Talatisamine, a C(19)-diterpenoid alkaloid from Chinese traditional herbal 'Chuanwu'.

THE TITLE COMPOUND [SYSTEMATIC NAME: (1S,4S,5R,7S,8S,9R,10R,11S,13S,14S,16S,17R)-N-methyl-8,14-dihy-droxy-1,16-tri-meth-oxy-4-(meth-oxy-methyl-ene)aconitane], C(24)H(39)NO(5), was isolated from the roots of Aconitum carmichaelii Debx., which is known as 'Chuanwu' in Chinese traditional herbal medicine. The mol-ecule has an aconitane carbon skeleton with four six-membered rings and two five-membered rings, including a six-membered N-containing heterocyclic ring.

[Determination of diosgenin and ruscogenin in Radix Ophiopogonis by nonaqueous capillary electrophoresis].

Nonaqueous capillary electrophoresis is used for the determination of the contents of diosgenin and ruscogenin in Radix Ophiopogonis. The operating buffer was composed of 20 mmol x L(-1) Na2B4O7-HCl (pH 7.61) in 70% methanol.

[Experimental investigation of CAEP far-infra-red 100 microm FEL spontaneous emission].

In the FEL experiment, when electron beam passes through the wiggler, the movement of electron beam will be changed to wiggle in the wiggler due to the periodical magnet field. Meanwhile the electron beam will spontaneously emit the radiation with a wavelength similiar to the FEL radiation. This radiation is influenced by the parameters of both the electron beam and the wiggler.

[Research on the interaction of aristolochic acid and bovine serum albumin].

The interaction between aristolochic acid and bovine serum albumin (BSA) under physiological conditions was investigated by fluorescence quenching methods. The results indicate that there is a strong interaction between aristolochic acid and BSA, and the distances between the binding location and tryptophan residue is 2.8 nm.

[Simultaneous determination of the four effective components in Huaijiao pill by HPLC].

Objective: To develop an HPLC method for determination of the four effective components (genistin; rutin; quercetin; genistein) in Huaijiao pill.

Method: The chromatographic separation was performed on a Shim-packODS (4.6 mm x 150 mm, 5.

[Study of the methods of determining the isoflavones in herbs of Trifolium pratense extractive].

Objective: To study the methods of determining the amount of Isoflavones in red clover extractive.

Method: RP-HPLC is employed to determine the Isoflavones, with a C18 RP Column, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm.