Simultaneous and High-Throughput Analytical Strategy of 30 Fluorinated Emerging Pollutants Using UHPLC-MS/MS in the Shrimp Aquaculture System.

This study established novel and high-throughput strategies for the simultaneous analysis of 30 fluorinated emerging pollutants in different matrices from the shrimp aquaculture system in eastern China using UHPLC-MS/MS. The parameters of SPE for analysis of water samples and of QuEChERS methods for sediment and shrimp samples were optimized to allow the simultaneous detection and quantitation of 17 per- and polyfluoroalkyl substances (PFASs) and 13 fluoroquinolones (FQs). Under the optimal conditions, the limits of detection of 30 pollutants for water, sediment, and shrimp samples were 0.

A novel approach based on supramolecular solvents microextraction for quick detection of perfluoroalkyl acids and their precursors in aquatic food.

Per-and polyfluoroalkyl substances (PFASs) have garnered significant attention owing to their prevalence and adverse effects on humans. The direct dietary intake of perfluoroalkyl acids (PFAAs) and PFAAs precursors (pre-PFAAs) biotransformation are considered major contributors to human exposure to PFASs. However, little information is available on analytical methods for the simultaneous detection of PFAAs and pre-PFAAs.

Accurate and stable detection of p-phenylenediamine antioxidants and their transformation products in aquatic products using antioxidant protection - Analysis of actual aquatic products.

Given the high toxicity of N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) derivatives, such as 6PPD quinone (6PPDQ) to salmon, as well as their ubiquitous presence in the environment, the contaminant of aquatic food products has drawn significant attention. However, analytical methods for p-Phenylenediamines (PPDs) and their transformation products (TPs) in aquatic products remain underdeveloped. In particular, the degradation of some compounds and strong matrix effects complicate detection.

Assessing Seasonal Effects on Identification of Cultivation Methods of Short-Growth Cycle L. Using IRMS and NIRS.

Seasonal (temporal) variations can influence the C, H, O, and N values and nutrient composition of organic (ORG), green (GRE), and conventional (CON) vegetables with a short growth cycle. Stable isotope ratio mass spectrometry (IRMS) and near-infrared spectroscopy (NIRS) combined with the partial least squares-discriminant analysis (PLS-DA) method were used to investigate seasonal effects on the identification of ORG, GRE, and CON L. samples (s).

Hepatotoxicity evaluation and possible mechanisms of decabrominated diphenyl ethers (BDE-209) in broilers: Oxidative stress, inflammatory, and transcriptomics.

Decabrominated diphenyl ether (BDE-209), a persistent organic pollutant, is linked to a great number of health problems, the most severe of which impact the liver due to its role in the elimination and degradation of exogenous harmful substances. Though the hepatotoxicity of BDE-209 has been observed, its underlying mechanism is yet unknown. The purpose of this study is to thoroughly investigate the hepatotoxicity of BDE-209 and its molecular processes in broilers by subjecting 120 male broilers to varied concentrations of BDE-209 for 42 days.

Determination of 25 polybrominated diphenyl ethers in Chinese mitten crab ecosystems by gas chromatography-mass spectrometry.

A sensitive and reliable method for determining 25 polybrominated diphenyl ethers (PBDEs) in Chinese mitten crabs and their ecosystems ranging from the growing environment to edible feed by gas chromatography coupled to triple quadrupole mass spectrometry with advanced electron ionization (GC-AEI-MS/MS) was developed and validated. Accelerated solvent extraction (ASE) and liquid-liquid extraction were used to extract solid and water samples, respectively. On the basis of a traditional acid-base silica column, deactivated silica was added and -hexane elution was used to increase the effect of separation and purification.

Effects of Thiamethoxam and Fenvalerate Residue Levels on Light-Stable Isotopes of Leafy Vegetables.

Accurate identification of the rational and standardized use of pesticides is important for the sustainable development of agriculture while maintaining a high quality. The insecticides thiamethoxam and fenvalerate and the vegetables spinach, cabbage, and lettuce were used here as study objects. Descriptive analysis and primary reaction kinetic equations were used to analyze the changes in metabolic residues of the two insecticides after different numbers of application in three vegetables.

Dissipation, Bioconcentration and Dietary Risk Assessment of Thiamethoxam and Its Metabolites in and Substrates under Different Application Methods.

