Characterization of a betaendornavirus isolated from the edible fungus Morchella sextelata.

A positive-sense single-stranded RNA mycovirus was isolated from Morchella sextelata strain ZY-1 and designated as "Morchella sextelata endornavirus 1" (MsEV1). The complete genome of MsEV1 was found to be 16,589 nucleotides (nt) in length and to contain a single large open reading frame (ORF) encoding the viral proteins PRK00409, transmethylase, DEXDc, helicase, and RdRP in a polyprotein of 5,477 amino acids. Phylogenetic analysis based on RdRP nucleotide sequences revealed that this endornavirus is a member of the genus Betaendornavirus in the family Endornaviridae.

Dissipative Particle Dynamics: Simulation of Chitosan-Citral Microcapsules.

In this paper, the dissipative particle dynamics (DPD) method is used to simulate the self-assembly process, appearance, mesoscopic structure, and wrapping properties of microcapsules formed with citral as the core material and chitosan and sodium alginate as the single-wall materials, and with citral as the core material and chitosan-sodium alginate, chitosan-methylcellulose, sodium alginate-chitosan, and sodium alginate-methylcellulose as the double-wall materials. The effects of chitosan content and wall material composition on the structure, morphology, encapsulation performance, and stability of microcapsules are compared and analyzed. In addition, the microcapsules are deeply analyzed by using the mesoscopic structure, radial distribution function, and diffusion coefficient.

Tumor Prognostic Risk Model Related to Monocytes/Macrophages in Hepatocellular Carcinoma Based on Machine Learning and Multi-Omics.

Tumor-associated macrophages (TAMs) are crucial in hepatocellular carcinoma (HCC) development and invasion. This study explores monocyte/ macrophage-associated gene expression profiles in HCC, constructs a prognostic model based on these genes, and examines its relationship with drug resistance and immune therapy responses. Single-cell RNA sequencing(scRNA-seq) data from 10 HCC tissue biopsy samples, totaling 24,597 cells, were obtained from the GEO database to identify monocyte/macrophage-associated genes.

Lighting up metal nanoclusters by the HO-dictated electron relaxation dynamics.

The modulation of traps has found attractive attention to optimize the performance of luminescent materials, while the understanding of trap-involved photoluminescence management of metal nanoclusters greatly lags behind, thus extensively impeding their increasing acceptance as the promising chromophores. Here, we report an efficient passivation of the structural oxygen vacancies in AuAg nanoclusters by leveraging the HO molecules, achieving a sensitive color tuning from 536 to 480 nm and remarkably boosting photoluminescence quantum yield from 5.3% (trap-state emission) to 91.

Electromagnetic black holes with controllable composite right/left-handed transmission lines.

Given that the Hawking radiation from celestial black holes is extremely weak and thus difficult to be practically detected, a series of analogue black holes have been constructed to observe the relevant analogue Hawking radiations in the laboratory. Based on the critical behavior of group velocity of the microwave signal propagating along a controllable composite right/left-handed transmission line, in this paper, we theoretically demonstrate that an electromagnetic black hole (EBH) can be constructed in the relevant co-moving coordinate system, i.e.

Treating human cancer by targeting EZH2.

Enhancer of zeste homolog 2 (EZH2), an epigenetic regulator that primarily inhibits downstream gene expression by tri-methylating histone H3, which is usually overexpressed in tumors and participates in many processes such as tumor occurrence and development, invasion, migration, drug resistance, and anti-tumor immunity as an oncogene, making it an important biomarker in cancer therapy. Collectively, several transcription factors and RNAs cooperate to facilitate the elevated expression of EZH2 in cancer. Although the significance of blocking EZH2 in cancer for inhibiting cancer progression is widely recognized, the clinical application of EZH2 inhibitors continues to encounter numerous challenges.

Acute abdominal pain as the initial presenting symptom of pediatric Burkitt lymphoma: A case report and literature review.

Rationale: Acute abdominal pain is a prevalent clinical symptom in pediatric patients, primarily attributed to pediatric acute abdomen. However, there are rare exceptional instances where neoplastic diseases may cause acute abdominal pain.

Patient Concerns: The present study encompasses a case of pediatric Burkitt lymphoma wherein acute abdominal pain manifested as the initial symptom, erroneously diagnosed as acute appendicitis.

Computational and Experimental Elucidation of the Charge-Dependent Acid-Etching Dynamics and Electrocatalytic Performance of Au(SR) (q = -1, 0, +1) Nanoclusters.

