RPA-CRISPR/Cas12a-LFA combined with a digital visualization instrument to detect in stray dogs and cats in Zhejiang province, China.

Unlabelled: , which causes toxoplasmosis, is prevalent in warm-blooded animals, such as cats, dogs, and humans. causes economic losses to livestock production and represents a potential risk to public health. Dogs and cats are common hosts in the epidemiology of toxoplasmosis.

Genome-wide miRNAs expression profiles of Schistosoma japonicum schistosomula in response to artesunate.

Aim: miRNAs play a significant role in pharmacogenomics and are likely to be important in the molecular mechanism of atesunate (ART) effects on Schistosoma japonicum.

Methods: We sequenced the RNAs using an Illumina (Solexa) DNA sequencer and compared the relative expression levels of the miRNAs in 10-day-old schistosomula from ART and the parallel control group.

Results: We characterized 95 known miRNAs from S.

[Research of Straw Biomass Based on NIR by Wavelength Selection of IPLS-SPA].

The whole spectrum usually contains a lot of redundant information in the near-infrared spectroscopy model, the presence of redundant information will increase the model resolution time and increase the difficulty of parsing model, Therefore, how to select the characteristic wavelength quickly and effectly is very crucial. In this paper, we combined the algorithm based on SPA (successive projections algorithm ) with IPLS (interval partial least squares ) to selec the characteristic wavelength in the fermentation of wheat straw microbial biomass, A total of 85 samples prepared by measuring microbial biomass using glucosamine method, 68 samples are chosen as calibration set and 17 simples are chosen as verification set. First, the whole spectral region 520 points are segmented modeling according to the interval wavelength point size 10, 20, 30, 40 and 4 450~4 925 cm-1, 9 194~9 993 cm-1 two-band range are selected as the characteristic wavelength band, then pick out the new feature wavelength points by Successive Projections Algorithm band and Genetic Algorithm (GA), comprehensive analysis and comparison the result of model.

Quantitative proteomic analyses of Schistosoma japonicum in response to artesunate.

Artesunate (ART) has high prophylactic efficacy against Schistosoma japonicum infections and has been used to treat and prevent schistosomiasis in China since 1995. However, the molecular mechanism of ART's effects on S. japonicum remains unclear.

[Rapid discriminating hogwash oil and edible vegetable oil using near infrared optical fiber spectrometer technique].

In the present study, a new method using near infrared spectroscopy combined with optical fiber sensing technology was applied to the analysis of hogwash oil in blended oil. The 50 samples were a blend of frying oil and "nine three" soybean oil according to a certain volume ratio. The near infrared transmission spectroscopies were collected and the quantitative analysis model of frying oil was established by partial least squares (PLS) and BP artificial neural network The coefficients of determina- tion of calibration sets were 0.

Loop-mediated isothermal amplification (LAMP): early detection of Toxoplasma gondii infection in mice.

Background: Toxoplasmosis is a widespread zoonotic parasitic disease that occurs in both animals and humans. Traditional molecular assays are often difficult to perform, especially for the early diagnosis of Toxoplasma gondii infections. Here, we established a novel loop-mediated isothermal amplification targeting the 529 bp repeat element (529 bp-LAMP) to detect T.

Loop-mediated isothermal amplification: rapid detection of Angiostrongylus cantonensis infection in Pomacea canaliculata.

Background: Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. The most common source of infection with A. cantonensis is the consumption of raw or undercooked mollusks (e.