In order to acquire scientific evidence for the application of thiamethoxam (TMX) in cultivation, residue and dissipation experiments for field trials were performed with the application of TMX in compost and casing soil, respectively. An effective QuEChERS method was established to analyze TMX and its two metabolites, clothianidin (CLO) and thiamethoxam-urea (TMX-urea), in compost, casing soil, and fruiting bodies. The results indicated that the TMX dissipation half-lives (t) at dosages of 10 and 50 mg kg were 19.

Toxicokinetics and edible tissues-specific bioaccumulation of decabrominated diphenyl ethers (BDE-209) after exposure to the broilers.

Decabromodiphenyl ether (BDE-209), the primary constituent of a widely used flame retardant formulation, is often present in high levels in avian derived products and could be transferred to humans through consumption. The purpose of this study was to investigate the toxicokinetics and bioaccumulation patterns of BDE-209 in different tissues of broilers, which would benefit the evaluation of chicken product safety. Male broilers received a single oral administration of BDE-209 at 25 mg/kg.

Toxic effects and transcriptional responses in zebrafish liver cells following perfluorooctanoic acid exposure.

As a typical type of persistent organic pollutant, perfluorooctanoic acid (PFOA) is pervasive in the environment. Multiple studies have found that PFOA has hepatotoxicity, but the mechanism remains poorly understood. In this study, the toxic effects of different concentrations of PFOA on zebrafish liver cells were systematically assessed by recording cell survival, ultrastructural observations, and transcriptome analyses.

Efficient Removal of Nonylphenol Contamination from Water Using Optimized Magnesium Silicate.

Nonylphenol (NP) is considered to be an environmentally toxic, endocrine-disrupting chemical that affects humans and ecosystems. Adsorption is one of the most promising approaches for the removal of nonylphenol contamination from water. Herein, in order to design an adsorbent with high adsorption capacity, magnesium silicate with different Mg/Si ratios was successfully synthesized by a sol-gel method at 60 °C.

New insights into the transfer and accumulation of dioxins and dioxin-like PCBs in the food web of farmed Chinese mitten crabs: A typical case from the Yangtze River area.

Dioxins and dioxin-like polychlorinated biphenyls (DL-PCBs) transfer and accumulation behavior remains poorly understood in the farmed Chinese mitten crab (Eriocheir sinensis). In this study, dioxins and DL-PCBs concentration in 48 farming crabs in lower reaches of the Yangtze River was monitored and controlled field design was conducted in a typical farm to dissect the dioxins and DL-PCBs contamination in crab food web (crab, feeds, and environment). Results showed that dioxins and DL-PCBs were ubiquitous in farmed crabs with concentrations ranging from 0.

The immunotoxicity of decabromodiphenyl ether (BDE-209) on broiler chicks by transcriptome profiling analysis.

Decabromodiphenyl ether (BDE-209) has drawn significant attention due to its suppression of immune functions in animals and even humans. In order to explore the mechanism through which BDE-209 affects the immune system, broiler chicks were fed a diet containing various concentrations of BDE-209 (0, 0.004, 0.

Cloning and characterisation of a Δ9 fatty acyl desaturase-like gene from the red claw crayfish (Cherax quadricarinatus) and its expression analysis under cold stress.

Desaturase is one of the key enzymes in the unsaturated fatty acid synthesis pathway. Δ9 desaturase catalyzes the synthesis of oleic acid from stearic acid by introducing double bonds in the 9th and 10th carbon chains, thereby increasing the content of MUFAs in the body. In order to explore the main function of the Δ9 desaturase gene under low temperature stress, RACE-PCR technology was used in this study to clone the full-length sequence of the CqFAD9-like from the hepatopancreas of red claw crayfish, Cherax quadricarinatus.

Study on the Dynamic Difference between Single and Mixed Residues of Three Neonicotinoids in L.

Multiple insecticides' residues after the mixed application of several neonicotinoids cause combined pollution and bring new challenges to food safety and pest control during agricultural production. In this study, three neonicotinoid insecticides, namely imidacloprid (IMI), acetamiprid (ACE), and thiamethoxam (TMX), were mixed and evenly sprayed on L. in the field.

BDE-47 induces nephrotoxicity through ROS-dependent pathways of mitochondrial dynamics in PK15 cells.

2,2',4,4'-tetrabromodiphenyl ether (BDE-47)-induced nephrotoxicity is closely associated with oxidative stresses and mitochondrial abnormalities. Mitochondrial fusion and fission dynamics are crucial for maintaining mitochondrial and cellular physiological homeostasis. However, the detailed mechanisms through which BDE-47 disrupts this dynamic and contributes to renal injuries are still not fully understood.

