[The cloning of mouse FasL gene and expression in chondrocytes].

Aim: To clone mFasL cDNA, then insert it into retrovirus expression system pLNCX(2), and express mFasL cDNA in chondrocytes to study its function.

Methods: RT-PCR was applied to amplify mFasL cDNA from the total RNA of activated mouse spleen lymphocytes. The cDNA was inserted into a T-cloning vector, then subcloned into pLNCX(2).

[The experimental study of tissue engineered autologous cartilage using chitosan-gelatin complex scaffolds].

Objective: To investigate whether man-made porous chitosan-gelatin complex scaffold was a appropriate scaffold for tissue engineering cartilage.

Methods: Chondrocytes isolated from Changfeng crossbred swines' auricular cartilage were seeded onto chitosan- gelatin scaffolds to be cultured in a three dimensional environment. The chondrocyte- polymer constructs were implanted into the subcutaneous tissue of the swines' abdomenal wall.

Repression of allo-cell transplant rejection through CIITA ribonuclease P+ hepatocyte.

Aim: Allo-cell transplant rejection and autoimmune responses were associated with the presence of class II major histocompatibility complex (MHC II) molecules on cells. This paper studied the effect of Ribonuclease P (RNase P) against CIITA, which was a major regulator of MHCII molecules, on repressing the expression of MHCII molecules on hepatocyte.

Methods: M1-RNA is the catalytic RNA subunit of RNase P from Escherichia coli.

[Overexpression of truncated type II transforming growth factor-beta receptor in dermal fibroblasts].

Objective: To study the effect of overexpression of truncated type II TGF-beta receptor on transforming growth factor-beta 1(TGF-beta 1) autoproduction in normal dermal fibroblasts.

Methods: In vitro cultured dermal fibroblasts were treated with recombinant human TGF-beta 1(rhTGF-beta 1) (5 ng/ml) or recombinant adenovirus containing truncated type II TGF-beta receptor gene (50 pfu/cell). Their effects on regulating gene expression of TGF-beta 1 were observed with Northern blotting.