Exploring the gut microbiota and metabolome of Lateolabrax japonicus: A multi-omics approach.

The intestinal microbiota plays a crucial role in the health and development of fish, engaging in intricate interactions with the host organism. As a significant species in aquaculture, Lateolabrax japonicus serves as an exemplary model for investigating these interactions and their subsequent effects on growth and health. This study utilized a multi-omics approach, incorporating metagenomic sequencing and non-targeted metabolomics, to delineate the gut microbiota and metabolome of L.

Lignin and copper nanocomposite coating for antibacterial mask.

Lignin is a natural phenolic polymer characterized with renewable, sustainable and biocompatible, but yet remain underutilized. In the post-pandemic era, people are conventionally reusing mask but without any disinfections to prevent infection of virus in public places, which could lead to accumulation of bacteria and secondary infections. The development of antibacterial mask from lignin would simultaneously address the hygiene issues of used mask due to microbe accumulation and provide novel approach for lignin valorization.

Acinetobacter corruptisaponis sp. nov., Isolated from a Spoiled Bath Lotion.

Strain DM2021935 representing a novel Acinetobacter species was isolated from a spoiled bath lotion in Guangdong, China. Based on 16S rRNA gene phylogenetic analysis, strain DM2021935 was closely related to 'Acinetobacter thutiue' VNH17, Acinetobacter junii CIP 64.5, and Acinetobacter tibetensis Y-23.

Lignin hydrogenolysis: Tuning the reaction by lignin chemistry.

Replacing fossil resource with biomass is one of the promising approaches to reduce our carbon footprint. Lignin is one of the three major components of lignocellulosic biomass, accounting for 10-35 wt% of dried weight of the biomass. Hydrogenolytic depolymerization of lignin is attracting increasing attention because of its capacity of utilizing lignin in its uncondensed form and compatibility with the biomass fractionation processes.

Rapid detection of in based on CRISPR-Cas13a coupled with recombinase polymerase amplification.

The principal pathogen responsible for chronic urinary tract infections, immunocompromised hosts, and cystic fibrosis patients is , which is difficult to eradicate. Due to the extensive use of antibiotics, multidrug-resistant has evolved, complicating clinical therapy. Therefore, a rapid and efficient approach for detecting strains and their resistance genes is necessary for early clinical diagnosis and appropriate treatment.

Selecting Suitable Near-Native Lignins for Research.

There are several methods to isolate near-native lignins, including milled-wood lignin, enzymatic lignin, cellulolytic enzyme lignin, and enzymatic mild-acidolysis lignin. Which one is the most representative of the native lignin? Herein, near-native lignins were isolated from different plant groups and structurally analyzed to determine how well these lignins represented their native lignin counterparts. Analytical methods were applied to understand the molecular weight, monomer composition, and distribution of interunit linkages in the structure of the lignins.

Fundicoccus culcitae sp. nov., a novel potential bacteriocin producing bacterium isolated from a spoiled eye mask.

A Gram-positive, facultatively anaerobic, oval beaded-shape, oxidase-negative, and non-motile bacterium designated DM20194951 was isolated from a spoiled eye mask obtained from Guangdong, China. Based on the 16S rRNA gene sequence, phylogenetic analysis indicated that strain DM20194951 showed the highest sequence similarity (95.8%) to Fundicoccus ignavus WS4937.

Geraniol attenuates virulence factors by inhibiting quorum sensing of .

is a ubiquitous opportunistic pathogen that can cause severe respiratory tract infections. Geraniol, a chemical component of essential oils, has antimicrobial and anti-inflammatory activities, along with low toxicity. However, the effect and mechanism of geraniol against virulence factors are rarely studied.

heteroresistance to levofloxacin caused by upregulated expression of essential genes for DNA replication and repair.

