Investigating the possible mechanism of Cornus officinalis in the therapy of ischemic stroke by UHPLC-Q-TOF-MS, network pharmacology, molecular docking, and experimental verification.

Ethnopharmacological Relevance: Cornus officinalis is a conventional Chinese medicine for tonifying liver and kidney in ancient China. The active ingredients from Cornus officinalis can delay the progression of cerebral aneurysms, alleviate experimental autoimmune encephalomyelitis, and show a good intervention effect on brain diseases. Loganin, the active ingredient of Cornus officinalis, has a neuroprotective effect on cerebral ischemia-reperfusion injury in mice.

sp. nov., a novel actinobacterium isolated from the gill of the leopard coral grouper ().

A novel actinomycete, designated strain ZYX-F-249, was isolated from the gill of a leopard coral grouper in Yongxing Island, Hainan Province, China. Based on 16S rRNA gene sequence analysis, strain ZYX-F-249 belonged to the genus , with high similarities to ATCC 12950 (98.7 %), 211020 (98.

New Isocoumarins from an Endophytic Fungal Strain Diaporthe arengae M2 and their Antibacterial Activities.

Four new isocoumarin derivatives 12-O-acetyl-isocitreoisocoumarinol (1), (+)-(10R)-O-acetyl-diaportinol (2-a), (-)-(10S)-O-acetyl-diaportinol (2-b), peyroisocoumarin E (3) and new stereoconfigurations of three isocoumarin derivatives desmethyldichlorodiaportinol A (4), threo-monochlorodiaportinol A (5-a), erytheo-monochlorodiaportinol A (5-b), together with nine known ones (6-14), were separated from the rice fermentation of endophytic fungus Diaporthe arengae M2 isolated from Camellia oleifera. The structures of new compounds were determined by extensive spectroscopic analyses including nuclear magnetic resonance (NMR) and high resolution electrospray ionization mass spectroscopy (HR-ESI-MS). Compounds 4, 7, 8, 12, 13 exhibited definite inhibition against five strains of bacteria with the MIC values range from 16 μg/mL to 64 μg/mL.

Persteroid, a new steroid from the marine-derived fungus sp. ZYX-Z-143.

A new steroid named persteroid () and seven known compounds (-) were isolated from the marine-derived fungus sp. ZYX-Z-143. The structure of was determined by HRESIMS, NMR, and ECD calculations.

Antibacterial Indole-Diterpenoid Alkaloids from the Marine Fungus Penicillium sp. ZYX-Z-718.

Two new indole-diterpenoids, penpaxilloids F and G (1 and 2), along with 11 known analogues (3-13), were isolated from the marine fungus Penicillium sp. ZYX-Z-718. The structures of the new compounds were identified by extensive spectroscopic analyses including HR-ESI-MS, UV, and NMR, as well as theoretical NMR chemical shifts and ECD calculations.

sp. nov., isolated from marine sediment.

A novel actinobacterium, strain ZYX-F-186, was isolated from marine sediment sampled on Yongxing Island, Hainan Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain ZYX-F-186 belongs to the genus , with high similarity to KK1-3 (98.3 %), K11-0047 (98.

Diaporchalasins A-E, New Cytochalasins from the Endophytic Fungus Diaporthe sp. BMX12 Isolated from Aquilaria sinensis.

Five new cytochalasins, diaporchalasins A-E (1-5), together with 14 known congeners (6-19) were isolated from the endophytic fungus Diaporthe sp. BMX12, which was isolated from the branches of Aquilaria sinensis. The structures of the new compounds were elucidated by extensive spectroscopic analyses including high-resolution electron spray ionization mass spectrometry (HR-ESI-MS) and nuclear magnetic resonance (NMR).

Anti-diabetic and anti-inflammatory indole diterpenes from the marine-derived fungus Penicillium sp. ZYX-Z-143.

