Publications by authors named "Qing-Bing Zheng"
Background & Aims: HBV expresses more than 10 spliced RNAs from the viral pregenomic RNA, but their functions remain elusive and controversial. To address the function of HBV spliced RNAs, we generated splicing-deficient HBV mutants and conducted experiments to assess the impact of these mutants on HBV infection.
Methods: HepG2-NTCP cells, human hepatocyte chimeric FRG mice (hu-FRG mice), and serum from patients with chronic hepatitis B were used for experiments on HBV infection.
Machine Learning for Structure Determination in Single-Particle Cryo-Electron Microscopy: A Systematic Review.
IEEE Trans Neural Netw Learn Syst
February 2022
Recently, single-particle cryo-electron microscopy (cryo-EM) has become an indispensable method for determining macromolecular structures at high resolution to deeply explore the relevant molecular mechanism. Its recent breakthrough is mainly because of the rapid advances in hardware and image processing algorithms, especially machine learning. As an essential support of single-particle cryo-EM, machine learning has powered many aspects of structure determination and greatly promoted its development.
View Article and Find Full Text PDFObjective: This study aimed to develop a novel therapeutic vaccine based on a unique B cell epitope and investigate its therapeutic potential against chronic hepatitis B (CHB) in animal models.
Methods: A series of peptides and carrier proteins were evaluated in HBV-tolerant mice to obtain an optimised therapeutic molecule. The immunogenicity, therapeutic efficacy and mechanism of the candidate were investigated systematically.
Article Synopsis
- The study investigates mutations in the hepatitis B surface protein in individuals with occult hepatitis B virus infection and their effect on viral factors.
- It analyzes 61 occult HBV carriers and compares them with two other groups based on HBsAg levels, identifying mutations in the major hydrophilic region (MHR) of HBV.
- Results show that a significant proportion of occult carriers have MHR mutations that reduce antigen detection and impair protein secretion, which may lead to challenges in diagnosing the infection.
Background: Rapid influenza A diagnostic tests (RIDTs) play an important role in the clinical setting, especially in the influenza post-pandemic era with three influenza A viruses in circulation.
Objectives: Determine the sensitivity and specificity of a new RIDT (FluA Dot) by comparison with BD Directigen EZ FluA+B and CDC rRT-PCR.
Study Design: Two sets of experiments were conducted to determine the performance of the new test.