A proteomic signature for human papillomavirus-associated oropharyngeal squamous cell carcinoma predicts patients at high risk of recurrence.

Although HPV-positive OPSCC is associated with better prognosis than HPV-negative disease, ~30% of cases relapse despite curative-intent radiotherapy (+/- chemotherapy). We aimed to develop a proteomic signature associated with risk of recurrence within HPV+OPSCC. We analysed tumor specimens from 124 patients with T1-4N0-3M0 HPV+OPSCC: 50 patients with residual or recurrent disease within 5 years of treatment and 74 age and performance-status matched patients with no recurrence.

Decoding SFRP2 progenitors in sustaining tooth growth at single-cell resolution.

Background: Single-cell transcriptomics has revolutionized tooth biology by uncovering previously unexplored areas. The mouse is a widely used model for studying human tissues and diseases, including dental pulp tissues. While human and mouse molars share many similarities, mouse incisors differ significantly from human teeth due to their continuous growth throughout their lifespan.

A novel ypN-TRG staging system for gastric cancer patients after neoadjuvant therapy based on the metro-ticket paradigm: a multicenter and large sample retrospective analysis.

Background: Conventional ypT category evaluates the depth of invasion after neoadjuvant therapy (NAT) for gastric cancer (GC) and has limited prognostic value. Tumor regression grade (TRG) measures the extent of tumor response to treatment, and when combined with the ypN category, it may enhance the prediction of patient outcomes. This study aims to develop a new staging system by integrating TRG and ypN category to better evaluate the prognosis of GC patients receiving NAT.

Persistent activation of TRPM4 triggers necrotic cell death characterized by sodium overload.

Sodium influx and overload are frequently observed in human tissue injuries. Whether sodium overload imposes a causative effect on necrotic cell death and the mechanism involved are unclear. Here we identify necrocide 1 (NC1) as a compound that induces necrotic cell death through sodium overload, termed NECSO for necrosis by sodium overload.

The effect of storage time and temperature on the proteomic analysis of FFPE tissue sections.

Formalin-fixed paraffin-embedded (FFPE) tissues present an important resource for cancer proteomics. They are more readily available than fresh frozen (FF) tissues and can be stored at ambient temperature for decades. FFPE blocks are largely stable for long-term preservation of tumour histology, but the antigenicity of some proteins in FFPE sections degrades over time resulting in deteriorating performance of immunohistochemistry (IHC).

Blocking MIF secretion enhances CAR T-cell efficacy against neuroblastoma.

Introduction: Chimeric antigen receptor (CAR) T-cell therapy is a promising and innovative cancer therapy. However, immunosuppressive tumor microenvironments (TME) limit T cell persistence and durable efficacy. Here, we aimed to identify and target immunosuppressive factors in the TME of neuroblastoma, a pediatric extracranial solid tumor, to improve CAR-T efficacy.

Comparison of a submucosal and subserosal approach in ICG-guided laparoscopic lymphadenectomy in gastric cancer patients: long-term outcomes of a phase 3 randomized clinical trial.

Background: Previous studies have demonstrated similar short-term efficacy between subserosal (SSA) and submucosal (SMA) approaches for ICG injection in gastric cancer (GC). This study aims to compare the long-term oncological outcomes of these two injection methods for lymph node (LN) tracing in ICG-guided laparoscopic gastrectomy.

Materials And Methods: This study was a phase 3, open-label, randomized clinical trial (FUGES-019).

Covert chaotic encryption scheme based on compressive sensing in a W-band RoF system.

This paper proposes a covert chaotic encryption (CCE) scheme based on compressive sensing (CS). The chaotic sequences used are generated by a six-dimensional hyper-chaotic D-system, where the sequence is utilized for a chaotic index sparse block (CISB), the sequence is used for generating the CS measurement matrix effectively, the , , and sequences are employed for variable-parameter iterative Arnold transformations, and the sequence is used for dual-random least significant bit (LSB) scrambling and embedding. The combination of these technologies enabled the scheme to achieve multi-domain, multi-dimensional, ultra-high-security encryption for multimedia image data.

Characterization changes and research waste in randomized controlled trials of global gastroesophageal reflux disease and hiatus hernia over the past 20 years.

Background: The results of many large randomized clinical trials (RCTs) have transformed clinical practice in gastroesophageal reflux disease (GERD) and esophageal hiatal hernia (HH). However, research waste (i.e.

DDX21 Is a Potential Biomarker for Predicting Recurrence and Prognosis in Hepatocellular Carcinoma.

DEAD-box helicase 21 (DDX21) is a conserved Asp-Glu-Ala-Asp (DEAD) box RNA helicase with multiple functions that is involved in various cellular processes and diseases. However, the role of DDX21 in the recurrence and prognosis of hepatocellular carcinoma (HCC) patients remains unknown. In the current study, we examined the protein expression of DDX21 in HCC tissues through immunohistochemical staining and analyzed the correlation between DDX21 protein expression and clinical outcome via Kaplan-Meier survival analysis.

Molecular dynamics of chemotactic signalling orchestrates dental pulp stem cell fibrosis during aging.