Seven new indole-diterpenoids, penpaxilloids A-E (1-5), 7-methoxypaxilline-13-ene (6), and 10-hydroxy-paspaline (7), along with 20 known ones (8-27), were isolated from the marine-derived fungus Penicillium sp. ZYX-Z-143. Among them, compound 1 was a spiro indole-diterpenoid bearing a 2,3,3a,5-tetrahydro-1H-benzo[d]pyrrolo[2,1-b][1,3]oxazin-1-one motif.

Diverse indole-diterpenoids with protein tyrosine phosphatase 1B inhibitory activities from the marine coral-derived fungus Aspergillus sp. ZF-104.

Eight previously undescribed indole-diterpenoids named penerpenes O-V (1-8), together with seven known analogues (9-14), were isolated from the marine soft coral-derived fungus Aspergillus sp. ZF-104. Their structures including the absolute configurations of these compounds were assigned on the basis of spectroscopic data and ECD analysis along with quantum ECD and NMR calculations.

Lanostane triterpenoids with anti-proliferative and PTP1B/α-glucosidase inhibitory activities from the fruiting bodies of Ganoderma calidophilum.

Twelve previously undescribed and four known lanostane triterpenoids were isolated from the fruiting bodies of Ganoderma calidophilum. The structures of undescribed compounds, ganodecalones H-S (1-12), were elucidated by extensive spectroscopic analysis as well as ECD and NMR calculations. Compound 4 showed significant inhibitory activity against human leukaemia cell line K562, gastric cancer cell line SGC-7901, and cervical cancer cell line HeLa with IC values of 13.

Chemical constituents of the marine-derived fungus AN-13.

Two new compounds named 3()-hydroxy-1-(2,4,5-trihydroxy-3,6- dimethylphenyl)-hex-4-en-1-one () and acremonilactone (), together with nine known compounds (-), were isolated from the fermentation broth of . associated with marine sediments collected from South China Sea. NMR and HRESIMS spectroscopic analysis elucidated the structure of two new compounds.

sp. nov., isolated from marine sediment.

A novel actinobacterium strain (M4I6) was isolated from marine sediment collected in Megas Gialos, Syros, Greece. On the basis of 16S rRNA gene sequence analysis, strain M4I6 was indicated as belonging to the genus , with high similarity to '' LAM7112 (97.9 %), IFO 15555 (97.

Two new alkaloids from the endophytic fungus sp. HM230 isolated from Maxim.

Endophytic fungi is an important source for the discovery of bioactive natural compounds. A chemical investigation of the ethyl acetate extract of the endophytic fungus sp. HM230 derived from stems of the herb Maxim led to isolation of five alkaloids, including two new compounds, schizophyllins M () and N (), along with three known ones (-).

Antidiabetic lanostane triterpenoids from the fruiting bodies of Ganoderma weberianum.

Eight previously undescribed lanostane triterpenoids, ganodeweberiols A ∼ H (1-8), together with eighteen known compounds (9-26), were isolated from the fruiting bodies of Ganoderma weberianum. The structures and absolute configurations of the new compounds were determined by extensive spectroscopic analysis, as well as NMR chemical shifts and electronic circular dichroism (ECD) calculations. Compounds 2, 7, 12, and 14 showed significant α-glucosidase inhibitory activity with IC values ranging from 35.

Phenethoxy Derivatives with Anti-inflammatory Activities from the Betelnut Endophytic G10.

Eight new phenethoxy derivatives, trichoasperellins A-H (-), were isolated from the endophytic fungus G10 isolated from the medicinal plant L. The structures of these compounds were elucidated from spectroscopic data, -based configurational analysis, and Mosher's methods. Compounds - and - bear one or two multioxidized C moieties with the same carbon skeleton.

Two lanostane triterpenoids with -glucosidase inhibitory activity from the fruiting bodies of .

A new oxygenated lanostane-type triterpenoid, 20,24-epoxy-lanosta-7,9(11)-dien-3,15,25,26-tetraol (), together with three known compounds (-) were isolated from the fruiting bodies of . Extensive NMR spectrometry and HRESIMS analysis, as well as NMR and ECD calculations elucidated the structure of the new compound. 27-nor-3-hydroxylanosta-7,9(11),23-trien-25-one () showed superior -glucosidase inhibitory activity with IC value of 122.

