Efficient markerless gene deletions in Pseudomonas protegens Pf-5 using a upp-based counterselective system.

Objectives: Developing a counterselective system for efficient markerless gene deletions in biocontrol strain P. protegens Pf-5.

Results: We successfully implemented a markerless deletion of upp in Pf-5 to obtain the 5-FU resistant strain Pf5139.

[Clinical diagnosis and treatment of superior tibial fracture complicating with crotch injury of distal popliteal artery].

Objective: To investigate the clinical effects of treatment for superior tibial fracture complicating with crotch injury of distal popliteal artery by an alternative micro-surgical operation.

Methods: During Feb. 2002 to Oct.