[Comparison of properties of Mahuang decoction and Maxing Shigan decoction based on biothermodynamics].

Objective: To probe into the objectivity and authenticity of four properties beteen Mahuang decoction and Maxing Shigan decoction from biothermodynamics.

Method: The power-time curves of growth of Staphylococcus aureus at different concentrations between Mahuang decoction and Maxing Shigan decoction were determined by TAM Air Isothermal Calorimeter. The growth rate constants of promotive and inhibitory actions were calculated.

[Fingerprints of different samples of processed Rhizoma Coptidi by HPLC].

Objective: To set up the HPLC fingerprints of different samples of processed Rhizoma Coptidi (RC) in order to offer evidence for identifying different processed RC and preliminary discussion on processing mechanism.

Method: Seven different samples of processed RC were determined by HPLC and the results were analyzed by Hierarchical clustering and similarity evaluation system for chromatographic fingerprint of TCM.

Result: The characteristic of common peaks for fingerprints of different samples of processed RC is distinct, but there are differences among various processed RC.

[The determination of contents of 8 ginsenosides in extraction of Panax ginseng by HPLC].

Objective: To establish a method to determine the contents of 8 ginsenosides in extraction of Panax ginseng by HPLC.

Methods: The sample was analyzed on an ODS chromatogram column (Kromasil 250 mm x 4.6 mm, 5 microm), with mobile phase of acetonitrile-water (gradient elution) at flow rate 1.

[Determination of total organic acids and salicylic acid in extract of Radix isatidis].

Objective: To develop a quantitative method for determination of the total organic acids and salicyclic acid in the extract of Radix Isatidis.

Method: The total organic acids were determined by acid-base titration and the salicylic acid was determined by HPLC.

Result: It was shown that contents of total organic acids and salicylic acid in the extract of Radix Isatidis were 13.

[Effects of different extracts from Radix isatidis on lymphocytes of mice by biothermodynamics].

Objective: To observe the different extracts from Radix Isatidis on multiplication of mice lymphocytes.

Method: Lymphocytes were separated and cultured. Immunological activities of different extracts from Radix Isatidis were studied by thermodynamics and the results were tested by the conventional pharmacological experiments.

Thermodynamic study on antibacterial effect of different extracts from Radix Isatis.

Objective: To study and analyze the antibacterial effects of different extracts from Radix Isatis.

Methods: Staphylococcus aureus was used as the studied object in the experiment. Antibacterial effects of extracts from Radix Isatis were observed by thermocalrimetry on Staphylococcus aureus, together with common pharmacological experiments.