[Development of SNP multiplexes utilizing universal reporter primers for forensic purposes].

Objective: To establish a simple, fast and economical technique for multiplex-typing SNPs and to explore its potential forensic application.

Methods: Five Y-SNP loci (IMS-JST164520, IMS-JST021354, IMS-JST003305, M119 and M134) were selected and the allele specific primers of each locus were designed with the universal reporter primers tailed at their 5' end. Alleles of these loci were amplified first by allele specific primers, then amplified by universal reporter primers tagged by fluorescent dye.

[The application of PCR-SSCP in forensic mtDNA typing].

Objective: To study the application of PCR-SSCP in forensic mtDNA typing.

Methods: Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.

[Applications of DNA methylation markers in forensic medicine].

DNA methylation is a post-replication modification that is predominantly found in cytosines of the dinucleotide sequence CpG. Epigenetic information is stored in the distribution of the modified base 5-methylcytosine. DNA methylation profiles represent a more chemically and biologically stable source of molecular diagnostic information than RNA or most proteins.

[A simple and rapid modified--new method for SNP typing by fragment length discrepant allele specific PCR].

Objective: To establish a new method for single nucleotide polymorphism (SNP) typing based on allele specific PCR: fragment length discrepant allele specific PCR (FLDAS-PCR), and study the influence on specific extension by introducing a mismatch at the third or fourth 3'-terminal base of allele specific primers.

Methods: For SNP loci rs759117 and rs760887, two allele specific forward primers, with different length and a mismatch introduced at the third or fourth 3'-terminal base, and a public reverse primer were designed for SNP typing. The genotyping of SNP was determined by the two allele specific fragments different in size after polyacrylamide gel and silver staining.

[Application of the age-associated injure in mitochondrial DNA].

Nowadays, the injury in human mitochondrial DNA (mtDNA) is well known to accumulate in various tissues with age. It's significant to further investigate and then apply it to estimation of the age at parenchymas.

[Application of chi-square test and exact test in Hardy-Weinberg equilibrium testing].

This article review the application of chi-square test of various data handling methods and exact test in Hardy-Weinberg equilibrium testing of human genetic marker in population genetics. The importance of HWE-exact test in multiallelic system was emphasized, especially in the study of forensic VNTR and STR typing.

[PGM1 genotyping by PCR-SSCP].

Objective: PGM1 genotyping by PCR-SSCP analysis.

Methods: Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes.

Results: 2 alleles and 3 genotypes were detected in exon 4 and 8 respectively.

[A study of genetic polymorphism of the STR loci D20S85 and D6S477 in Han population living in Wuhan].

Objective: The genetic polymorphism of two STR loci, D20S85 and D6S477, were studied in 280 unrelated Chinese individuals in Wuhan.

Methods: The PCR amplified products were analyzed by PAGE and silver staining.

Results: 10 and 9 alleles were observed in these two STR loci, and the discriminating power (DP) were 0.