Publications by authors named "Qin Tjokrosurjo"
CD8+ T cells differentiate into two subpopulations in response to acute viral infection: memory precursor effector cells (MPECs) and short-lived effector cells (SLECs). MPECs and SLECs are epigenetically distinct; however, the epigenetic regulators required for formation of these subpopulations are mostly unknown. In this study, we performed an in vivo CRISPR screen in murine naive CD8+ T cells to identify the epigenetic regulators required for MPEC and SLEC formation, using the acute lymphocytic choriomeningitis virus Armstrong infection model.
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- In vivo T cell screens are essential for understanding immunity, but there's currently no agreed-upon design for their implementation, including factors like gene library size, the amount of cells transferred, and the number of mice used in experiments.
- The Framework for In vivo T cell Screens (FITS) is introduced to standardize these parameters, ensuring robust and effective experimental outcomes across various contexts.
- As a practical application, the researchers used FITS to enhance a CD8+ T cell screen in a tumor model, incorporating unique molecular identifiers (UMIs) to boost statistical analysis and monitor T cell behavior linked to different gene knockouts.
Annotation of immunologic gene function in vivo typically requires the generation of knockout mice, which is time consuming and low throughput. We previously developed CHimeric IMmune Editing (CHIME), a CRISPR-Cas9 bone marrow delivery system for constitutive, ubiquitous deletion of single genes. Here we describe X-CHIME, four new CHIME-based systems for modular and rapid interrogation of gene function combinatorially (C-CHIME), inducibly (I-CHIME), lineage-specifically (L-CHIME) or sequentially (S-CHIME).
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