A Microfluidic Biosensor Based on Magnetic Nanoparticle Separation, Quantum Dots Labeling and MnO Nanoflower Amplification for Rapid and Sensitive Detection of Typhimurium.

Screening of foodborne pathogens is an effective way to prevent microbial food poisoning. A microfluidic biosensor was developed for rapid and sensitive detection of Typhimurium using quantum dots (QDs) as fluorescent probes for sensor readout and manganese dioxide nanoflowers (MnO NFs) and as QDs nanocarriers for signal amplification. Prior to testing, amino-modified MnO nanoflowers (MnO-NH NFs) were conjugated with carboxyl-modified QDs through EDC/NHSS method to form MnO-QD NFs, and MnO-QD NFs were functionalized with polyclonal antibodies (pAbs) to form MnO-QD-pAb NFs.

An untargeted and pseudotargeted metabolomic combination approach to identify differential markers to distinguish live from dead pork meat by liquid chromatography-mass spectrometry.

An untargeted and pseudotargeted metabolomic combination approach was developed to identify reliable and stable differential markers which can distinguish between pork meat from live pigs conventionally butchered and pork meat from dead pigs butchered immediately after death from diseases or other abnormalities. In this study, 24 differential metabolites of interest were screened by the UHPLC-Triple-TOF-MS-based untargeted metabolomic method, and 14 differential markers were detected by the UHPLC-QTRAP-MS-based pseudotargeted metabolomic method after performing statistical analysis to remove false-positive differential metabolites. Among the possible differential markers identified using the Metlin database and references were carnosine, l-carnitine, l-histidine, N-acetylhistidine, acetylcholine, l-acetylcarnitine and two phosphatidylcholines.

An exo probe-based recombinase polymerase amplification assay for the rapid detection of porcine parvovirus.

Recombinase polymerase amplification (RPA), an isothermal amplification technology, has been developed as an alternative to PCR in pathogen detection. A real-time RPA assay (rt-RPA) was developed to detect the porcine parvovirus (PPV) using primers and exo probe specific for the VP2 gene. The amplification was performed at 39°C for 20min.

Reverse transcription recombinase polymerase amplification assay for the rapid detection of type 2 porcine reproductive and respiratory syndrome virus.

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important pathogens in pigs, and has tremendous negative economic impact on the swine industry worldwide. PRRSV is classified into the two distinct genotypes: type 1 and type 2, and most of the described PRRSV isolates in China are type 2. Rapid and sensitive detection of PRRSV is of great importance for the disease control and regional eradication programs.