Publications by authors named "Qin-Mei Ruan"
Identification and validation of reference genes for quantitative real-time PCR studies in Hedera helix L.
Plant Physiol Biochem
November 2016
Article Synopsis
- Reference gene evaluation is essential for accurate gene expression analysis in RT-qPCR, particularly in lesser-studied plants like the medicinal Hedera helix L.
- The study identified and evaluated 14 candidate reference genes for stability across different plant tissues and abiotic stress conditions using geNorm and NormFinder, marking the first such evaluation in H. helix.
- The findings highlighted the need for selecting specific reference genes based on experimental conditions, revealing F-box and 40S as the most stable genes overall and under ABA treatment, respectively, while also confirming the reliability of the selected reference genes for future RT-qPCR studies.