Challenges in alpine meadow recovery: The minor effect of grass restoration on microbial resource limitation.

Microorganisms play a vital role in restoring soil multifunctionality and rejuvenating degraded meadows. The availability of microbial resources, such as carbon, nitrogen, and phosphorus, often hinders this process. However, there is limited information on whether grass restoration can alleviate microbial resource limitations in damaged slopes of high-altitude regions.

Discrimination of Benign and Malignant Thyroid Nodules through Comparative Analyses of Human Saliva Samples via Metabolomics and Deep-Learning-Guided Label-free SERS.

Thyroid nodules are a very common entity. The overall prevalence in the populace is estimated to be around 65-68%, among which a small portion (less than 5%) is malignant (cancerous). Therefore, it is important to discriminate benign thyroid nodules from malignant thyroid nodules.

Transcriptional regulation of daily sleep amount by TCF4-HDAC4-CREB complex in mice.

Histone deacetylase HDAC4/5 cooperates with cAMP response element-binding protein (CREB) in the transcriptional regulation of daily sleep amount downstream of LKB1-SIK3 kinase cascade in mice. Here, we report a significant enrichment of the E-box motifs for the basic loop-helix-loop (bHLH) proteins near the CREB- and HDAC4-binding sites in the mouse genome. Adeno-associated virus (AAV)-mediated expression of class I bHLH transcription factors, such as TCF4, TCF3, or TCF12, across the mouse brain neurons reduces the duration of rapid eye movement sleep (REMS) and non-REMS (NREMS).

Piezoresistive Sensor Based on Porous Sponge with Superhydrophobic and Flame Retardant Properties for Motion Monitoring and Fire Alarm.

Polyurethane sponge is frequently selected as a substrate material for constructing flexible compressible sensors due to its excellent resilience and compressibility. However, being highly hydrophilic and flammable, it not only narrows the range of use of the sensor but also poses a great potential threat to human safety. In this paper, a conductive flexible piezoresistive sensor (CHAP-PU) with superhydrophobicity and high flame retardancy was prepared by a simple dip-coating method using A-CNTs/HGM/ADP coatings deposited on the surface of a sponge skeleton and modified with polydimethylsiloxane.

Case report: Microsatellite instability-high pancreas adenosquamous carcinoma with postoperative liver metastasis recurrence treated with multimodality therapy achieving complete pathological response.

Pancreatic adenosquamous carcinoma (PASC) is a rare subtype of pancreatic cancer (PC), with no established consensus on the optimal treatment for postoperative liver metastasis recurrence. We report a case of a 48-year-old male patient who underwent radical surgery and was pathologically diagnosed with microsatellite instability-high (MSI-H) PASC. The patient experienced liver metastasis recurrence following single-agent gemcitabine adjuvant chemotherapy.

Placental and Fetal Microbiota in Rhesus Macaque: A Case Study Using Metagenomic Sequencing.

Recent evidence challenging the notion of a sterile intrauterine environment has sparked research into the origins and effects of fetal microbiota on immunity development during gestation. Rhesus macaques (RMs) serve as valuable nonhuman primate models due to their similarities to humans in development, placental structure, and immune response. In this study, metagenomic analysis was applied to the placenta, umbilical cord, spleen, gastrointestinal tissues of an unborn RM fetus, and the maternal intestine, revealing the diversity and functionality of microbes in these tissues.

Enhancing Early Breast Cancer Diagnosis With Contrast-Enhanced Ultrasound Radiomics: Insights From Intratumoral and Peritumoral Analysis.

Introduction: To develop and validate contrast-enhanced ultrasound (CEUS) radiomics model for the accurate diagnosis of breast cancer by integrating intratumoral and peritumoral regions.

Materials And Methods: This study enrolled 333 patients with breast lesions from Shenzhen people's hospital between March 2022 and March 2024. Radiomics features were extracted from both intratumoral and peritumoral (3 mm) regions on CEUS images.

Self-Cross-Linked Collagen Sponge from the Scale for Hemostasis and Wound Healing Applications.

Type I collagen, a crucial component maintaining the structural integrity and physiological function of various tissues, is widely regarded as one of the most suitable biomaterials for healthcare applications. In this study, shad scales, used for treating ulcers, scalds, and burns in traditional Chinese medicine, were exploited for type I collagen extraction. After self-assembly into hydrogels, the extracted collagen was subsequently freeze-dried to form collagen sponges.

Highly Efficient Synthesis of α-Amino Acids via Electrocatalytic C-N Coupling Reaction Over an Atomically Dispersed Iron Loaded Defective TiO.

The synthesis of α-amino acids via the electrocatalytic C-N coupling attracted extensive attention owing to the mild reaction conditions, controllable reaction parameters, and atom economy. However, the α-amino acid yield remains unsatisfying. Herein, the efficient electrocatalytic synthesis of α-amino acids is achieved with an atomically dispersed Fe loaded defective TiO monolithic electrocatalyst (Fe-TiO/Ti).

Clinical and genetic characteristics of a case of Koolen-De Vries syndrome caused by KANSL1 gene mutation and literature review: A case report.

Rationale: Koolen-De Vries syndrome (KdVS, OMIM: 612452), also known as 17q21.31 microdeletion syndrome, is an autosomal dominant genetic disease. In the study, we analyze of clinical phenotype and gene variation of a child with Koolen-De Vries syndrome, review the literature to improve the understanding of the disease.

Plasma-induced Fe-doped zeolitic imidazolate framework-8 derived P-Fe-NC for enhanced phenol degradation.