Humulane-Type Macrocyclic Sesquiterpenoids From the Endophytic Fungus sp. of .

Three new humulane-type sesquiterpenoids, penirolide A (), penirolide B (), and 10-acetyl-phomanoxide (), together with three known compounds aurasperone A (), pughiinin A (), and cyclo(l-Leu-l-Phe) () were isolated from the endophytic fungus sp derived from the leaves of L. Their structures including their absolute configurations were determined based on the analysis of NMR and HRESIMS spectra, NMR chemical shifts, and ECD calculations. Compounds , , , and significantly inhibited glucagon-induced hepatic glucose production, with EC values of 33.

Cytotoxic Indole-Diterpenoids from the Marine-Derived Fungus sp. KFD28.

Four new indole-diterpenoids, named penerpenes K-N (-), along with twelve known ones (-), were isolated from the fermentation broth produced by adding L-tryptophan to the culture medium of the marine-derived fungus sp. KFD28. The structures of the new compounds were elucidated extensively by 1D and 2D NMR, HRESIMS data spectroscopic analyses and ECD calculations.

Triterpenoids and meroterpenoids with α-glucosidase inhibitory activities from the fruiting bodies of Ganoderma australe.

Macrofungi Ganoderma is a valuable medicinal fungus resource for human health and longevity in China. In this study, ten undescribed compounds including seven lostane-type triterpenoids, ganodaustralic acids A ∼ G (1-7), one pair of meroterpenoid enantiomers, (-)-6'-O-ethyllingzhiol (8) and (+)-6'-O-ethyllingzhiol (9), and one polyhydroxylated sterol, 3-O-acetyl-fomentarol C (10), together with eight known compounds (11-18), were isolated from the fruiting bodies of Ganoderma australe. The structures of the new compounds were elucidated by extensive spectroscopic analysis as well as NMR and electronic circular dichroism (ECD) calculations.

Three New Quinazoline-Containing Indole Alkaloids From the Marine-Derived Fungus sp. HNMF114.

By feeding tryptophan to the marine-derived fungus sp. HNMF114 from the bivalve mollusk , 3 new quinazoline-containing indole alkaloids, named aspertoryadins H-J (-), along with 16 known ones (-), were obtained. The structures of the new compounds were elucidated by the analysis of spectroscopic data combined with quantum chemical calculations of nuclear magnetic resonance (NMR) chemical shifts and electron capture detector (ECD) spectra.

[Study on chemical constituents from fruiting bodies of Ganoderma calidophilum].

Chemical constituents were isolated and purified from fruiting bodies of Ganoderma calidophilum by various column chromatographic techniques, and their chemical structures were identified through combined analysis of physicochemical properties and spectral data. As a result, 11 compounds were isolated and identified as(24E)-lanosta-8,24-dien-3,11-dione-26-al(1), ganoderone A(2), 3-oxo-15α-acetoxy-lanosta-7,9(11), 24-trien-26-oleic acid(3),(23E)-27-nor-lanosta-8,23-diene-3,7,25-trione(4), ganodecanone B(5), ganoderic aldehyde A(6), 11β-hydroxy-lucidadiol(7), 3,4-dihydroxyacetophenone(8), methyl gentiate(9), ganoleucin C(10), ganotheaecolumol H(11). Among them, compound 1 is a new triterpenoid.

Two new indole-diterpenoids from the marine-derived fungus sp. KFD28.

Two new compounds named epipaxilline () and penerpene J () were isolated from the marine-derived fungus sp. KFD28. Their structures including absolute configurations were determined on the basis of spectroscopic methods and ECD analysis.

Three new C steroidal glycosides isolated from and their potential inhibitory effects on tyrosine protein kinases.

Three new steroidal glycosides, metapregnoside A-C (-), together with one known compound, byzantionoside B (), were isolated from the fresh whole herb of by using high-speed countercurrent chromatography and semi-preparative liquid chromatography. Their structures and relative configurations were elucidated by spectroscopic methods including 1D NMR, 2D NMR and HR-ESI-MS. The potential targets of compound - were identified by virtual screening.