NIR-715 photodynamic therapy induces immunogenic cancer cell death by enhancing the endoplasmic reticulum stress response.

Effectively interfering with endoplasmic reticulum (ER) function in tumor cells and simultaneously activating an anti-tumor immune microenvironment to attack the tumor cells are promising strategies for cancer treatment. However, precise ER-stress induction is still a huge challenge. In this study, we synthesized a near-infrared (NIR) probe, NIR-715, which induces tumor cell death and inhibits tumor growth without causing apparent side effects.

Lympho-myeloid aggregate-infiltrating CD20 B cells display a double-negative phenotype and correlate with poor prognosis in esophageal squamous cell carcinoma.

According to morphological features, tumor-infiltrating B cells (TIL-Bs) can be classified as lympho-myeloid aggregates (LMAs) and tertiary lymphoid structures (TLSs). As a disease with high incidence and mortality, research on esophageal squamous cell carcinoma (ESCC) TIL-Bs is still unclear. Thus, we aimed to investigate the prognostic value and functional involvement of TIL-Bs in ESCC.

KAT8/SIRT7-mediated Fascin-K41 acetylation/deacetylation regulates tumor metastasis in esophageal squamous cell carcinoma.

Fascin actin-bundling protein 1 (Fascin) is highly expressed in a variety of cancers, including esophageal squamous cell carcinoma (ESCC), working as an important oncogenic protein and promoting the migration and invasion of cancer cells by bundling F-actin to facilitate the formation of filopodia and invadopodia. However, it is not clear how exactly the function of Fascin is regulated by acetylation in cancer cells. Here, in ESCC cells, the histone acetyltransferase KAT8 catalyzed Fascin lysine 41 (K41) acetylation, to inhibit Fascin-mediated F-actin bundling and the formation of filopodia and invadopodia.

Spatial analysis of stromal signatures identifies invasive front carcinoma-associated fibroblasts as suppressors of anti-tumor immune response in esophageal cancer.

Background: Increasing evidence indicates that the tumor microenvironment (TME) is a crucial determinant of cancer progression. However, the clinical and pathobiological significance of stromal signatures in the TME, as a complex dynamic entity, is still unclear in esophageal squamous cell carcinoma (ESCC).

Methods: Herein, we used single-cell transcriptome sequencing data, imaging mass cytometry (IMC) and multiplex immunofluorescence staining to characterize the stromal signatures in ESCC and evaluate their prognostic values in this aggressive disease.

Identification of hub biomarkers and immune cell infiltration characteristics of polymyositis by bioinformatics analysis.

Background: Polymyositis (PM) is an acquirable muscle disease with proximal muscle involvement of the extremities as the main manifestation; it is a category of idiopathic inflammatory myopathy. This study aimed to identify the key biomarkers of PM, while elucidating PM-associated immune cell infiltration and immune-related pathways.

Methods: The gene microarray data related to PM were downloaded from the Gene Expression Omnibus database.

Circular RNA Sirtuin1 represses pulmonary artery smooth muscle cell proliferation, migration and autophagy to ameliorate pulmonary hypertension via targeting microRNA-145-5p/protein kinase-B3 axis.

Recently, several studies have been clarified that circular RNA (circRNA) was a vital regulatory gene of pulmonary hypertension (PH). Nevertheless, the action of circRNA in PH was not yet explored. This study was to figure out the biological function and potential molecular mechanism of circSirtuin1 (SIRT1) in PH.

Integrated single-cell transcriptome analysis reveals heterogeneity of esophageal squamous cell carcinoma microenvironment.

The tumor microenvironment is a highly complex ecosystem of diverse cell types, which shape cancer biology and impact the responsiveness to therapy. Here, we analyze the microenvironment of esophageal squamous cell carcinoma (ESCC) using single-cell transcriptome sequencing in 62,161 cells from blood, adjacent nonmalignant and matched tumor samples from 11 ESCC patients. We uncover heterogeneity in most cell types of the ESCC stroma, particularly in the fibroblast and immune cell compartments.

Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.

Esophageal cancer (EC) is a type of aggressive cancer without clinically relevant molecular subtypes, hindering the development of effective strategies for treatment. To define molecular subtypes of EC, we perform mass spectrometry-based proteomic and phosphoproteomics profiling of EC tumors and adjacent non-tumor tissues, revealing a catalog of proteins and phosphosites that are dysregulated in ECs. The EC cohort is stratified into two molecular subtypes-S1 and S2-based on proteomic analysis, with the S2 subtype characterized by the upregulation of spliceosomal and ribosomal proteins, and being more aggressive.

Clinical Characteristics of Children with Coronavirus Disease 2019 in Hubei, China.

Since December 2019, COVID-19 has occurred unexpectedly and emerged as a health problem worldwide. Despite the rapidly increasing number of cases in subsequent weeks, the clinical characteristics of pediatric cases are rarely described. A cross-sectional multicenter study was carried out in 10 hospitals across Hubei province.

[Application of residual crown of premolar restored with two shapes of fiber post systems].

Purpose: To compare the effects of premolars restored with 2 different types of fiber post systems, so as to provide experience for restoration of residual crown of premolars.

Methods: Fifty-three residual crowns of premolar restored with fiber post systems were collected, and randomly divided into 2 groups: parallel fiber post group and double taper fiber post group. Repairing effect and operation difficulty were compared.

