Biothiols-activated near-infrared frequency up-conversion luminescence probe for early evaluation of drug-induced hepatotoxicity.

A novel biothiols-sensitive near-infrared (NIR) fluorescent probe RhDN based on a rhodamine skeleton was developed for early detection of drug-induced hepatotoxicity in living mice. RhDN can be used not only as a conventional large stokes shift fluorescent (FL) probe, but also as a kind of anti-Stokes frequency upconversion luminescence (FUCL) molecular probe, which represents a long wavelength excitation (808 nm) to short wavelength emission (760 nm), and response to Cys/Hcy/GSH with high sensitivity. Compared with traditional FL methods, the FUCL method exhibited a lower detection limit of Cys, Hcy, and GSH in 75.

A facile near-infrared xanthene fluorescence probe for visualizing of hypochlorous acid in vitro and in vivo.

Background: Hypochlorous acid (HClO) is an important biomarker for inflammation, cardiovascular disease, and even cancer. It is of great significance to accurately monitor and quantitatively analyze the fluctuations of HClO to better understand their physiological functions. Traditional HClO detection methods such as high-performance liquid chromatography (HPLC), and mass spectrometry are preferred, but are costly and unsuitable in vivo.

Polymethine Molecular Platform for Ratiometric Fluorescent Probes in the Second near-Infrared Window.

Visualizing biomolecules such as enzymes in the deep tissue of living organisms via molecular ratiometric fluorescent probes in the second near-infrared window (NIR-II) with a built-in self-calibration function can provide reliable information about relevant pathophysiological processes directly but so far is not feasible due to the lack of a fluorescence modulation strategy in the NIR-II window. Here we present a molecular platform Py-2 by integrating the rhodamine 6G scaffold and polymethine. The maximal emission wavelength of Py-2 was 1010 nm and blue-shifted to 945 nm when its secondary amine was acylated.

Morphologically Flex Sm-MOF Based Electrochemical Immunosensor for Ultrasensitive Detection of a Colon Cancer Biomarker.

Despite having the potential to synthesize stable metal-organic frameworks (MOFs), rare earth metal-based MOFs have not been exploited extensively. Owing to the high coordination numbers, the MOFs can generate a suitable coordination environment for various applications. Herein, samarium (Sm)-based MOFs were synthesized with three different organic linkers, namely, trimesic acid (TMA), -tetra(4-carboxyphenyl)porphine (TCPP), and 1,3,6,8-tetra(4-carboxylphenyl) pyrene(TBPy) by the solvothermal approach.

Label-Free Electrochemical Immunosensor for Ultrasensitive Detection of Carbohydrate Antigen 125 Based on Antibody-Immobilized Biocompatible MOF-808/CNT.

In this work, a nanocomposite of Zr-trimesic acid MOF (MOF-808) with carbon nanotube (CNT) was synthesized through an in situ formation of MOF-808 on the activated CNT. The synthesized materials were characterized by powder X-ray diffraction, transmission electron microscopy, X-ray photoluminescence spectroscopy, Brunauer-Emmett-Teller, Fourier transform infrared spectroscopy, and Raman spectroscopy. The protein compatible nature with high surface area and electrocatalytic ability of MOF-808 was utilized to construct an immunosensor for ultra low-level detection of the ovarian cancer biomarker, carbohydrate antigen 125 (CA 125).

Magnetic graphene oxide-ultrathin nickel-organic framework composite for the extraction and determination of epoxiconazole in food samples.

In this work, a magnetic graphene oxide-ultrathin metal-organic framework composite (FeO@SiO-GO-Ni-MOF) was synthesized for the first time. Employing FeO@SiO-GO-Ni-MOF composite as extractant, a novel method for the separation and analysis of the pesticide epoxiconazole was established with the assistance of high performance liquid chromatography (HPLC). The adsorption mechanisms were studied including by adsorption kinetics, thermodynamic parameters and adsorption isotherms.

Facile synthesis of novel reduced graphene oxide@polystyrene nanospheres for sensitive label-free electrochemical immunoassay.

Herein, we report the synthesis of novel nanosized reduced graphene oxide@polystyrene nanospheres (rGO@PS NSs) through an electrostatic interaction method, and further their exploitation for highly sensitive label-free electrochemical immunoassay applications. These rGO@PS NSs provide a universal and promising carrier platform to develop excellent biosensors for clinical screening and diagnostic applications.

Three-dimensional porous Cu@CuO aerogels for direct voltammetric sensing of glucose.

Three-dimensional Cu@CuO aerogels with excellent electrocatalytic activity were prepared and used as electrode matrix for constructing novel electrochemical glucose sensors. The aerogels were obtained by adding a fresh solution of NaBH into a mixture of CuCl and NaOH aqueous solutions under stirring at room temperature. The aerogels were assembled with Cu or CuO nanoparticles.

Multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array.

Quantitative detection of multiple chicken cytokines is a good evaluation of cell-mediated immunity in chickens after disease infection or vaccination. However, current assay methods for chicken cytokines cannot meet the needs of clinical diagnosis due to unsatisfactory sensitivity and low assay throughput. Herein, a sensitive chemiluminescence (CL) imaging immunosensor array has been developed for high-throughput detection of multiple chicken cytokines.

A nanozyme tag enabled chemiluminescence imaging immunoassay for multiplexed cytokine monitoring.

We report a new concept of a chemiluminescence imaging nanozyme immunoassay (CINIA), in which nanozymes are exploited as catalytic tags for simultaneous multiplex detection of cytokines. The CINIA provides a novel and universal nanozyme-labeled multiplex immunoassay strategy for high-throughput detection of relevant biomarkers and further disease diagnosis.

Efficient streptavidin-functionalized nitrogen-doped graphene for the development of highly sensitive electrochemical immunosensor.

In this work, an efficient and universal streptavidin-functionalized nitrogen-doped graphene (NG) was for the first time proposed and used to develop a highly sensitive electrochemical immunosensor for the detection of tumor markers. Transmission electron microscopy, electrochemical impedance spectrum, static water contact measurement, and cyclic voltammetry were used to characterize the streptavidin-functionalized NG platform and immunosensor. The biofunctionalized NG showed excellent hydrophilicity, larger specific surface area, and high electrochemical activity.