in situ electrochemical detection method of cell viability.

An in situ electrochemical method of cell viability, which integrated cell culture, pretreatment and detection in a cell culture dish, was developed. The method significantly improved the electrochemical response of cells, simplified the operation process, reduced the experiment time, avoided the use of trypsin, and was applied in the study of the effectiveness of antitumor drugs on tumor suppression.