Publications by authors named "Qing Sha"
Single-cell transcriptomic analysis of immune cell dynamics in the healthy human endometrium.
Biochem Biophys Rep
September 2024
Article Synopsis
- The endometrial immune system is key for successful placental implantation and a healthy pregnancy, but the roles of immune cells throughout the reproductive cycle are not yet fully understood.
- A comprehensive analysis of over 230,000 single-cell transcriptomes from endometrial samples reveals that natural killer (NK) cells have significant growth and differentiation potential, with variations in activity during different stages of the reproductive cycle.
- The study identifies important immune interactions and specific risk genes tied to reproductive diseases, offering potential biomarkers and insights for understanding changes during pregnancy.
Article Synopsis
- Mitotic chromosomes maintain some active chromatin features during cell division, a process known as "mitotic bookmarking," to help reestablish transcriptional programs after mitosis.
- Using single-cell sequencing techniques, researchers studied over 6000 human liver cells and observed that chromatin accessibility fluctuated during cell division, decreasing until metaphase and then increasing post-segregation.
- Certain chromatin regions remained consistently open through mitosis, and genes in these areas were found to be quickly reactivated after cell division, with the nuclear transcription factor NF-YA playing a key role in this process.
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease, and CD4 T cells are known to promote SLE development. Here, we explore heterogeneities in the CD4 T cell regulome and their associations with SLE pathogenesis by performing assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) and single-cell transcriptome sequencing (single-cell RNA sequencing [scRNA-seq]) of peripheral CD4 T cells from 72 SLE patients and 30 healthy controls. Chromatin accessibility signatures of CD4 T cells are correlated with disease severity.
View Article and Find Full Text PDFBackground: Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease that involves a variety of cell types. However, how the epigenetic dysregulations of peripheral immune cells contribute to the pathogenesis of RA still remains largely unclear.
Results: Here, we analysed the genome-wide active DNA regulatory elements of four major immune cells, namely monocytes, B cells, CD4 T cells and CD8 T cells, in peripheral blood of RA patients, osteoarthritis (OA) patients and healthy donors using Assay of Transposase Accessible Chromatin with sequencing (ATAC-seq).
Maintaining homeostasis of the decidual immune microenvironment at the maternal-fetal interface is essential for placentation and reproductive success. Although distinct decidual immune cell subpopulations have been identified under normal conditions, systematic understanding of the spectrum and heterogeneity of leukocytes under recurrent miscarriage in human deciduas remains unclear. To address this, we profiled the respective transcriptomes of 18,646 primary human decidual immune cells isolated from patients with recurrent pregnancy loss (RPL) and healthy controls at single-cell resolution.
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