In-Cell Fast Photochemical Oxidation Interrogates the Native Structure of Integral Membrane Proteins.

Integral membrane proteins (IMPs) are pivotal for cellular functions but challenging to investigate. Here, IC-FPOMP (in-cell fast photochemical oxidation of MPs) is introduced, a method enabling in situ footprinting of IMPs within live cells. IC-FPOMP generates reactive oxygen radicals from various precursors (TiO nanoparticles or HO) near the membrane.

Development of coumarin and procaine linked hybrid molecules as a novel class of SHP1 fluorescent activators.

The development of small fluorescent organic molecules used in bioimaging experiment has boomed the progress of molecular and cellular biology, providing new and efficient tools to elucidate a myriad of cellular and multicellular processes. In this work, a class of fluorescent activators against SHP1 was designed and synthesized for the first time. The representative compound 3n showed activating effect against SHP1 with EC of 17.

Mobile phone addiction and negative emotions: an empirical study among adolescents in Jiangxi Province.

Background: The relationship between mobile phone addiction and negative affect among adolescents is characterized by intricate interconnections. These factors have an impact on the emotional and psychological well-being of young people. While previous studies have provided preliminary insights into this relationship, further in-depth exploration and research is required to fully understand the complex mechanisms behind these relationships and to identify any group differences.

Bodyweight and Absolute Lymphocyte Count-Based Dosing of Rabbit Anti-thymocyte Globulin Results in Early CD4 Immune Reconstitution in Patients with Inborn Errors of Metabolism Undergoing Umbilical Cord Blood Transplantation.

Background: Rabbit anti-thymocyte globulin (rATG) decreases the risk of graft failure and graft-versus-host disease (GVHD) in a setting of allogenic hematopoietic cell transplantation (HCT) but has highly variable pharmacokinetics. Recently, it was shown that a dosing nomogram based on recipient bodyweight and absolute lymphocyte count reduced rATG overexposure, which led to faster immune reconstitution. The aim of this study is to evaluate the feasibility and benefits of using an rATG dosing nomogram to achieve early CD4 immune reconstitution in pediatric patients with inborn errors of metabolism (IEM) undergoing umbilical cord blood transplantation.

Differences in Combustible Cigarette Smoking-Related Biomarkers by Hormonal Contraceptive Use: An Exploratory Study.

Introduction: Hormonal contraceptives (HCs), which contain synthetic forms of estrogen (i.e., ethinyl estradiol) and/or progesterone (i.

Molecular basis of vitamin-K-driven γ-carboxylation at the membrane interface.

The γ-carboxylation of glutamate residues enables Ca-mediated membrane assembly of protein complexes that support broad physiological functions, including haemostasis, calcium homeostasis, immune response and endocrine regulation. Modulating γ-carboxylation levels provides prevalent treatments for haemorrhagic and thromboembolic diseases. This unique post-translational modification requires vitamin K hydroquinone (KH) to drive highly demanding reactions catalysed by the membrane-integrated γ-carboxylase (VKGC).

The Impact of on Oral and Systemic Health: A Comprehensive Review of Recent Research.

Oral diseases, particularly dental caries and periodontal disease, pose significant global health challenges. The imbalance of the oral microbiota plays a key role in the occurrence of these diseases, prompting researchers to seek new strategies to restore oral ecological balance. is a Gram-positive rod-shaped bacterium that exists in various body parts of humans, including the gastrointestinal tract, urinary tract, skin, and so on.

Development and Validation of KCPREDICT: A Deep Learning Model for Early Detection of Coronary Artery Lesions in Kawasaki Disease Patients.

Kawasaki disease (KD) is a febrile vasculitis disorder, with coronary artery lesions (CALs) being the most severe complication. Early detection of CALs is challenging due to limitations in echocardiographic equipment (UCG). This study aimed to develop and validate an artificial intelligence algorithm to distinguish CALs in KD patients and support diagnostic decision-making at admission.

Glycoprotein 350-targeted chimeric antigen receptor T-cell therapy for nonneoplastic chronic active Epstein-Barr virus infection: a case report.

Background: Chronic active Epstein-Barr virus (CAEBV) infection is a rare disease in which the Epstein-Barr virus (EBV) persists and replicates, causing chronic symptoms and fatal complications. The treatment of CAEBV is still evolving. Our case report showed a new therapy for CAEBV.

CMPK2 promotes NLRP3 inflammasome activation via mtDNA-STING pathway in house dust mite-induced allergic rhinitis.

Background: House dust mite (HDM) is the leading allergen for allergic rhinitis (AR). Although allergic sensitisation by inhaled allergens renders susceptible individuals prone to developing AR, the molecular mechanisms driving this process remain incompletely elucidated.

Objective: This study aimed to elucidate the molecular mechanisms underlying HDM-induced AR.

Parental Acceptance of Human Papillomavirus Vaccination Among Females Treated for Childhood Cancer and/or With Hematopoietic Stem Cell Transplantation: A Cross-Sectional Study.

Background: Survivors of childhood cancer (CCS) and hematopoietic stem cell transplantation (HSCT) recipients are at increased risk of human papillomavirus (HPV)-associated malignancies. Although HPV vaccination is recommended for these groups, parental acceptance remains uncertain.

Procedure: We recruited caregivers of female CCS/HSCT aged ≥9 years from the Shanghai Children's Medical Center (SCMC) vaccination clinic.

The glutathione S-transferase BnGSTU12 enhances the resistance of Brassica napus to Sclerotinia sclerotiorum through reactive oxygen species homeostasis and jasmonic acid signaling.