Toxic effects of Decabromodiphenyl ether (BDE-209) on thyroid of broiler chicks by transcriptome profile analysis.

The wide usage of decabromodiphenyl ether (BDE-209) results in its increasing occurrence in the environment and increasing attention in regard to human and animal health. BDE-209 is an endocrine disruptor for hypothyroidism, but the toxicity mechanism is unclear. Here, the histopathology and transcriptome sequencing of thyroid tissue from broiler chicks were investigated by supplemental feeding with different concentrations of BDE-209 for 42 days (0-4 g/kg in basal diet), followed by determining the levels of thyroid hormones in serum.

Determination of Polybrominated Diphenyl Ethers in Water Samples Using Effervescent-Assisted Dispersive Liquid-Liquid Icroextraction with Solidification of the Aqueous Phase.

An effective and sensitive method is necessary for the determination of polybrominated diphenyl ethers (PBDEs) pollutants in water. In this study, effervescent-assisted dispersive liquid-liquid microextraction with solidification of the aqueous phase (EA-DLLME-SAP), followed by Gas Chromatography-Tandem Mass Spectrometry (GC-MS-MS) quantitative analysis, was established for the preconcentration and determination of PBDEs in real environmental water samples. 1,1,2,2-Tetrachloroethane was used as the extractant and directly dispersed into the water phase of the aqueous samples with the aid of a large number of carbon dioxide bubbles generated via the acid-base reaction of acetic acid and sodium bicarbonate, which did not require the use of a dispersant during the extraction process.

Effects of Decabrominated Diphenyl Ether Exposure on Growth, Meat Characteristics and Blood Profiles in Broilers.

Decabrominated diphenyl ether (BDE-209) is widely used as a flame retardant and is detected at high levels in the environment. Its toxicities have been reported and have attracted attention. In the present study, broilers were used to determine the response in growth performance, carcass traits, meat quality, blood profiles and antioxidant system to BDE-209 exposure at doses of 0, 0.

Nephrotoxicity and possible mechanisms of decabrominated diphenyl ethers (BDE-209) exposure to kidney in broilers.

The flame retardant decabrominated diphenyl ether (BDE-209) is a widely used chemical in a variety of products and exists extensively in the environment. BDE-209 has been reported to induce kidney injury and dysfunction. However, the causes and mechanisms of its nephrotoxicity are still under investigation.

Exposure and health risk assessment of secondary contaminants closely related to brominated flame retardants (BFRs): Polybrominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) in human milk in shanghai.

Polybrominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs), as the secondary environmental pollutants of the widely used brominated flame retardants (BFRs), possess the similar physicochemical and toxic properties as polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs). However, studies on human body exposure to them are extremely limited. In this study, forty human milk samples collected in Shanghai were measured for 13 PBDD/F congeners using gas chromatography-high resolution mass spectrometry (GC-HRMS), to investigate their exposure level and characteristics, potential source and corresponding health risks to breastfed infants.

Isotope chemometrics determines farming methods and geographical origin of vegetables from Yangtze River Delta Region, China.

Shanghai city has encountered possible food fraud regarding the geographical mislabeling of vegetables for economic gain. A combination of δC, δN, δH and δO values and partial least squares discrimination analysis and support vector machine (SVM) methods were used for the first time to assess farming methods and determine the origin of vegetables from Shanghai city, Anhui and Zhejiang provinces. The results showed that 65.

Assessing the combined toxicity effects of three neonicotinoid pesticide mixtures on human neuroblastoma SK-N-SH and lepidopteran Sf-9 cells.

Neonicotinoid insecticides have been widely used in plant protection against pests worldwide. Generally, more than one neonicotinoids are detected in plants and foods, and such mixtures may show combined toxicity and increase the risk for both insects and higher organisms. In this study, the combined toxicity of imidacloprid (IM), acetamiprid (AC) and thiamethoxam (TH) was investigated using human neuroblastoma cell line (SK-N-SH) and lepidopteran cell line (Sf-9).

Imidacloprid dissipation, metabolism and accumulation in Agaricus bisporus fruits, casing soil and compost and dietary risk assessment.

An improved understanding of imidacloprid (IMI) metabolism and accumulation in casing soil or compost-mushroom systems will help to optimise the safe use of IMI for agricultural pest control in Agaricus bisporus cultivation. In this study, the dissipation, metabolites and accumulation of IMI in casing soil or compost-A. bisporus systems were investigated.