[Application of double antibody sandwich ELISA for detection of nucleoside triphosphate hydrolase-II protein of Toxoplasma gondii].

Objective: To establish a double antibody sandwich ELISA method for detection of nucleoside triphosphate hydrolase-II (NTPase-II) protein of Toxoplasma gondii.

Methods: BALB/c mice were immunized with recombinant NTPase-II (rTgNTPase-II) protein of T. gondii.

[Microbial survey of bacterial contamination of shellfish products in Wenzhou].

Objective: To investigate the status of bacterial contamination in the shellfish products in Wenzhou.

Methods: One hundred samples were collected and their bacterial populations including the total plate count were investigated.

Results: Of the 100 samples collected, 67 samples failed to not meet the national regulations due to bacterial contamination, accounting for 67% of the total samples.

[Preparation, characterization and preliminary application of recombinant protein AP33 of trichomonas vaginalis].

Objective: To clone ap33 gene of Trichomonas vaginalis( T. v), construct prokaryotic expression system of the gene and identify its antigenicity and immunogenicity.

Methods: The total RNA was extracted from a clinical isolate Tv317 and the cDNA was synthesized by reverse transcription.