Sclerotinia sclerotiorum is a severe disease that affects rapeseed (Brassica napus), resulting in significant yield losses. In previous study, we identified the candidate GLUTATHIONE S-TRANSFERASE (GST) gene, BnGSTU12, associated with sclerotiorum stem resistance and the expression levels of BnGSTU12 in resistant lines were higher than that in susceptible lines. We analyzed the function of the BnGSTU12 during S.

Red blood cell distribution width at admission and the short-term mortality of patients with severe burn injury: a meta-analysis.

Objective: Red blood cell distribution width (RDW) is a marker of inflammation and oxidative stress, and its elevation has been associated with poor outcomes in critically ill patients. This meta-analysis aimed to evaluate the association between RDW at admission and short-term mortality in patients with severe burn injury.

Methods: We systematically searched PubMed, Embase, and Web of Science up to August 2024, following PRISMA 2020 and Cochrane guidelines.

Solubility-Equilibrium-Assisted Kinetic Resolution Polymerization toward Isotactic Polyesters Containing Axial Chirality.

High-level control over polymer stereochemistry leverages the fine-tuning of material properties, but it is still a formidable challenge in synthetic polymer chemistry. Herein we prepared a new class of salph yttrium catalysts bearing axially chiral binaphthyl moieties for axially stereocontrolled polymerization of -Me-DBO. ()-bearing bulkier binaphthyl units accomplished moderate isoselectivity via kinetic resolution polymerization, affording P(Me-BDO) with a of up to 0.

Clinical outcomes of Omicron infection and vaccine acceptance among pediatric liver transplant recipients: insights from a cross-sectional survey.

Objectives: Our study aims to explore the clinical characteristics of Omicron infection in pediatric liver transplant recipients (PLTRs), after the national COVID-19 outbreak. Additionally, we will investigate changes in vaccine coverage and parental attitudes towards vaccinating their children after this current outbreak.

Methods: We conducted a web-based questionnaire survey to gather information on Omicron infection, vaccination status, and guardian attitude among PLTRs.

Expert consensus for pertussis in children: new concepts in diagnosis and treatment.

Background: Pertussis resurgence has been reported worldwide in the past two decades. Pertussis is still endemic and difficult to control though with universal vaccination in children. The resurgence may be related to multiple variables, such as increased disease awareness and laboratory tests, waning of immunity following vaccination, and/or genetic mutations of Bordetella pertussis.

Controllable Graphene/MoS Heterointerfaces by Perpendicular Surface Functionalization.

Surface chemistry and interface interactions profoundly influence the properties of two-dimensional (2D) materials and heterostructures. Therefore, developing methods to precisely control surfaces and interfaces is crucial for harnessing the properties and functions of 2D materials and heterostructures. Here, we developed a facile approach to tuning the interface distance and properties of graphene/MoS heterostructures (G/MoS) by varying the functional groups attached to the surface of graphene bottom layer.

Low-cost portable sensor for rapid and sensitive detection of Pb ions using capacitance sensing integrated with microfluidic enrichment.

Lead ion (Pb) pollution is a critical global issue due to its ability to accumulate in the human body, resulting in severe health problems. Despite extensive research efforts devoted to the detection of Pb contamination, practical, rapid, and field-deployable sensors for Pb is yet to be developed to effectively safeguard the environment and public health. Herein, a label-free affinity-based sensing device is developed based on printed circuit board (PCB) for low-cost, easy-to-use, and real-time on-site detection of Pb ions.

Fabrication and characterization of lysozyme fibrils/Zein complexes for resveratrol encapsulation: Improving stability, antioxidant and antibacterial activities.

Resveratrol (Res), a naturally occurring hydrophobic polyphenol, boasts numerous health-promoting bio-functionalities. However, its limited water solubility and stability impede further applications in the food industry. This study aims to address these challenges by fabricating stable Res-loaded lysozyme fibrils/zein (Ly-F/Z) complexes.

Using dredged sediments from Lake Taihu as a plant-growing substrate: Focusing on the impact of microcystins.

Using dredged sediment as plant growth substrates is a promising way to deal with large amounts of excavated sediments. However, it is a big challenge to deal with various pollutants in sediments, among which microcystins (MCs) gained limited attention. In this study, sediments collected from Lake Taihu were mixed with agricultural soil at a 1:1 ratio to create various growing substrates for lettuce (Lactuca sativa L.

Solution-processable ordered defect compound semiconductors for high-performance electronics.

Solution-processable semiconductors hold promise in enabling applications requiring cost-effective electronics at scale but suffer from low performance limited by defects. We show that ordered defect compound semiconductor CuInSe, which forms regular defect complexes with defect-pair compensation, can simultaneously achieve high performance and solution processability. CuInSe transistors exhibit defect-tolerant, band-like transport supplying an output current above 35 microamperes per micrometer, with a large on/off ratio greater than 10, a small subthreshold swing of 189 ± 21 millivolts per decade, and a high field-effect mobility of 58 ± 10 square centimeters per volt per second, with excellent uniformity and stability, superior to devices built on its less defective parent compound CuInSe, analogous binary compound InSe, and other solution-deposited semiconductors.

Comparative genomic analysis of pathogenic factors of Listeria spp. using whole-genome sequencing.

Listeria monocytogenes is an important foodborne pathogen known for causing listeriosis. To gain insights into the pathogenicity, genetic characterization, and evolution of various Listeria species, in vitro cell adhesion and invasion ability assays and whole-genome sequencing were performed using four Listeria strains isolated from livestock and poultry slaughterhouses. The four Listeria strains exhibited adhesion and invasion abilities in Caco-2 and RAW264.