[Genetic analysis of an individual of Ay serologic phenotype].

Objective: To study the cause of weak expression of Ay antigens on the surface of erythrocytes and understand its molecular characteristics.

Methods: Genotypic analysis of an individual of Ay serologic phenotype was performed using polymerase chain reaction with sequence specific primers (PCR-SSP). Sequence analysis of the 7 exons and some of the introns was carried out with ABI Prism 3100 DNA sequencer, and the Ay gene sequence was compared with A102 reference sequence.

Ex vivo expansion of CD34+ and T and NK cells from umbilical cord blood for leukemic BALB/C nude mouse transplantation.

CBSCT with low incidence of GVHD is associated with higher rates of delayed engraftment and relapse compared with other stem cells transplants. The immune-mediated effect of NK and cytotoxic T cells against residual tumor cells was shown to prevent relapse and reinduce remission after bone marrow transplantation. We expanded CD34+ and T and NK cells ex vivo in cord blood with different cytokines combination and transplanted into leukemic BALB/C nude mouse.

[Comparison between genotyping and serological phenotyping in RhCE blood group].

Objective: To genotype the RHCE gene of Hans, Xinjiang's Uigurs and Kazakstans in China, and to compare the results of RHCE genotyping with that of RhCc/Ee phenotyping.

Methods: RHCE genes of 98 Hans with RhD positive and 230 Hans, 72 Uigurs and 18 Kazakstans with RhD/RHD negative were genotyped with PCR-sequence specific primer (SSP) technique.

Results: The results of RHE/RHe genotyping from samples with RhD positive and negative were in accord with that of phenotyping.

[Determination of human RHD gene rhesus box and its significance].

The aim was to determine RHD zygosity, further to investigate genetic structure of RHD gene, and to predict hemolytic disease of newborn (HDN). The upstream box, downstream box, and hybrid box of RHD gene were determined by PCR-SSP with 4 primers under the same conditions. The results showed that only hybrid box could be determined in RHD(-)/RHD(-) homozygosity.

[Study on blood ABO typing in patients with autoimmune hemolytic anemia].

To explore effect of autoantibody on identification of ABO and RhD blood group, the blood samples of 38 patients with autoimmune hemolytic anemia (AIHA) were identified by routine typing and typing after chloroquine elution test as well as PCR. The results showed that out of 38 patients with AIHA, 11 cases (31.6%) of ABO blood group were difficulty typed, indirect antiglobulin test were positive, and contradiction between cells typing and sera typing were observed.

[Exon polymorphism of human RHD gene].

Objective: To study exon polymorphism of human RHD gene and investigate the genetic mechanism of RhD-negative individuals.

Methods: PCR using sequence-specific primers (PCR-SSP) was performed on 40 RhD-positive, 120 RhD-negative and 2 weak D blood samples.

Results: All 10 exons could be detected in the 40 RhD-positive and 2 weak D samples.

[Separation and cryopreservation of cord blood mononuclear cells].

The influencing factors on cord blood storage after collection and mononuclear cell separation as well as cryopreservation were studied. The mononuclear cell are separated from blood after blood collection, then cryopreserved and washed after thawed. Results showed that the cord blood kept at 4 degrees C or room temperature less than 24 hours after blood collection, mononuclear cell separated by hydroxyethylstarch and 2 centrifugations, mononuclear cell cryopreserved with 50% DMSO and autoplasma from cord blood as protectives and washing the cells after thawing.