Using thiolate-protected Au(SR) nanocluster (NC) with different charge states as the test candidate, how the charge effect affects the etching dynamics of thiolate ligands in acid and the electrocatalytic performance is explored. The ab initio molecular dynamics (AIMD) simulations revealed the charge-dependent reaction kinetics in acid, where the anionic and neutral Au(SCH) (q = -1, 0) favorably react with the acid and partially remove the thiolate ligands via two-step proton attack, while the cationic Au(SCH) NC is acid-resistant with no tendency for -SR removal. Density functional theory (DFT) calculations further predict that the dethiolated Au sites exhibit enhanced catalytic activity for CO electroreduction to CO, with the anionic Au showing significantly superior activity.

KAT8 facilitates the proliferation of cancer cells through enhancing E7 function in HPV-associated cervical cancer.

Persistent human papillomavirus (HPV) infection serves as the principal etiological factor in cervical cancer, with the oncoprotein E7, which is encoded by the virus, playing a key role in tumorigenesis. However, targeted therapeutic strategies against E7 remain underexplored. KAT8, a lysine acetyltransferase, significantly contributes to oncogenesis through the regulation of transcription.

Enhanced control of surface wave transitions via trapped air film on multiscale structured surfaces.

The control of the threshold for surface wave transition is a topic of great interest in both scientific and industrial communities. Traditional methods, like installing baffles, for suppressing surface waves often suffer from issues including increased system weight, lack of flexibility and universality, and problems with structural performance. This study utilizes micro/nanoscale surface modifications and millimeter scale slot structure design to trap air film to absorb vibration energy under liquid surface waves.

Discovery of a novel Wnt inhibitor DK419: Reversing temozolomide resistance in glioblastoma by switching off Wnt/β-catenin signaling pathway to inhibit MGMT expression.

Temozolomide (TMZ) remains the primary oral chemotherapeutic agent for glioblastoma, but its efficacy is hampered by resistance mechanisms involving O-methylguanine-DNA methyltransferase (MGMT). MGMT repairs the TMZ-induced lethal O-methylguanine (O-MeG) lesions, leading to treatment resistance. Current small molecule covalent MGMT inhibitors have limited clinical application due to severe hematological toxicity when used with TMZ.

Dihydro-β-agarofuran sesquiterpenoids from the root bark of Tripterygium wilfordii and their anti-neuroinflammatory activities.

A phytochemical study of Tripterygium wilfordii root bark was conducted 25 novel dihydro-β-agarofuran sesquiterpenoids (1-25) and 20 known analogues (26-45). Structural analysis elucidated by comprehensive spectroscopic analysis, including X-ray crystallography and electronic circular dichroism (ECD). Anti-neuroinflammatory assessments in BV-2 cells revealed certain compounds effectively suppressed tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6).

Immunogenicity and protection of recombinant self-assembling ferritin-hemagglutinin nanoparticle influenza vaccine in mice.

Purpose: Influenza virus remains a serious burden to global public health. Current influenza vaccine fails to provide impeccable protection efficacy to the annual seasonal influenza and cannot offer a timely response to potential pandemic influenza. It is necessary to develop next generation influenza vaccines to solve the current dilemma.

Gastrointestinal complications of hepatic glycogen storage disease: a national survey questionnaire study in China.

Background: Hepatic glycogen storage diseases (GSD) are inborn errors of metabolism with abnormal storage or utilization of glycogen, a complex disease with significant genetic heterogeneity and similar clinical manifestations. This study aimed to describe the gastrointestinal symptoms and endoscopic features of hepatic GSD, including types Ia, Ib, III, VI, and IX, to provide evidence for etiology and treatment.

Methods: A national cohort survey questionnaire was distributed to patients diagnosed with GSD type Ia, Ib, III, VI, and IX through genetic testing or their parents in mainland China in May 2022.

Ligand-induced changes in the electrocatalytic activity of atomically precise Au nanoclusters.

Atomically precise gold nanoclusters have shown great promise as model electrocatalysts in pivotal electrocatalytic processes such as the hydrogen evolution reaction (HER) and carbon dioxide reduction reaction (CORR). Although the influence of ligands on the electronic properties of these nanoclusters is well acknowledged, the ligand effects on their electrocatalytic performances have been rarely explored. Herein, using [Au(SR)] nanoclusters as a prototype model, we demonstrated the importance of ligand hydrophilicity hydrophobicity in modulating the interface dynamics and electrocatalytic performance.

Understanding the Ag-S interface stability and electrocatalytic activity of CO electroreduction in atomically precise Ag nanoclusters.

Elucidating the catalytic properties of metal nanoclusters (NCs) with essentially the same structure but different core metals is of fundamental interest. Our current studies have demonstrated that the thiolated Ag(SR) NC exhibits SR ligand leaching dynamics and electrocatalytic activity in CO reduction distinct from those of its Au(SR) NC structural analogue.

The impact of diabetes on Sepsis-induced cardiomyopathy.