(), a common cause of severe chronic infections, has developed heteroresistance to several antibiotics, thus hindering successful treatment. In this study, we aimed to investigate the characteristics and mechanisms underlying levofloxacin (LVX) heteroresistance in PAS71 and PAS81 clinical isolates using a combination of physiological and biochemical methods, bacterial genomics, transcriptomics, and qRT-PCR. The six strains, namely PAS71, PAS72, PAS81, PAS82, ATCC27853, and PAO1, were studied.

High value valorization of lignin as environmental benign antimicrobial.

Lignin is a natural aromatic polymer of -hydroxyphenylpropanoids with various biological activities. Noticeably, plants have made use of lignin as biocides to defend themselves from pathogen microbial invasions. Thus, the use of isolated lignin as environmentally benign antimicrobial is believed to be a promising high value approach for lignin valorization.

The interplays between epigallocatechin-3-gallate (EGCG) and Aspergillus niger RAF106 based on metabolism.

Epigallocatechin-3-gallate (EGCG) is a vital kind of catechin with high bioactive activities, however, limited research has been conducted to elucidate the molecular basis of EGCG biotransformation by Aspergillus niger and the underlying regulatory mechanisms. In this study, A. niger RAF106, isolated from Pu-erh tea, was applied to conduct the EGCG fermentation process, and the samples were collected at different fermentation times to determine the intermediary metabolites of EGCG and the metabolome as well as physiological activity changes of A.

Outer membrane protein of OmpF contributes to swimming motility, biofilm formation, osmotic response as well as the transcription of maltose metabolic genes in Citrobacter werkmanii.

Bacterial outer membrane proteins (Omps) are essential for environmental sensing, stress responses, and substance transport. Our previous study discovered that OmpA contributes to planktonic growth, biocide resistance, biofilm formation, and swimming motility in Citrobacter werkmanii, whereas the molecular functions of OmpF in this strain are largely unknown. Thus, in this study, the ompF gene was firstly knocked out from the genome of C.

ompX contribute to biofilm formation, osmotic response and swimming motility in Citrobacter werkmanii.

Citrobacter werkmanii, an aerobe and mesophilic Proteobacterium, is universal in industrial putrefaction, coastal water, and human blood. Our previous studies have discovered that outer membrane protein X (OmpX) of C. werkmanii is involved in calcium response, but the underlying mechanisms and its molecular characteristics remain elusive.

Proteomic signatures of synergistic interactions in antimicrobials.

Mounting evidence has shown that antimicrobial agents can interfere synergistically with bacterial viability and proliferation when acting together at both the planktonic and biofilm levels without clear underlying molecular mechanisms. Here, multiplexed proteomics by iTRAQ was used to study the interplay between two biocides, the isothiazolone 1,2-benzisothiazolin-3-one (BIT) and the chelating agent disodium ethylenediaminetetraacetic acid (EDTA-2Na), employing the Citrobacter werkmanii as a model system. We first confirmed that these two biocides act synergistically on this bacterial species and then extracted the proteomic profiles of C.

The role of structural evolution in the complexation and flocculation of heavy metals by the microbial product poly-γ-glutamic acid.

The process and mechanism of heavy metal flocculation with extracellular polymeric substances (EPS) secreted by microorganisms, are crucial to their fate in natural environment, wastewater treatment and soil bioremediation applications. However, the structural features of EPS and the relationship between these features and the flocculation process and mechanism remain unclear. In the present study, structural features of the microbial product poly-γ-glutamic acid (γ-PGA) complexed with the heavy metal ions Pb and Cu were characterized and the evolution of these features was identified as having a key role in the flocculation process and mechanism.

Chemical Components of Aqueous Extracts of Fruits and Their Effects on The Transcriptome of .

is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of fruits inhibit the planktonic growth and initial biofilm formation of in a dose-dependent manner. Moreover, the biofilm topologies became sparse and decreased as the concentration of the aqueous extracts increased.

Synthesis, molecular docking, and evaluation of antibacterial activity of 1,2,4-triazole-norfloxacin hybrids.

