MiR-21-5p promotes differentiation and reduces oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11.

Objective: This study aimed to investigate the function of miRNA 21-5p in regulating the differentiation of C2C12 myoblasts and intramuscular lipid droplet accumulation in myotubes.

Methods: The role of miR-21-5p in the proliferation and differentiation of myofibroblasts and intracellular lipid accumulation was analyzed using bioinformatics, CCK-8 assay, RT-qPCR, immunoblotting, immunofluorescence staining, and Oil Red O staining.

Results: Analysis of porcine BodyMap transcriptomic data revealed differential expression of miRNA 21-5p in skeletal muscle and adipose tissue.