Implications of baseline glycemic control by plasma glycated hemoglobin A1c on adverse outcomes in patients with coronary heart disease and type 2 diabetes mellitus: Results from the PROMISE study.

Background: The optimal glycosylated hemoglobin (HbA1c) target in type 2 diabetes mellitus (T2DM) patients remains controversial, especially in patients with concomitant coronary heart disease (CHD). This study aimed to investigate the correlation between baseline HbA1c and long-term prognosis in CHD patients with T2DM.

Methods: The study enrolled 6,839 CHD patients with T2DM and measured HbA1c at admission in a multicenter prospective observational cohort.

Precise Metabolomics Defines Systemic Metabolic Dysregulation Distinct to Acute Myocardial Infarction Associated With Diabetes.

Background: Acute myocardial infarction (AMI) is a leading cause of death and disability. Diabetes is an important risk factor and a common comorbidity in AMI patients. The higher mortality risk of diabetes-AMI relative to nondiabetes-AMI indicates a need for specific treatment to improve clinical outcome.

[Study on the growth of Vibrio cholerae O139 within Acanthamoeba polyphaga and its survival in the cysts in low temperature].

Objective: To determine whether Acanthamoeba polyphaga could affect the survival and growth of Vibrio cholerae O139 in low temperature.

Methods: V. cholerae O139 was co-cultured with the Acanthamoeba polyphaga to be examined on its intracellular growth and survival rate within cysts at low temperature, using methods as Gram-staining, electron microscope and passage culture.

[A micro-culture method for continuous observation of free-living amoebae].

Objective: To establish a method for co-culture of amoebae and endosymbionts, also for continuously observing the microphenotype of amoebae.

Methods: 24 wells culture plate with cover glass on the wells was used as containers. Amoebae and Candida albicans were co-cultured in microdrop of medium in the wells at 37 degrees C, and observed under x1000.

[Survival and growth of Vibrio cholerae O139 inside Acanthamoeba].

Objective: To study the survival and growth of Vibrio cholerae inside the Acanthamoeba polyphage.

Methods: Survival and growth of Vibro cholerae O139, co-cultured with Acanthamoeba polyphaga, was observed inside the trophozoites and cysts, using Gram stain and electron microscope.

Results: Viable O139 was observed inside the amoebal vacuoles in 24 hours.

[The discovery of naked cluster particles of Parachlamydia and its developmental mechanism].

Objective: To study the survival and developmental morphology of Parachlamydia (BN9) within Acanthamoeba.

Methods: The morphology of BN9 within Acanthamoeba was studied by inverted phase contrast microscope, electron microscope, Gimenez and AO-staining with amoebal co-culture.

Results: The endosomal maturation-blocked were formed after the egress of BN9.