Purpose: This study investigated the association between diabetes and Sepsis-induced cardiomyopathy (SIC), focusing on how changes in inflammatory response and cardiac function influence SIC prognosis. The aim is to provide clinicians with more accurate treatment and management strategies, ultimately enhancing patient outcomes and quality of life.

Methods: This retrospective cohort study analyzed 258 Sepsis-induced cardiomyopathy (SIC) patients, stratified by diabetes status and HbA1C levels.

Risk factors for brain metastasis in lung cancer: an umbrella review of systematic reviews and meta-analyses.

Objectives: To conduct an umbrella review to extensively evaluate and summarise the evidence regarding the relationship between risk factors and the occurrence of brain metastasis in lung cancer.

Design: Umbrella review of systematic reviews and meta-analyses.

Data Sources: Four databases (PubMed, EMBASE, Web of Science and Cochrane Library) were searched from inception to 10 November 2024.

Understanding the Role of Potential and Cation Effect on Electrocatalytic CO Reduction in All-Alkynyl-Protected Ag Nanoclusters.

Atomically precise metal nanoclusters (NCs) have emerged as an intriguing class of model catalysts for electrochemical CO reduction reactions (CORR). However, the interplay between the interface environment (e.g.

Rapid and Visual Detection of Urinary Pathogens by Employing Bifunctional Deoxyribonucleic Acid Sensors and Aggregation of Gold Nanoparticles.

A simple, rapid, and visual approach is developed to perform diagnosis of urinary tract infection (UTI) and antimicrobial susceptibility testing (AST) by employing smart bifunctional DNA (bfDNA) sensors, exonuclease III, concatermers of CuO nanoparticles (CuONPs), and gold NPs (AuNPs) aggregation [AuNPs agglutination (AA)], namely, the bfDEC-AA method. The bfDNA sensors serve as probes for identifying 16S rRNA genes of bacterium or 18S rRNA of fungus and as mediators connecting the concatermers of CuONPs. The AA as a signal source is triggered by Cu(I)-catalyzed azide-alkyne cycloaddition click chemistry.

Norcrohalane A, an Unusual C nor-Halimane-Type Diterpenoid from Croton crassifolius.

An unusual C nor-halimane-type diterpenoid, norcrohalane A (1), a new nor-lasserane-type sesquiterpenoid, norcrolasane A (2), along with four known compounds (3-6) were isolated from the roots of Croton crassifolius. Compound 1 represents the first example of a C nor-halimane-type diterpenoid, which features a rare 6/6/6 tricyclic ring system, and the A ring is aromatic. Compound 2 is a rare nor-lasserane-type sesquiterpenoid.

High-adhesion ovarian cancer cell resistance to ferroptosis: The activation of NRF2/FSP1 pathway by junctional adhesion molecule JAM3.

Ovarian cancer remains a significant challenge due to the lack of effective treatment and the resistance to conventional therapies. Ferroptosis, a form of regulated cell death characterized by iron-depend and lipid peroxidation, has emerged as a potential therapeutic target in cancer. Ovarian cancer has been reported to exert an "iron addiction" phenotype which makes it is susceptible to ferroptosis inducers.

What drives Chinese youth to use fitness-related health information on social media? An analysis of intrinsic needs, social media algorithms, and source credibility.

Introduction: The role of social media in providing fitness-related health information has been widely discussed; however, there is a notable lack of research on fitness-related health information behaviors among youth within the social media context. This study aims to address this gap by integrating Self-Determination Theory (SDT)-based internal factors and external factors (social media algorithms and source credibility).

Methods: A voluntary sample of 600 participants, aged 15 to 29, was recruited.

Quantifying the global biodiversity of Proterozoic eukaryotes.

The global diversity of Proterozoic eukaryote fossils is poorly quantified despite its fundamental importance to the understanding of macroevolutionary patterns and dynamics on the early Earth. Here we report a new construction of fossil eukaryote diversity from the Paleoproterozoic to early Cambrian based on a comprehensive data compilation and quantitative analyses. The resulting taxonomic richness curve verifies Cryogenian glaciations as a major divide that separates the "Boring Billion" and Ediacaran periods, with the former characterized by a prolonged stasis, and the latter by greater diversity, more-rapid turnover, and multiple radiations and extinctions.

Ferrocene-Functionalized Atomically Precise Metal Clusters Exhibit Synergistically Enhanced Performance for CO Electroreduction.

The integration of organometallic compounds with metal nanoparticles can, in principle, generate hybrid nanocatalysts endowed with augmented functionality, presenting substantial promise for catalytic applications. Herein, we synthesize an atomically precise metal cluster (AgCu) catalyst integrated with alkynylferrocene molecules (AgCu-Fc). This hybrid catalyst design facilitates a continuous electron transfer channel via an ethynyl bridge and establishes a distinctive local chemical environment, resulting in remarkably enhanced catalytic activity in CO electroreduction.