Diurnal expression of CXC receptors 4 (CXCR4) and CXC chemokine ligand 12 (CXCL12) in .

The CXC chemokine ligand 12/CXC receptor 4 ligand/receptor interaction is the most ancient chemokine system in vertebrates, and it plays a pivotal role in the immune system's response against bacterial infection. In the current study, 1211 bp CXCR4 and 937 bp CXCL12 genes, which encode 364 and 99 amino acids, respectively, were isolated. Within the 24-hour light/dark cycle, the maximum of CXCR4 in the intestine, spleen, and anterior kidney of occurs at 8:00, 16:00, and 16:00, respectively.

Diurnal rhythm expression of transcription factor NF-κB subunit p65 in Pelteobagrus vachellii.

The pleiotropic transcription factor nuclear factor-kappa B (NF-κB) has important functions in viral resistance. In the present study, we isolated a p65 subunit of NF-κB cDNA from Pelteobagrus vachellii (termed Pvp65) and characterized it. The full-length p65 cDNA comprised 3651 bp, including a 148-bp 5'-untranslated region (UTR), a 106-bp 3'-UTR, and an open reading frame encoding a 1067-amino acid putative protein.

Effects of dietary hydroxyproline on collagen metabolism, proline 4-hydroxylase activity, and expression of related gene in swim bladder of juvenile Nibea diacanthus.

This study was conducted to investigate the effects of dietary hydroxyproline (Hyp) on tissue collagen level, proline 4-hydroxylase (P4H) activity as well as transcript levels of COL1As (COL1A1 and COL1A2) and P4Hαs (P4Hα(I), P4Hα(II), and P4Hα(III)) in juvenile Nibea diacanthus. A total of 450 fishes were randomized to six equal groups and fed the diet with graded supplementary Hyp-0, 5, 10, 15, 20, and 25 g kg of dry matter for 8 weeks. Results showed that fish fed diets with 10 g kg Hyp had significantly higher acid-soluble collagen (ASC) and total collagen (TC) concentrations in swim bladder than fish fed with the other diets (P < 0.