Aging often triggers dental pulp fibrosis, resulting in clinical repercussions such as increased susceptibility to dental infections, compromised tooth vitality, and reduced responsiveness to dental interventions. Despite its prevalence, the precise molecular mechanisms underlying this condition remains unclear. Leveraging single-cell transcriptome analysis from both our own and publicly available datasets, we identified Ccrl2 macrophages as particularly vulnerable during the early stages of aging.

2 km Uncompressed HD Video Wireless Transmission at 100 GHz Based on All-Optical Frequency Up- and Down-Conversion.

The millimeter-wave wireless transmission system is widely regarded as a promising solution for applications of future 6G communication. This paper presents an experimental comparison between all-optical and all-electric receivers for millimeter-wave communication systems over a 15 m wireless link and demonstrates 200 m and 2 km real-time uncompressed HD video transmission using an all-optical transceiver at 100 GHz. The systems leverage photonics-assisted heterodyne beating techniques at the transmitter, while the receivers employ either an avalanche photodiode (APD)-based all-optical approach or an envelope detection-based all-electric approach.

Long noncoding RNA Gm4419 promotes mesangial cell proliferation and extracellular matrix accumulation the miR-455-3p/histone deacetylase 2 axis.

Long noncoding RNAs may function as competitive endogenous RNAs by sponging microRNAs, thereby contributing to the progression of diabetic nephropathy. In this study, a potential diabetic nephropathy-related long noncoding-microRNA-mRNA axis, Gm4419-miR-455-3p-, was predicted using bioinformatics methods. To verify the role of the Gm4419-miR-455-3p- axis in diabetic nephropathy, an high glucose-induced mesangial cell model was established.

Ultrasound-guided Genicular Nerve Block in Patients Undergoing Knee Arthroscopy: A Randomized Controlled Trial.

This study was to investigate the efficacy of ultrasound-guided genicular nerve block for patients who underwent knee arthroscopy. Patients were randomized into two groups: 1. nerve block group: ultrasound-guided genicular nerve block (superomedial, superolateral and inferomedial genicular nerve, 2-ml 0.

Platelet Membrane-Coated HGF-PLGA Nanoparticles Promote Therapeutic Angiogenesis and Tissue Perfusion Recovery in Ischemic Hindlimbs.

Therapeutic angiogenesis has garnered significant attention as a potential treatment strategy for lower limb ischemic diseases. Although hepatocyte growth factor (HGF) has been identified as a key promoter of therapeutic angiogenesis, its clinical application is limited due to its short half-life. In this study, we successfully developed and characterized platelet membrane-coated HGF-poly(lactic--glycolic acid) (PLGA) nanoparticles (NPs).

π-HuB: the proteomic navigator of the human body.

The human body contains trillions of cells, classified into specific cell types, with diverse morphologies and functions. In addition, cells of the same type can assume different states within an individual's body during their lifetime. Understanding the complexities of the proteome in the context of a human organism and its many potential states is a necessary requirement to understanding human biology, but these complexities can neither be predicted from the genome, nor have they been systematically measurable with available technologies.

Can the Short-Form UCLA Loneliness Scale Be Used to Measure Loneliness Among Chinese Older Adults? From Classical Test Theory to Rasch Analysis.

Background: Loneliness among older adults, is a subjective experience and a public health issue in aging societies. Psychometrically sound and culturally sensitive measures are needed for developing precisely targeted interventions in culturally distinct groups. This study tested the Short-Form UCLA Loneliness Scale (ULS-8) among Chinese older adults.

The globular domain of extracellular histones mediates cytotoxicity via membrane disruption mechanism.

Histones are traditionally recognized for structuring nuclear architecture and regulating gene expression. Recent advances have revealed their roles in inflammation, coagulation, and immune responses, where they act as damage-associated molecular patterns. The mechanisms by which histones induce membrane leakage are not well understood, and certain cells, including endothelial cells and peritoneal macrophages, show resistance to histone-mediated pore formation.

Synthetic augmentation of cancer cell line multi-omic datasets using unsupervised deep learning.

Integrating diverse types of biological data is essential for a holistic understanding of cancer biology, yet it remains challenging due to data heterogeneity, complexity, and sparsity. Addressing this, our study introduces an unsupervised deep learning model, MOSA (Multi-Omic Synthetic Augmentation), specifically designed to integrate and augment the Cancer Dependency Map (DepMap). Harnessing orthogonal multi-omic information, this model successfully generates molecular and phenotypic profiles, resulting in an increase of 32.

The diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis.

Objective: To investigate the diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis (IM).

Methods: A total of 135 children with IM as the IM group and 100 healthy volunteers as the healthy group were included in this retrospective study. Peripheral blood lymphocyte subsets marked as CD3+, CD4+, CD8+, CD16 + CD56+, and CD19 + in the peripheral blood were quantified using flow cytometry.

Textbook oncological outcome of locally advanced gastric cancer patients with preoperative sarcopenia: a multicenter clinical study.

Background: The impact of postoperative sarcopenia on the Textbook Oncological Outcome (TOO) in locally advanced gastric cancer (LAGC) remains uncertain. This study investigates the relationship between sarcopenia and TOO, explores its long-term prognostic value, and develops a prognostic model incorporating sarcopenia and TOO for survival prediction.

Methods: We performed a retrospective analysis of clinical and pathological data from patients with LAGC who underwent radical surgery at two Chinese tertiary referral hospitals.