Investigation of dental pulp stem cells isolated from discarded human teeth extracted due to aggressive periodontitis.

Recently, human dental pulp stem cells (DPSCs) isolated from inflamed dental pulp tissue have been demonstrated to retain some of their pluripotency and regenerative potential. However, the effects of periodontal inflammation due to periodontitis and its progression on the properties of DPSCs within periodontally compromised teeth remain unknown. In this study, DPSCs were isolated from discarded human teeth that were extracted due to aggressive periodontitis (AgP) and divided into three experimental groups (Groups A, B and C) based on the degree of inflammation-induced bone resorption approaching the apex of the tooth root before tooth extraction.

[Cloning and transcriptional regulation analysis of amelogenin promoter in different cells].

Purpose: To clone different promoter region of amelogenin gene and analyze their transcriptional activity.

Methods: The upstream regulation sequences of amelogenin gene were retrieved and analyzed. Amplified by PCR from the genomic DNA of mouse C57BL/6J and digested with restriction endonucleases enzyme, different transcriptional regulation sequences of 5'flanking of amelogenin gene including the basal promoter were cloned and ligated with luciferase gene in PGL3-Basic vector.

[Cloning and sequencing of the upstream of mouse dentin sialophosphoprotein promoter].

Objective: To clone and sequence the upstream of mouse dentin sialophosphoprotein promoter.

Methods: Genomic DNA was obtained from Balb/c mouse blood. The upstream of mouse dentin sialophosphoprotein promoter segments was obtained by PCR.

[Cbfa1 induces the expression of the mineral-related proteins in human dental papilla cells].

Objective: To explicit whether the expression of the mineral-related proteins is regulated by cbfa1 in human dental papilla cells.

Methods: Human dental papilla cells were cultured in vitro and transfected with pcDNA3-cbfa1 recombinant plasmids. After selected with G418 sulfate, a cell clone named PC-3, which could stably express the cbfa1 mRNA and protein, was proved by PCR and western blot.