Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
April 2012
Aim: To construct the eukaryotic recombinant expression plasmid of pcDNA3.1(+)-PRMT1.
Methods: Human PRMT1 cDNA was amplified by reverse transcription polymerase chain reaction (RT-PCR).
Nan Fang Yi Ke Da Xue Xue Bao
April 2010
Objective: To observe the expression of protein arginine N-methyltransferase (PRMT) genes in the lung and spleen of E3 rats with acute asthma.
Methods: E3 rats with ovalbumin-induced pulmonary inflammation were divided into two groups (n=10), and the validity of the acute asthma model was evaluated by histological observation with HE and PAS staining and by measurement of NO production. Semi-quantitative RT-PCR was employed to detect the expressions of PRMT1-PRMT6 genes in the lung and spleen tissues of the rats.