Plasma-synergistic catalysis is considered an effective method for degrading aromatic organic pollutants in water. However, the underlying synergistic catalytic mechanism between plasma and catalysts remains poorly understood. Here, we propose a plasma-metal organic frameworks (MOFs) synergistic strategy to investigate the mechanism of plasma-synergistic catalysts for phenol degradation.

Relationship between EZH2 expression and prognosis of patients with hepatocellular carcinoma using a pathomics predictive model.

Background: Enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) is overexpressed in hepatocellular carcinoma, promoting tumorigenesis and correlating with poor prognosis. Traditional histopathological examinations are insufficient to accurately predict hepatocellular carcinoma (HCC) survival; however, pathomics models can predict EZH2 expression and HCC prognosis. This study aimed to investigate the relationship between pathomics features and EZH2 expression for predicting overall survival of patients with HCC.

Identification of structural stability and fragility of mouse liver glycogen via label-free Raman spectroscopy coupled with convolutional neural network algorithm.

Glycogen structure is closely associated with its physiological functions. Previous studies confirmed that liver glycogen structure had two dominant states: mainly stable during the day and largely fragile at night. However, the diurnal change of glycogen structure is impaired, with dominant fragility in diseased conditions such as diabetes mellitus and liver fibrosis.

Artificial trimerization of β-glucosidase for enhanced thermostability and activity via computational redesign.

The improvement of enzyme thermostability is of great significance in the biotechnology industry. Herein, we modified the C-terminus of β-glucosidase via the computer-aided rational design and successfully obtained six variants that exhibit improved optimal temperatures and thermal inactivation half-lives at 65 °C. Among these variants, the thermal inactivation half-life and hydrolytic activity of TrCel1b-H13 at 65 °C were increased by 416 and 3223 folds, respectively, compared to those of the wild type.

Regulation of sleep amount by CRTC1 via transcription of in mice.

The cAMP-response element binding protein (CREB) is required for regulation of daily sleep amount, whereas gain-of-function of CREB-regulated transcription coactivator 1 (CRTC1) causes severe insomnia in mice. However, the physiological functions of CRTCs and their downstream target genes in the regulation of sleep amount remain unclear. Here, we use adult brain chimeric (ABC)-expression/knockout platform for somatic genetics analysis of sleep in adult male mice.

Piezoelectric nanocomposite electrospun dressings: Tailoring mechanics for scar-free wound recovery.

Rational wound management and enhancing healing quality are critical in clinical practice. Electrical stimulation therapy (EST) has emerged as a valuable adjunctive treatment due to its safety and cost-effectiveness. Integrating piezoelectric materials into dressings offers a way to miniaturize and personalize electrotherapy, enhancing convenience.

Combination of rituximab and low-dose glucocorticoids for idiopathic refractory nephrotic syndrome with MCD/FSGS: a single-center prospective cohort study.

Background: The treatment of idiopathic refractory nephrotic syndrome (IRNS) remains a difficult problem in clinical practice. This study aims to determine the efficacy and safety of combining low-dose glucocorticoids with rituximab in IRNS treatment.

Methods: This prospective, single-center cohort study enrolled 60 patients who were diagnosed with refractory IRNS with minimal change disease (MCD) or focal segmental glomerulosclerosis (FSGS) and treated at First Affiliated Hospital of Sun Yat-sen University.

The polysaccharide from purple sweet potato (Ipomoea batatas (L.) Lam) alleviates lipopolysaccharide-induced acute lung injury in mice via the VIP/cAMP/PKA/AQPs signaling pathway.

Background: The polysaccharide (PSP) from purple sweet potato has great potential for regulating apoptosis, but its regulatory role in acute lung injury (ALI) is unknown.

Methods: The objective of this study was to investigate the protective effect of PSP on lipopolysaccharide (LPS)-induced ALI in mice and lung epithelial A549 cells and its mechanism. Moreover, subacute toxicity evaluation of PSP was carried out on ICR mice.

Altitudinal decline of vegetation restoration effects on soil microbial communities on high-altitude roadside slops: Environmental drivers and management implications.

Construction activities in high-altitude regions have left many bare roadside slopes vulnerable to degradation, complicating restoration efforts. Soil microorganisms are vital for plant growth and nutrient cycling, yet their responses to restoration efforts at various altitudes remains uncertain. This study investigates soil microbial composition, network properties, ecological functions, keystone taxa, and environmental drivers across three restored vegetation types: herbaceous plants (H), shrubs + herbaceous plants (SH), and trees + shrubs + herbaceous plants (TSH) at elevations from 3100 to 3800 m.

SS-31 modification alleviates ferroptosis induced by superparamagnetic iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes.

Superparamagnetic iron oxide nanoparticles (SPION) are widely used in cardiovascular applications. However, their potential to induce ferroptosis in myocardial cells post-ischemia-reperfusion hinders clinical adoption. We investigated the mechanisms behind SPION-induced cytotoxicity in myocardial cells and explored whether co-loading SPION with SS-31 (a kind of mitochondrial-targeted antioxidant peptide) could counteract this toxicity.

Nanoparticle-mediated Klotho gene therapy prevents acute kidney injury to chronic kidney disease transition through regulating PPARα signaling in renal tubular epithelial cells.

Klotho is an anti-aging protein produced primarily by tubular epithelial cells (TECs). Down-regulated expression of Klotho in injured TECs plays a key pathogenic role in promoting acute kidney injury (AKI) to chronic kidney disease (CKD) transition, yet therapeutic approaches targeting the restoration of renal Klotho levels remain challenging for clinical application. Here, we synthesize polydopamine-polyethylenimine-l-serine-Klotho plasmid nanoparticles (PPSK NPs), which can safely and selectively deliver the Klotho gene to the injured TECs through binding kidney injury molecule-1 and maintain the expression of Klotho protein.