Fluorescence-guided resection of high-grade gliomas: a systematic review and meta-analysis.

Objective: The present systematic review and meta-analysis was to analyze of the advantages of intraoperative fluorescence-guided resection of high-grade gliomas.

Methods: Systematic computerized searches of the PubMed and Web of Knowledge were performed. The outcomes included diagnostic value for identification of tumor tissue, gross total resection, and prognosis.

Corrosion behavior of pure titanium in the presence of Actinomyces naeslundii.

It is well known that some microorganisms affect the corrosion of dental metal. Oral bacteria such as Actinomyces naeslundii may alter the corrosion behavior and stability of titanium. In this study, the corrosion behavior of titanium was studied in a nutrient-rich medium both in the presence and the absence of A.

Bacterial diversity of subgingival plaque in 6 healthy Chinese individuals.

The subgingival microbial ecology is complex, and little is known regarding its bacteria species composition in healthy Chinese individuals. This study aimed to identify the subgingival microbiota from 6 healthy Chinese subjects. Subgingival samples from 6 volunteers were collected, the 16S rRNA gene was amplified using broad-range bacterial primers, and clone libraries were constructed.

Hemi-semi laminectomy approach for the microsurgical treatment of spinal schwannomas.

Objective: To evaluate the safety and efficiency of hemi-semi laminectomy approach for the microsurgical treatment of spinal schwannomas.

Methods: A total of 22 patients underwent hemi-semi laminectomy for the microsurgical removal of spinal schwannomas during a period of 2009 and 2011 in Affiliated Hospital of Nantong University. We retrospectively analyzed the clinical outcomes of these patients.

[A new method of fabricating photoelastic model by rapid prototyping].

Purpose: To explore a novel method of fabricating the photoelastic model using rapid prototyping technique.

Methods: A mandible model was made by rapid prototyping with computerized three-dimensional reconstruction, then the photoelastic model with teeth was fabricated by traditional impression duplicating and mould casting.

Results: The photoelastic model of mandible with teeth, which was fabricated indirectly by rapid prototyping, was very similar to the prototype in geometry and physical parameters.

[Three-dimensional finite element analysis of the influence of implant location on the stress distribution for implant-supported fixed partial dentures].

Purpose: To evaluate the influence of implant location on the stress distribution for implant-supported fixed partial dentures by 3-D finite element analysis.

Methods: Four 3-D finite element models with different implant location were constructed. Four different loading conditions(vertical distributed,oblique distributed,vertical concentrated,oblique concentrated) were applied to investigate the stress distribution by finite element analysis software ABAQUS 6.

[The effects of different welding wires on the mechanical properties of laser welding joints].

Purpose: To evaluate the mechanical properties and microstructure of laser-welded joints with different welding wires for clinical use of welding wire.

Methods: The standard tensile test and three-point bending test rods were made from Co-Cr and Ni-Cr alloy, and were laser-welded with different welding wire (commercially welding wire and casting wire). Then the tensile rods were tested for the ultimate tensile strength (UTS), and the bending rods for the ultimate bending strength (UBS).

[The mechanic retention of the casting post with keeper in magnetic retentor overdenture].

Purpose: To reduce the cost of the fabrication of the magnetic retentor,and meet the clinical requirements, nonprecious alloy such as Co-Cr alloy was used to fabricate the magnetic retentor in overdenture , instead of the precious alloy.

Methods: A mandibular canine was chosen for root canal preparation in vitro. Thirty two resin posts were made in the same root canal with self-curing resin.

[Clinical observation of treating nonrestorable teeth with orthodontics, periodontal therapy and prosthodontics].

Purpose: To evaluate the clinical results of treating nonrestorable teeth with conjunctive efforts of orthodontic-prosthodontic disciplines and periodontal therapy.

Methods: 10 cases, 8 anterior teeth, 2 premolar were selected, with a large vertical root fracture lingually or labially extending 2 to 3 mm below the gingival margin. The teeth were erupted coronally by orthodontic forces, and stabilized by the appliances for 8 weeks to 6 months, then periodontal surgery was performed, at last the final restoration was completed.

[Effect of laser irradiation power on the mechanical properties of Co-Cr and Ni-Cr laser-welded joints.].

Purpose: To investigate the effect of different irradiation power on the mechanical properties of laser-welded joints.

Methods: The standard tensile test and three-point bending test rods made from Co-Cr and Ni-Cr alloy were laser-welded with different irradiation powers. Then the tensile rods were tested for the ultimate tensile strength (UTS), and the bending rods for the ultimate bending strength (UBS).

[An experimental study on laser-welded dissimilar alloys in dentistry.].

Purpose: This study evaluated the mechanical properties and microstructure of the joints of laser-welded dissimilar alloys usually used in clinical nowadays.

Methods: The dissimilar alloys for Co-Cr alloy,Ni-Cr alloy and pure titanium were laser-welded. The joints were tested for the ultimate tensile strength (UTS) and the ultimate bending strength (UBS).

[Effect of irradiation power on the mechanical properties of laser-welded titanium joints.].

Purpose: To make an clinical implication for the use of laser welding pure titanium,this study investigate the effect of irradiation power on the mechanical properties of laser-welded joints.

Methods: The pure titanium tensile test and three-point bending test rods were laser-welded with different irradiation power. Then the tensile rods were tested for the ultimate tensile strength (UTS), and the bending rods for the ultimate bending strength (UBS).