[Cloning, sequencing and subcloning of cDNA coding for group I allergen of Dermatophagoides farinae].

Objective: To clone, sequence and subclone the cDNA coding for group 1 allergen of Dermatophagoides farinae (Der f 1).

Methods: The cDNA of Der f 1 was amplified by RT-PCR and PCR. After purified, the gene fragment was cloned into a vector pMD-18T.

Diarrhea and acaroid mites: a clinical study.

Aim: To explore the characteristics of diarrhea caused by acaroid mites.

Methods: Acaroid mites in fresh stools of 241 patients with diarrhea were separated by flotation in saturated saline. Meanwhile, skin prick test, total IgE and mite-specific IgE were detected in all patients.

Acaroid mite, intestinal and urinary acariasis.

Aim: To investigate epidemiology and pathogenic mite species of intestinal and urinary acariasis in individuals with different occupations.

Methods: A total of 1994 individuals were tested in this study. History collection, skin prick test and pathogen identification were conducted.

L-forms of H. pylori.

Aim: To study the occurrence of L-forms of H. pylori infection in patients with peptic ulcers and its association with possible changes of cellular immune function in the patients.

Methods: Endoscopic biopsy specimens of gastric antrum and gastric corpus were taken from 228 patients with peptic ulcers and inoculated into Skirrow selective medium for H.