A series of 1,2,4-triazole-norfloxacin hybrids was designed, synthesized, and evaluated for in vitro antibacterial activity against common pathogens. All the newly synthesized compounds were characterized by Fourier-transform infrared spectrophotometry, proton and carbon nuclear magnetic resonance, and electrospray ionization-mass spectrometry. Representative compounds from each step of the synthesis were further characterized by X-ray crystallography.

Roles of ompA of Citrobacter werkmanii in bacterial growth, biocide resistance, biofilm formation and swimming motility.

The genus Citrobacter is commonly found in environmental and industrial settings, some members of which have been used for bioremediation of heavy metals owing to the absorption ability of their biofilms. Although our previous studies have found that the outer membrane protein A (OmpA) contributes to the process of Citrobacter werkmanii biofilm formation, the underlying mechanisms remain elusive. Therefore, we deleted ompA from the genome of C.

Detoxification of ionic liquids using glutathione, cysteine, and NADH: Toxicity evaluation by Tetrahymena pyriformis.

Ionic liquids (ILs), also known as green solvents, are widely acknowledged in several fields, such as chemical separation, synthesis, and electrochemistry, owing to their excellent physiochemical properties. However, their poor biodegradability may lead to environmental and health risks, posing a severe threat to humans, thus requiring further research. In this study, the biotoxicities of the imidazolium-based ILs were evaluated in Tetrahymena pyriformis.

Crystal structure of bis-{2-hy-droxy-'-[1-(pyrazin-2-yl)ethyl-idene]benzohydrazidato}cadmium(II).

In the title complex mol-ecule, [Cd(CHNO)], the Cd atom is coordinated in a distorted octa-hedral geometry by two tridentate ligands synthesized from 2-hy-droxy-benzohydrazide and 1-(pyrazin-2-yl)ethan-1-one. The mol-ecule has twofold crystallographic symmetry and is isomorphous to its Mn, Co, Ni, Cu and Zn counterparts.

Diallyl sulfide from garlic suppresses quorum-sensing systems of Pseudomonas aeruginosa and enhances biosynthesis of three B vitamins through its thioether group.

Diallyl sulfide (DAS) and diallyl disulfide (DADS), two constituents of garlic, can inhibit quorum sensing (QS) systems of Pseudomonas aeruginosa. However, the differences in the mechanism of QS inhibition between DAS and DADS, and the functional chemical groups of these sulfides that contribute in QS inhibition have not been elucidated yet. We assumed that the sulfide group might play a key role in QS inhibition.

Crystal structures and biological properties of aroylhydrazone Ni(II) complexes.

Three aroylhydrazone ligands ((Z)-N'-([2,2'-bithiophen]-5-ylmethylene)-2-hydroxybenzohydrazide, HL1; (Z)-N'-([2,2'-bithiophen]-5-ylmethylene)-3-hydroxybenzohydrazide, HL2; and (Z)-N'-([2,2'-bithiophen]-5-ylmethylene)-4-hydroxybenzohydrazide, HL3) and their complexes with nickel (Ni(L1), 1; Ni(L2), 2; Ni(L3)∙DMF, 3) were synthesized and characterized by ESI-MS, NMR, IR, UV-vis and elemental analysis techniques. The molecular structure of ligand (HL2) and complexes 1-3 was confirmed by single crystal X-ray crystallography. The single crystal X-ray structure of complexes 1-3 showed a distorted square planar geometry around the metal center, and the ligands adopt a bidentate chelating mode.

Nanocopper-loaded Black phosphorus nanocomposites for efficient synergistic antibacterial application.

Novel nanocopper-loaded black phosphorus (BP/Cu) nanocomposites were synthesized to synergistically exert enhanced antibacterial activities aimed at reducing antibiotics abuse. First, both BP and Cu display low biotoxicity, broadening their application in the microbiological field. Second, the unique electronic properties of BP enable BP/Cu nanocomposites to amplify antibacterial effects via interfacial charge transfer, resulting in a surge of reactive oxygen species (ROS).