Publications by authors named "Qing-Man Wang"
There is broad consensus that RecQ family helicase is a high-order oligomer that dissociates into a dimer upon ATP binding. This conclusion is based mainly on studies of highly purified recombinant proteins, and the oligomeric states of RecQ helicases in living cells remain unknown. We show here that, in contrast to current models, monomeric RECQL helicase is more abundant than oligomer/dimer forms in living cells.
View Article and Find Full Text PDFArticle Synopsis
- RPA is a crucial protein that binds to single-stranded DNA (ssDNA) and plays a vital role in maintaining genomic stability by engaging in dynamic interactions with ssDNA of varying lengths and structures.
- The research utilized single-molecule FRET techniques to reveal how RPA forms different binding complexes with ssDNA, leading to changes in the ssDNA's shape, influenced by the DNA length, structure, and RPA concentration.
- The findings suggest that while RPA binds more strongly to ssDNA than RNA, it still engages in rapid binding and unbinding with RNA, highlighting the complex dynamics of RPA's interactions with both nucleic acids.
G-quadruplexes (G4s) are four-stranded DNA structures formed by Hoogsteen base pairing between stacked sets of four guanines. Pif1 helicase plays critical roles in suppressing genomic instability in the yeast by resolving G4s. However, the structural properties of G4s in and the substrate preference of Pif1 for different G4s remain unknown.
View Article and Find Full Text PDF